[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in whic...[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in which BEH C18 column （2.1 mmx50 ram, 1,7 urn） was used with a mobile phase of methanol-water at a flow rate was of 0.3 ml/min, and the column temperature was of 30 ℃; and an evaporative light-scattering detector was used, the temperature in drift tube was of 90 ℃ and the pressure of N2 was of 20 psi. [Concludoa] This research provided theoretical foundation for the determination of β-sitosterol in Ethnodrug Guoshangye.展开更多
基金Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technique Research Subject of Guizhou Administrative Bureau of TCM(QZYY2012-34)贵州省中医药管理局中医药,民族医药科学技术研究课题(QZYY2012-34)
文摘[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in which BEH C18 column （2.1 mmx50 ram, 1,7 urn） was used with a mobile phase of methanol-water at a flow rate was of 0.3 ml/min, and the column temperature was of 30 ℃; and an evaporative light-scattering detector was used, the temperature in drift tube was of 90 ℃ and the pressure of N2 was of 20 psi. [Concludoa] This research provided theoretical foundation for the determination of β-sitosterol in Ethnodrug Guoshangye.
