The fabrication of Bragg gratings on silicon-on-insulator (SOI) rib waveguides using electron-beam lithography is presented. The grating waveguide is optimally designed for actual photonic integration. Experimental ...The fabrication of Bragg gratings on silicon-on-insulator (SOI) rib waveguides using electron-beam lithography is presented. The grating waveguide is optimally designed for actual photonic integration. Experimental and theoretical evaluations of the Bragg grating are demonstrated. By thinning the SOl device layer and deeply etching the Bragg grating, a large grating coupling coefficient of 30cm^-1 is obtained.展开更多
Grading procedure in routine sea cucumber hatchery production is thought to affect juvenile sea cucumber immunologi- cal response. The present study investigated the impact of a 3-min mechanical perturbation mimicking...Grading procedure in routine sea cucumber hatchery production is thought to affect juvenile sea cucumber immunologi- cal response. The present study investigated the impact of a 3-min mechanical perturbation mimicking the grading procedure on neuroendocrine and immune parameters of the sea cucumber Apostichopusjaponicus. During the application of stress, concentrations of noradrenaline and dopamine in coelomic fluid increased significantly, indicating that the mechanical perturbation resulted in a transient state of stress in sea cucumbers. Coelomocytes concentration in coelomic fluid increased transiently after the beginning of stressing, and reached the maximum in 1 h. Whereas, coelomocytes phagocytosis at 3 min, superoxide anion production from 3 min to 0.5 h, acid phosphatase activity at 0.5 h, and phenoloxidase activity from 3 min to 0.5 h were all significantly down-regulated. All of the immune parameters recovered to baseline levels after the experiment was conducted for 8 h, and an immunostimulation occurred after the stress considering the phagocytosis and acid phosphatase activity. The results suggested that, as in other marine invertebrates, neuroendocrine/immnne connections exist in sea cucumber A. japonicus. Mechanical stress can elicit a profound influence on sea cucumber neuroendocrine system. Neuroendocrine messengers act in turn to modulate the immunity fimctions. Therefore, these ef- fects should be considered for developing better husbandry procedures.展开更多
Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neu...Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neurogenic bladder after suprasacral spinal cord injury, and to preliminarily explore its partial mechanism of action. Methods Forty-eight female SD rats were randomly divided into blank group(group B), sham operation group(group S), model group(group M) and electroacupuncture group(group EA) according to random number table method with 12 rats in each group. Neurogenic bladder models after suprasacral spinal cord injury were established by adopting modified spinal cord transection method. Electroacupuncture intervention was conducted at "Cìliáo(次髎 BL 32)""Zhōngjí(中极 CV 3)" "Sānyīnjiāo(三阴交 SP 6)" and "Dàzhuī(大椎 GV 14)" on the 19 th day after modeling. After treatment for 7 consecutive days, the rats were sacrificed, and the bladder tissues were removed rapidly for microscopic observation of morphological change after HE stain and for determination of the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 via Western blot method. Results① Under light microscope after HE stain, injury in varying degrees in muscle fiber arrangement and the shape and size of cells, etc. of bladder detrusor of rats in group M and group EA was found, and the degree of injury of detrusor in group M was severer than that in group EA;② comparison of the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 between group M as well as group EA and group B as well as group S: the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group M and group EA obviously increased( P〈0.01, P〈0.05); comparison between group EA and group M: the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group EA obviously reduced( P〈0.05);③ comparison of the protein expression level of Caspase-3 between group M as well as group EA and group B as well as group S: the protein expression level of Pro-Caspase-3(35 KD) in group M and group EA reduced, and the protein expression level of activated Caspase-3(17 KD/19 KD) obviously increased( P〈0.01, P〈0.05); comparison between group EA andgroup M: the protein expression level of Pro-Caspase-3(35 KD) in group EA obviously increased, and the protein expression level of activated Caspase-3(17 KD/19 KD) obviously reduced( P〈0.05). Conclusion suprasacral spinal cord injury can increase the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats, and electroacupuncture at BL 32, CV 3, SP 6 and GV 14 can down-regulate the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats after suprasacral spinal cord injury and protect bladder tissue, which may be one of the mechanisms that electroacupuncture protects the involved bladder detrusor after suprasacral spinal cord injury.展开更多
Objective: To investigate the effect and mechanism of electroacupuncture(EA) in the intervention of neurogenic bladder(NB) caused by suprasacral spinal cord injury(SCI).Methods: The total 60 female Sprague-Daw...Objective: To investigate the effect and mechanism of electroacupuncture(EA) in the intervention of neurogenic bladder(NB) caused by suprasacral spinal cord injury(SCI).Methods: The total 60 female Sprague-Dawley(SD) rats were divided into blank group, sham operation group by adopting random number table method, with 12 rats in each group. The remaining rats were made into models through operation, and 24 successful model rats were randomly divided into model group and EA group, with 12 rats in each group. Since the 19 th day after modeling, EA was conducted at "Cìliào"(次髎BL 32), "Zhōngjí"(中极CV 3),"Sānyīnjiāo"(三阴交SP 6) and "Dàzhuī"(大椎GV 14) in EA group, remaining the needle for 20 min every time. The intervention was performed for once a day, and7 consecutive days were needed. The rest of the three groups were only tied up for the same time. Urodynamics test was conducted after the intervention. The contents of PACAP-38 and PAC1 R of the spinal cord of the injury site were detected by Western blot.Results: Compared with the blank group. there were no significant differences in the bladder capacity,leak point pressure, bladder compliance and PACAP-38 and PAC1 R contents in spinal cord in sham operation group; compared with the sham operation group, the maximum bladder capacity and the bladder compliance in the model group were lower(both P〈0.05), the leak point pressure was higher(P〈0.05),the contents of PACAP-38 and PAC1 R were lower(both P〈0.05); compared with the model group, the maximum bladder capacity and the bladder compliance in the EA group were higher(both P〈0.05), the leak point pressure was lower(P〈0.05), the contents of PACAP-38 and PAC1 R were higher(both P〈0.05).Conclusion: EA at BL 32, CV 3, SP 6 and GV 14 can improve the bladder function of NB rats of suprasacral SCI, and its mechanism may be related to the increase expression of PACAP-38 and PAC1 R protein in the injured spinal cord tissue.展开更多
文摘The fabrication of Bragg gratings on silicon-on-insulator (SOI) rib waveguides using electron-beam lithography is presented. The grating waveguide is optimally designed for actual photonic integration. Experimental and theoretical evaluations of the Bragg grating are demonstrated. By thinning the SOl device layer and deeply etching the Bragg grating, a large grating coupling coefficient of 30cm^-1 is obtained.
基金financially supported by the 863 High Technology Project of the Ministry of Science and Technology (No. 2012AA10A412-4)the Special Funds for the Basic B & D Program in the Central Non-profit Research Institutes (No. 2010-cb-03)+1 种基金Science and Technology Development Planning Project of Shandong Province (No. 2012GGA06021)Science and Technology Development Fund of Shinan district of Qingdao (No. 2011-5-023-QT)
文摘Grading procedure in routine sea cucumber hatchery production is thought to affect juvenile sea cucumber immunologi- cal response. The present study investigated the impact of a 3-min mechanical perturbation mimicking the grading procedure on neuroendocrine and immune parameters of the sea cucumber Apostichopusjaponicus. During the application of stress, concentrations of noradrenaline and dopamine in coelomic fluid increased significantly, indicating that the mechanical perturbation resulted in a transient state of stress in sea cucumbers. Coelomocytes concentration in coelomic fluid increased transiently after the beginning of stressing, and reached the maximum in 1 h. Whereas, coelomocytes phagocytosis at 3 min, superoxide anion production from 3 min to 0.5 h, acid phosphatase activity at 0.5 h, and phenoloxidase activity from 3 min to 0.5 h were all significantly down-regulated. All of the immune parameters recovered to baseline levels after the experiment was conducted for 8 h, and an immunostimulation occurred after the stress considering the phagocytosis and acid phosphatase activity. The results suggested that, as in other marine invertebrates, neuroendocrine/immnne connections exist in sea cucumber A. japonicus. Mechanical stress can elicit a profound influence on sea cucumber neuroendocrine system. Neuroendocrine messengers act in turn to modulate the immunity fimctions. Therefore, these ef- fects should be considered for developing better husbandry procedures.
基金Supported by National Natural Science Foundation of China:81473753
文摘Objective To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neurogenic bladder after suprasacral spinal cord injury, and to preliminarily explore its partial mechanism of action. Methods Forty-eight female SD rats were randomly divided into blank group(group B), sham operation group(group S), model group(group M) and electroacupuncture group(group EA) according to random number table method with 12 rats in each group. Neurogenic bladder models after suprasacral spinal cord injury were established by adopting modified spinal cord transection method. Electroacupuncture intervention was conducted at "Cìliáo(次髎 BL 32)""Zhōngjí(中极 CV 3)" "Sānyīnjiāo(三阴交 SP 6)" and "Dàzhuī(大椎 GV 14)" on the 19 th day after modeling. After treatment for 7 consecutive days, the rats were sacrificed, and the bladder tissues were removed rapidly for microscopic observation of morphological change after HE stain and for determination of the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 via Western blot method. Results① Under light microscope after HE stain, injury in varying degrees in muscle fiber arrangement and the shape and size of cells, etc. of bladder detrusor of rats in group M and group EA was found, and the degree of injury of detrusor in group M was severer than that in group EA;② comparison of the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 between group M as well as group EA and group B as well as group S: the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group M and group EA obviously increased( P〈0.01, P〈0.05); comparison between group EA and group M: the protein expression levels of cytochrome C, Pro-Caspase-9 and activated Caspase-9 in bladder tissue of rats in group EA obviously reduced( P〈0.05);③ comparison of the protein expression level of Caspase-3 between group M as well as group EA and group B as well as group S: the protein expression level of Pro-Caspase-3(35 KD) in group M and group EA reduced, and the protein expression level of activated Caspase-3(17 KD/19 KD) obviously increased( P〈0.01, P〈0.05); comparison between group EA andgroup M: the protein expression level of Pro-Caspase-3(35 KD) in group EA obviously increased, and the protein expression level of activated Caspase-3(17 KD/19 KD) obviously reduced( P〈0.05). Conclusion suprasacral spinal cord injury can increase the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats, and electroacupuncture at BL 32, CV 3, SP 6 and GV 14 can down-regulate the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder tissues of rats after suprasacral spinal cord injury and protect bladder tissue, which may be one of the mechanisms that electroacupuncture protects the involved bladder detrusor after suprasacral spinal cord injury.
文摘Objective: To investigate the effect and mechanism of electroacupuncture(EA) in the intervention of neurogenic bladder(NB) caused by suprasacral spinal cord injury(SCI).Methods: The total 60 female Sprague-Dawley(SD) rats were divided into blank group, sham operation group by adopting random number table method, with 12 rats in each group. The remaining rats were made into models through operation, and 24 successful model rats were randomly divided into model group and EA group, with 12 rats in each group. Since the 19 th day after modeling, EA was conducted at "Cìliào"(次髎BL 32), "Zhōngjí"(中极CV 3),"Sānyīnjiāo"(三阴交SP 6) and "Dàzhuī"(大椎GV 14) in EA group, remaining the needle for 20 min every time. The intervention was performed for once a day, and7 consecutive days were needed. The rest of the three groups were only tied up for the same time. Urodynamics test was conducted after the intervention. The contents of PACAP-38 and PAC1 R of the spinal cord of the injury site were detected by Western blot.Results: Compared with the blank group. there were no significant differences in the bladder capacity,leak point pressure, bladder compliance and PACAP-38 and PAC1 R contents in spinal cord in sham operation group; compared with the sham operation group, the maximum bladder capacity and the bladder compliance in the model group were lower(both P〈0.05), the leak point pressure was higher(P〈0.05),the contents of PACAP-38 and PAC1 R were lower(both P〈0.05); compared with the model group, the maximum bladder capacity and the bladder compliance in the EA group were higher(both P〈0.05), the leak point pressure was lower(P〈0.05), the contents of PACAP-38 and PAC1 R were higher(both P〈0.05).Conclusion: EA at BL 32, CV 3, SP 6 and GV 14 can improve the bladder function of NB rats of suprasacral SCI, and its mechanism may be related to the increase expression of PACAP-38 and PAC1 R protein in the injured spinal cord tissue.