Generally, super Brownian motion will not c on verge vaguely to 0 if the initial measure is sufficiet large, so it is very inte resting to get asymptotic estimation for super Brownian motion. In this paper, w e will p...Generally, super Brownian motion will not c on verge vaguely to 0 if the initial measure is sufficiet large, so it is very inte resting to get asymptotic estimation for super Brownian motion. In this paper, w e will prove two asymptotic for super Brownian motion with general critical bran ching mechanism.展开更多
AEM: To investigate the expression pattern of epithelial cell adhesion molecule (Ep-CAM) on normal and malignant colon tissues to evaluate its diagnostic and therapeutic significance. METHODS: cDNA encoding Ep-CAM ext...AEM: To investigate the expression pattern of epithelial cell adhesion molecule (Ep-CAM) on normal and malignant colon tissues to evaluate its diagnostic and therapeutic significance. METHODS: cDNA encoding Ep-CAM extracellular domain was doned by reverse transcription-polymerase chain reaction (RT-PCR) from excised malignant colon tissues and inserted into a glutathione S-transferase (GST)-tagged vector. Ep-CAM-GST fusion protein was induced by isopropyl-p-D-thiogalactopyranoside (IPTG) and purified with glutathione-sepharose. The Ep-CAM-GST fusion protein was mixed with Freund's adjuvant and Balb/c mice were immunized with it. Sp2/0 myeloma cells were fused with the spleen cells of the immunized mice. After having selected by indirect ELISA, the anti-Ep-CAM monoclonal antibodies (MAbs) were generaled and the corresponding ascites were obtained. Finally, the human colon carcinoma tissue array prepared from seventy individual patients was stained with the anti-Ep-CAM MAbs. RESULTS: The isdated Ep-CAM cDNA sequence was identical to the data in GenBank. The expressed fusion protein was almost soluble and had a molecular weight (MW) of 53 ku. Four MAbs against Ep-CAM were obtained and designated as FMU-Epl, FMU-Ep2, FMU-Ep3 and FMU-Ep4 respectively. Among them, FMU-Ep4 could recognize the natural Ep-CAM on Colo205 and SW480 cells, and all of them could be used for immunohistochemical staining of tissue sections. It was fbund that Ep-CAM was distributed differently in normal and various malignant colon tissues, induding squamous cell carcinoma, signet-ring cell carcinoma and adenocarcinoma. In normal colon gland epithelia, Ep-CAM antigen was mainly distributed on the basolateral membrane and in the region between the basolateral membrane and the cytoplastic part near the nuclei, whereas the expression pattern of colon malignancies was mainly on the whole surface of epithelia and the expression was much higher than the normal colon tissues. The staining pattern of tissue array showed in adenocarcinoma and papillary adenocarcinoma, and the expression of Ep-CAM was increased from grade I to grade Ⅲ. CONCLUSION: MAbs against Ep-CAM might be useful for research on the structure and function of Ep-CAM and may have diagnostic and therapeutic value to various colon carcinomas.展开更多
It is imperative to evaluate factor of safety against basal heave failure in the design of braced deep excavation in soft clay.Based on previously published field monitoring data and finite element analyses of ground ...It is imperative to evaluate factor of safety against basal heave failure in the design of braced deep excavation in soft clay.Based on previously published field monitoring data and finite element analyses of ground settlements of deep excavation in soft clay,an assumed plastic deformation mechanism proposed here gives upper bound solutions for base stability of braced deep excavations.The proposed kinematic mechanism is optimized by the mobile depth(profile wavelength).The method takes into account the influence of strength anisotropy under plane strain conditions,the embedment of the retaining wall,and the locations of the struts.The current method is validated by comparison with published numerical study of braced excavations in Boston blue clay and two other cases of excavation failure in Taipei.The results show that the upper bound solutions obtained from this presented method is more accurate as compared with the conventional methods for basal heave failure analyses.展开更多
文摘Generally, super Brownian motion will not c on verge vaguely to 0 if the initial measure is sufficiet large, so it is very inte resting to get asymptotic estimation for super Brownian motion. In this paper, w e will prove two asymptotic for super Brownian motion with general critical bran ching mechanism.
基金Supported by the National Key Basic Research Special Funds (NKBRSF), No. 2001CB510004
文摘AEM: To investigate the expression pattern of epithelial cell adhesion molecule (Ep-CAM) on normal and malignant colon tissues to evaluate its diagnostic and therapeutic significance. METHODS: cDNA encoding Ep-CAM extracellular domain was doned by reverse transcription-polymerase chain reaction (RT-PCR) from excised malignant colon tissues and inserted into a glutathione S-transferase (GST)-tagged vector. Ep-CAM-GST fusion protein was induced by isopropyl-p-D-thiogalactopyranoside (IPTG) and purified with glutathione-sepharose. The Ep-CAM-GST fusion protein was mixed with Freund's adjuvant and Balb/c mice were immunized with it. Sp2/0 myeloma cells were fused with the spleen cells of the immunized mice. After having selected by indirect ELISA, the anti-Ep-CAM monoclonal antibodies (MAbs) were generaled and the corresponding ascites were obtained. Finally, the human colon carcinoma tissue array prepared from seventy individual patients was stained with the anti-Ep-CAM MAbs. RESULTS: The isdated Ep-CAM cDNA sequence was identical to the data in GenBank. The expressed fusion protein was almost soluble and had a molecular weight (MW) of 53 ku. Four MAbs against Ep-CAM were obtained and designated as FMU-Epl, FMU-Ep2, FMU-Ep3 and FMU-Ep4 respectively. Among them, FMU-Ep4 could recognize the natural Ep-CAM on Colo205 and SW480 cells, and all of them could be used for immunohistochemical staining of tissue sections. It was fbund that Ep-CAM was distributed differently in normal and various malignant colon tissues, induding squamous cell carcinoma, signet-ring cell carcinoma and adenocarcinoma. In normal colon gland epithelia, Ep-CAM antigen was mainly distributed on the basolateral membrane and in the region between the basolateral membrane and the cytoplastic part near the nuclei, whereas the expression pattern of colon malignancies was mainly on the whole surface of epithelia and the expression was much higher than the normal colon tissues. The staining pattern of tissue array showed in adenocarcinoma and papillary adenocarcinoma, and the expression of Ep-CAM was increased from grade I to grade Ⅲ. CONCLUSION: MAbs against Ep-CAM might be useful for research on the structure and function of Ep-CAM and may have diagnostic and therapeutic value to various colon carcinomas.
基金supported by the National Science Foundation for Distinguished Young Scholars of China(Grant No.51325901)the State Key Program of National Natural Science of China(Grant No.51338009)
文摘It is imperative to evaluate factor of safety against basal heave failure in the design of braced deep excavation in soft clay.Based on previously published field monitoring data and finite element analyses of ground settlements of deep excavation in soft clay,an assumed plastic deformation mechanism proposed here gives upper bound solutions for base stability of braced deep excavations.The proposed kinematic mechanism is optimized by the mobile depth(profile wavelength).The method takes into account the influence of strength anisotropy under plane strain conditions,the embedment of the retaining wall,and the locations of the struts.The current method is validated by comparison with published numerical study of braced excavations in Boston blue clay and two other cases of excavation failure in Taipei.The results show that the upper bound solutions obtained from this presented method is more accurate as compared with the conventional methods for basal heave failure analyses.
