Background: DNA of epidermodysplasia verruciformis (EV)-associated human papillomaviruses (HPVs) has been widely detected in lesions of malignant skin tumours, benign tumours and other proliferative diseases of epithe...Background: DNA of epidermodysplasia verruciformis (EV)-associated human papillomaviruses (HPVs) has been widely detected in lesions of malignant skin tumours, benign tumours and other proliferative diseases of epithelial origin. Objectives: To investigate the presence of EV-associated HPV DNA in nongenital seborrhoeic keratosis (SK) and to elucidate the prevalence of distinct HPV genotypes. Methods: We investigated HPV DNA in 55 nongenital SK biopsies, which were compared with 48 normal skin biopsies (healthy controls) using a nested polymerase chain reaction (PCR) using consensus primers CP65/CP70 and CP66/CP69. The positive PCR products were retracted and used to prepare recombination clones with T-vector. Distinct clones were analysed with endonucleases, and HPV genotypes were identified by direct sequencing. Results: EV-associated HPV DNA was detected in 42 of 55 (76%) nongenital SK biopsies vs. only 13 of 48 (27%) healthy controls (χ2=22.087; P < 0.005). The prevalencewas higher in patients with more than five lesions than in those with only one lesion (P < 0.05). Ten distinct HPV genotypes were detected in the nongenital SK biopsies: HPV 20, 23, 5, renal transplant recipient (RTR) X7, HPV 17, 37, 17b, RTRX4, RTRX4b and strain SK3. HPV 20 was found in 26 of 42 (62%) positive specimens, followed by HPV 23 (11 of 42, 26%) and HPV 5 (six of 42, 14%). Existence of multiple HPV genotypes was observed in 12 of 42 (29%) positive specimens. In healthy controls, five genotypes of EV-associated HPV (HPV 20, 23, 5, 17 and RTRX4) were detected, with the same predominant genotype of HPV 20 (five of 13, 38%). Several distinct HPV genotypes were found to coexist in four of 13 (31%) positive specimens. Conclusions: This study provides some evidence that EV-associated HPVs might play a part in the pathogenesis of nongenital SK.展开更多
长骨造釉细胞瘤（Adamantinoma of the long bones，LAD），简称造釉细胞瘤（AD），是一种罕见的低度恶性的原发性骨肿瘤，其发生率约为原发性骨肿瘤的0．1％～0．5％。AD可发生于全身长管状骨，但好发部位为胫骨中段或中下段前缘，伴...长骨造釉细胞瘤（Adamantinoma of the long bones，LAD），简称造釉细胞瘤（AD），是一种罕见的低度恶性的原发性骨肿瘤，其发生率约为原发性骨肿瘤的0．1％～0．5％。AD可发生于全身长管状骨，但好发部位为胫骨中段或中下段前缘，伴或不伴同侧腓骨病变。因AD罕见且临床特点不典型，临床上常与骨性纤维结构不良（osteofibrous dysplasia，0FD）等良恶性骨病变难以鉴别，展开更多
文摘Background: DNA of epidermodysplasia verruciformis (EV)-associated human papillomaviruses (HPVs) has been widely detected in lesions of malignant skin tumours, benign tumours and other proliferative diseases of epithelial origin. Objectives: To investigate the presence of EV-associated HPV DNA in nongenital seborrhoeic keratosis (SK) and to elucidate the prevalence of distinct HPV genotypes. Methods: We investigated HPV DNA in 55 nongenital SK biopsies, which were compared with 48 normal skin biopsies (healthy controls) using a nested polymerase chain reaction (PCR) using consensus primers CP65/CP70 and CP66/CP69. The positive PCR products were retracted and used to prepare recombination clones with T-vector. Distinct clones were analysed with endonucleases, and HPV genotypes were identified by direct sequencing. Results: EV-associated HPV DNA was detected in 42 of 55 (76%) nongenital SK biopsies vs. only 13 of 48 (27%) healthy controls (χ2=22.087; P < 0.005). The prevalencewas higher in patients with more than five lesions than in those with only one lesion (P < 0.05). Ten distinct HPV genotypes were detected in the nongenital SK biopsies: HPV 20, 23, 5, renal transplant recipient (RTR) X7, HPV 17, 37, 17b, RTRX4, RTRX4b and strain SK3. HPV 20 was found in 26 of 42 (62%) positive specimens, followed by HPV 23 (11 of 42, 26%) and HPV 5 (six of 42, 14%). Existence of multiple HPV genotypes was observed in 12 of 42 (29%) positive specimens. In healthy controls, five genotypes of EV-associated HPV (HPV 20, 23, 5, 17 and RTRX4) were detected, with the same predominant genotype of HPV 20 (five of 13, 38%). Several distinct HPV genotypes were found to coexist in four of 13 (31%) positive specimens. Conclusions: This study provides some evidence that EV-associated HPVs might play a part in the pathogenesis of nongenital SK.
文摘长骨造釉细胞瘤（Adamantinoma of the long bones，LAD），简称造釉细胞瘤（AD），是一种罕见的低度恶性的原发性骨肿瘤，其发生率约为原发性骨肿瘤的0．1％～0．5％。AD可发生于全身长管状骨，但好发部位为胫骨中段或中下段前缘，伴或不伴同侧腓骨病变。因AD罕见且临床特点不典型，临床上常与骨性纤维结构不良（osteofibrous dysplasia，0FD）等良恶性骨病变难以鉴别，
