[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in whic...[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in which BEH C18 column （2.1 mmx50 ram, 1,7 urn） was used with a mobile phase of methanol-water at a flow rate was of 0.3 ml/min, and the column temperature was of 30 ℃; and an evaporative light-scattering detector was used, the temperature in drift tube was of 90 ℃ and the pressure of N2 was of 20 psi. [Concludoa] This research provided theoretical foundation for the determination of β-sitosterol in Ethnodrug Guoshangye.展开更多
AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to ...AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.展开更多
Based upon a case study of Silver Beach coastal resort of Beihai, Guangxi Zhuang Autonomous Region, this article examines the rise and fall of Chinese coastal tourist resort development since the 1980s. It explores th...Based upon a case study of Silver Beach coastal resort of Beihai, Guangxi Zhuang Autonomous Region, this article examines the rise and fall of Chinese coastal tourist resort development since the 1980s. It explores the causes for and responses to the decline of Chinese coastal resorts. The data were collected through in-depth interviews with twelve local key information providers, and secondary information sources such as government archives, survey reports, and resort plans. It was found that the rise and fall of Chinese coastal resorts in the last three decades is closely linked to the transitional reform from a central planned economy to a market economy. Three stages of coastal tourism development were identified in a sequence of rapid growth, decline and redevelopment. It was government financial funds and high local government involvement that promoted the rapid expansion of coastal resorts during the 1980s. However, coastal resort decline occurred when transitional reform was intensified and deflationary policy executed af- ter 1992. Institutional failure is found to be the main reason of Chinese coastal resorts decline since the mid-1990s. The government-dominated pattern could not be sustained and should be restructured in forthcoming coastal tourism de- velopment in China. Revival strategies should emphasize enhancing market-oriented reform in the declining resorts on both suoolv and demand sides.展开更多
Recent liberal political science analysis has highlighted media, manipu- lation, and populist political trickery in the apparently sudden rise of the new Right in Europe and the USA. I suggest that a robust engagement...Recent liberal political science analysis has highlighted media, manipu- lation, and populist political trickery in the apparently sudden rise of the new Right in Europe and the USA. I suggest that a robust engagement with the actual social transformations over which liberalism has presided since 1989 is imperative. Anthropological work on class processes and the rise of neo-nationalist populism in Central and Eastern Europe has been strong in developing a more relational, pro- cessual, and embedded vision. In the current paper, I am looking at the phases and spaces of the rise of iUiberalism as a popular political sensibility in Central and Eastern Europe. In particular, I am interested in its gradual upscaling to the level of the nation state and, through the ＂Visegrad bloc＂ to the EU. I argue that both the emergence and step-by-step upscaling of illiberal political sensibilities are explained by class relational processes and the regionally uneven Polanyi-type ＂counter- movements＂ against liberalizations that they brought forth.展开更多
基金Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technique Research Subject of Guizhou Administrative Bureau of TCM(QZYY2012-34)贵州省中医药管理局中医药,民族医药科学技术研究课题(QZYY2012-34)
文摘[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in which BEH C18 column （2.1 mmx50 ram, 1,7 urn） was used with a mobile phase of methanol-water at a flow rate was of 0.3 ml/min, and the column temperature was of 30 ℃; and an evaporative light-scattering detector was used, the temperature in drift tube was of 90 ℃ and the pressure of N2 was of 20 psi. [Concludoa] This research provided theoretical foundation for the determination of β-sitosterol in Ethnodrug Guoshangye.
基金Supported by National Health and Medical Research Council, Australia
文摘AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.
基金Under the auspices of National Natural Science Foundation of China (No. 70703012)Cutural and Social Foundation of National Education Ministry (No.11YJAZH059)
文摘Based upon a case study of Silver Beach coastal resort of Beihai, Guangxi Zhuang Autonomous Region, this article examines the rise and fall of Chinese coastal tourist resort development since the 1980s. It explores the causes for and responses to the decline of Chinese coastal resorts. The data were collected through in-depth interviews with twelve local key information providers, and secondary information sources such as government archives, survey reports, and resort plans. It was found that the rise and fall of Chinese coastal resorts in the last three decades is closely linked to the transitional reform from a central planned economy to a market economy. Three stages of coastal tourism development were identified in a sequence of rapid growth, decline and redevelopment. It was government financial funds and high local government involvement that promoted the rapid expansion of coastal resorts during the 1980s. However, coastal resort decline occurred when transitional reform was intensified and deflationary policy executed af- ter 1992. Institutional failure is found to be the main reason of Chinese coastal resorts decline since the mid-1990s. The government-dominated pattern could not be sustained and should be restructured in forthcoming coastal tourism de- velopment in China. Revival strategies should emphasize enhancing market-oriented reform in the declining resorts on both suoolv and demand sides.
文摘Recent liberal political science analysis has highlighted media, manipu- lation, and populist political trickery in the apparently sudden rise of the new Right in Europe and the USA. I suggest that a robust engagement with the actual social transformations over which liberalism has presided since 1989 is imperative. Anthropological work on class processes and the rise of neo-nationalist populism in Central and Eastern Europe has been strong in developing a more relational, pro- cessual, and embedded vision. In the current paper, I am looking at the phases and spaces of the rise of iUiberalism as a popular political sensibility in Central and Eastern Europe. In particular, I am interested in its gradual upscaling to the level of the nation state and, through the ＂Visegrad bloc＂ to the EU. I argue that both the emergence and step-by-step upscaling of illiberal political sensibilities are explained by class relational processes and the regionally uneven Polanyi-type ＂counter- movements＂ against liberalizations that they brought forth.
