OBJECTIVE To study the expression levels of Twist and epithelialmesenchymal transitions in multidrug-resistant MCF-7/ADR breast cancer cells, and to study the relationship between multidrug resistance （MDR） and meta...OBJECTIVE To study the expression levels of Twist and epithelialmesenchymal transitions in multidrug-resistant MCF-7/ADR breast cancer cells, and to study the relationship between multidrug resistance （MDR） and metastatic potential of the cells. METHODS RT-PCR, immunohistochemical and Western blotting methods were used to examine the changes of expression levels of the transcription factor Twist, E-cadherin and N-cadherin in the MCF-7 breast cancer cell line and its multidrug-resistant variant, MCF-7/ADR. RESULTS In MCF-7 cells, the expression of E-cadherin can be detected, but there is no expression of Twist or N-cadherin. In MCF-7/ADR cells, E-cadherin expression is lost, but the expression of two other genes was significantly positive. CONCLUSION Epithelial-mesenchymal transitions induced by Twist, may have a relationship with enhanced invasion and metastatic potential during the development of multidrug-resistant MCF-7/ADR breast cancer cells.展开更多
文摘目的评估PSMD6在肝细胞癌(HCC)患者组织中的表达情况,并探究其潜在临床价值。方法基于Gene Expression Omnibus(GEO)、Metabric、TCGA-GTEx、ArrayExpress、Sequence Read Archive(SRA)数据库,综合分析全球多中心、多平台的PSMD6基因表达谱,探究PSMD6的mRNA在HCC中的过表达趋势。同时,使用The Human Protein Atlas组织芯片库展示PSMD6在HCC组织中蛋白表达情况。绘制KM曲线,分析PSMD6在HCC中的预后预测价值。结果PSMD6蛋白表达的阳性染色信号定位于HCC细胞的细胞浆和核膜中,表达强度中等,染色度中等。HCC中PSMD6高表达(P<0.01),结果未发生偏倚。SROC的AUC为0.75(0.71,0.79),敏感性为0.64,特异性为0.74,提示PSMD6具有中度鉴别HCC的能力。生存分析显示,PSMD6高表达的患者面临着更高的生存风险(HR=1.4,P=0.043)。结论PSMD6在HCC中异常高表达,可作为HCC筛查和治疗潜在靶点。
基金the grants from National Natural Science Foundation (No.30370553) of China Tianjin Medi-cal University Natural Science Foundation (No.2005KY41).
文摘OBJECTIVE To study the expression levels of Twist and epithelialmesenchymal transitions in multidrug-resistant MCF-7/ADR breast cancer cells, and to study the relationship between multidrug resistance （MDR） and metastatic potential of the cells. METHODS RT-PCR, immunohistochemical and Western blotting methods were used to examine the changes of expression levels of the transcription factor Twist, E-cadherin and N-cadherin in the MCF-7 breast cancer cell line and its multidrug-resistant variant, MCF-7/ADR. RESULTS In MCF-7 cells, the expression of E-cadherin can be detected, but there is no expression of Twist or N-cadherin. In MCF-7/ADR cells, E-cadherin expression is lost, but the expression of two other genes was significantly positive. CONCLUSION Epithelial-mesenchymal transitions induced by Twist, may have a relationship with enhanced invasion and metastatic potential during the development of multidrug-resistant MCF-7/ADR breast cancer cells.
