With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L p...With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.展开更多
In the traditional dual-frequency radar ranging system,there is a contradiction between ranging precision and ranging distance.So a tri-frequency ranging method is proposed.The principle of the tri-frequency ranging i...In the traditional dual-frequency radar ranging system,there is a contradiction between ranging precision and ranging distance.So a tri-frequency ranging method is proposed.The principle of the tri-frequency ranging is that the distance can be achieved by transmitting the three frequencies and then measuring the three phase difference and calculating the number of wavelength.What's more,range ambiguity will not occur within a certain distance.The experimental results show that,if the waveband is 0.47 ~0.57 m,the measurement of unambiguous distance of 25 ~500m can be realized.If the wavelength tolerance error is less than ± 10^(-7) and the phase error is less than ± 10^(-3),the precision of the distance ranging can reach 10^(-6).展开更多
文摘With the progress of plant genome research, more than 50 plant metallothionein_like (MT_L) genes have been found, but only several MT_L proteins have been detected and no experimental structural information for MT_L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal_sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine_rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT_L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT_L protein LSC54 was then modeled with this method.
基金Supported by the National Natural Science Foundation of China(No.61107052)the National Natural Science Foundation of Tianjing(No.11JCYBJC00100)
文摘In the traditional dual-frequency radar ranging system,there is a contradiction between ranging precision and ranging distance.So a tri-frequency ranging method is proposed.The principle of the tri-frequency ranging is that the distance can be achieved by transmitting the three frequencies and then measuring the three phase difference and calculating the number of wavelength.What's more,range ambiguity will not occur within a certain distance.The experimental results show that,if the waveband is 0.47 ~0.57 m,the measurement of unambiguous distance of 25 ~500m can be realized.If the wavelength tolerance error is less than ± 10^(-7) and the phase error is less than ± 10^(-3),the precision of the distance ranging can reach 10^(-6).
