Objective To investigate protective effects of electroacupuncture on injury of lipid peroxidation-induced by liver ischemia in rats with sepsis.Methods Forty-eight male SD rats were subjected to sepsis by cecal ligati...Objective To investigate protective effects of electroacupuncture on injury of lipid peroxidation-induced by liver ischemia in rats with sepsis.Methods Forty-eight male SD rats were subjected to sepsis by cecal ligation and puncture (CLP),and were randomly divided into a sham operation group (group A),a CLP model group (group B),a CLP model plus electroacupuncture (EA) at Zúsānlǐ (足三里 ST 36) group (group C),a CLP model plus electroacupuncture at a sham acupoint group (group D),a CLP model with vagotomy group (group E) and a CLP model plus electroacupuncture group after vagotomy (group F),with 8 rats in each group.CLP was performed after abdominal vagotomy in group E and group F.EA (2 mA,2/100 Hz) was applied at bilateral "Zúsānlǐ" (足三里 ST 36) in group C and group F,and at sham acupoints in group D,respectively,for 1 hour.The hepatic blood flow (HBF) was detected by a laser-doppler flowmetry at 6 hours after CLP.The activity of plasma alanine transarninase (ALT) was determined;contents of hepatic malondialdehyde (MDA),activity of xanthine oxidase (XOD) and the rate of water content in the hepati tissue were assessed,respectively.Results Hepatic blood flow was (56.97±11.95) U in group C,which was significantly lower than (80.12± 19.57) U shown in group A,but higher than (42.61±10.97) U in group B,(44.53±9.23) U in group D,(30.05 ±4.46) U in group E and (30.46±6.38) U in group F (all P0.05) at 6 hours after CLP.Meanwhile,the levels of MDA,XOD,ALT and hepatic water content in group C were all significantly higher than those in group A,but lower than those in the other four groups (all P0.05).The levels of MDA,XOD,ALT and hepatic water content in group E and group F were all significantly higher than those in group D (all P0.05),while their hepatic blood flow were lower than that in group D (both P0.05),and no significant differences were found in all the indices mentioned above between group E and group F (all P0.05).Conclusion Electroacupuncture at "Zúsānlǐ" (足三里 ST 36) can improve hepatic ischemia,inhibit lipid peroxidation and alleviate hepatic edema and dysfunction in septic rats,which might be related with the completeness of vagus nerves.展开更多
基金Supported by State Natural Sciences Foundation(NCFS):30672725Army Medical Health"11th Five-Year Plan"Special Subject:06Z055
文摘Objective To investigate protective effects of electroacupuncture on injury of lipid peroxidation-induced by liver ischemia in rats with sepsis.Methods Forty-eight male SD rats were subjected to sepsis by cecal ligation and puncture (CLP),and were randomly divided into a sham operation group (group A),a CLP model group (group B),a CLP model plus electroacupuncture (EA) at Zúsānlǐ (足三里 ST 36) group (group C),a CLP model plus electroacupuncture at a sham acupoint group (group D),a CLP model with vagotomy group (group E) and a CLP model plus electroacupuncture group after vagotomy (group F),with 8 rats in each group.CLP was performed after abdominal vagotomy in group E and group F.EA (2 mA,2/100 Hz) was applied at bilateral "Zúsānlǐ" (足三里 ST 36) in group C and group F,and at sham acupoints in group D,respectively,for 1 hour.The hepatic blood flow (HBF) was detected by a laser-doppler flowmetry at 6 hours after CLP.The activity of plasma alanine transarninase (ALT) was determined;contents of hepatic malondialdehyde (MDA),activity of xanthine oxidase (XOD) and the rate of water content in the hepati tissue were assessed,respectively.Results Hepatic blood flow was (56.97±11.95) U in group C,which was significantly lower than (80.12± 19.57) U shown in group A,but higher than (42.61±10.97) U in group B,(44.53±9.23) U in group D,(30.05 ±4.46) U in group E and (30.46±6.38) U in group F (all P0.05) at 6 hours after CLP.Meanwhile,the levels of MDA,XOD,ALT and hepatic water content in group C were all significantly higher than those in group A,but lower than those in the other four groups (all P0.05).The levels of MDA,XOD,ALT and hepatic water content in group E and group F were all significantly higher than those in group D (all P0.05),while their hepatic blood flow were lower than that in group D (both P0.05),and no significant differences were found in all the indices mentioned above between group E and group F (all P0.05).Conclusion Electroacupuncture at "Zúsānlǐ" (足三里 ST 36) can improve hepatic ischemia,inhibit lipid peroxidation and alleviate hepatic edema and dysfunction in septic rats,which might be related with the completeness of vagus nerves.