Let ωα （α=1,…,n） be the holomorphic invariant forms introduced by the author previously ona bounded domain D in Cn for n ≥ 2. Set ωα=（i/2）α ωα.Then for any complex surface S in D we have ω2/1｜S≥ω2｜s.
The molecular scales behavior of interracial water at the solid/liquid interfaces is of a fundamental significance in a diverse set of technical and scientific contexts, ranging from the efficiency of oil mining to th...The molecular scales behavior of interracial water at the solid/liquid interfaces is of a fundamental significance in a diverse set of technical and scientific contexts, ranging from the efficiency of oil mining to the activity of biological molecules. Recently, it has become recognized that, both the physical interactions and the surface morphology have significant impact on the behavior of inter- facial water, including the water structures as well as the wetting properties of the surface. In this review, we summarize some of recent advances in the atom-level pictures of the interfacial water, which exhibits the ordered character on various solid surfaces at room or cryogenic temperature. Special focus has been devoted to the wetting phenomenon of ＂ordered water monolayer that does not completely wet water＂ and the underlying mechanism on model and some real solid surfaces at room temperature. The possible applications of this phenomenon are also discussed.展开更多
Human immunodeficiency virus-1 （HIV-1）encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between t...Human immunodeficiency virus-1 （HIV-1）encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between the HIV-1 proteins to better understand the role played by viral protein-protein interactions in the life cycle of HIV-I. Genes encoding the 15 viral proteins from the HIV-1 strain AD8 were inserted into the plasmids of a yeast two-hybrid system. By screening 120 pairs of proteins, interactions between seven pairs were found. This led to the discovery of an interaction between the HIV-1 proteins integrase （IN） and glycoprotein 41 （gp41）, which was confirmed by both co-immunoprecipitation （Co-IP） assays and fluorescence resonance energy transfer （FRET） imaging in live cells. In addition, it was found that the amino acids at positions 76-100 of gp41 are required for it to bind to IN. Deletion of this region from gp41 prevented its interaction with IN and reduced the production of HIV-1 in 293T cells. This study provides new information on HIV-1 protein-protein interactions which improves the understanding of the biological functions of gp41 and IN during the virus life cycle.展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.A01010501 and 10731080)
文摘Let ωα （α=1,…,n） be the holomorphic invariant forms introduced by the author previously ona bounded domain D in Cn for n ≥ 2. Set ωα=（i/2）α ωα.Then for any complex surface S in D we have ω2/1｜S≥ω2｜s.
基金supported by the National Natural Science Foundation of China (Nos. 11290164 and 11204341)the Knowledge Innovation Program of SINAP+1 种基金the Knowledge Innovation Program of the Chinese Academy of Sciences,Shanghai Supercomputer Center of ChinaSupercomputing Center of Chinese Academy of Sciences
文摘The molecular scales behavior of interracial water at the solid/liquid interfaces is of a fundamental significance in a diverse set of technical and scientific contexts, ranging from the efficiency of oil mining to the activity of biological molecules. Recently, it has become recognized that, both the physical interactions and the surface morphology have significant impact on the behavior of inter- facial water, including the water structures as well as the wetting properties of the surface. In this review, we summarize some of recent advances in the atom-level pictures of the interfacial water, which exhibits the ordered character on various solid surfaces at room or cryogenic temperature. Special focus has been devoted to the wetting phenomenon of ＂ordered water monolayer that does not completely wet water＂ and the underlying mechanism on model and some real solid surfaces at room temperature. The possible applications of this phenomenon are also discussed.
基金supported by the National Nano Project(grant no.2011CB933600)the National Natural Science Foundation of China(grant no.31470269)supported by the Institute of Biophysics and the National Laboratory of Biomacromolecules at the Chinese Academy of Sciences(CAS)
文摘Human immunodeficiency virus-1 （HIV-1）encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between the HIV-1 proteins to better understand the role played by viral protein-protein interactions in the life cycle of HIV-I. Genes encoding the 15 viral proteins from the HIV-1 strain AD8 were inserted into the plasmids of a yeast two-hybrid system. By screening 120 pairs of proteins, interactions between seven pairs were found. This led to the discovery of an interaction between the HIV-1 proteins integrase （IN） and glycoprotein 41 （gp41）, which was confirmed by both co-immunoprecipitation （Co-IP） assays and fluorescence resonance energy transfer （FRET） imaging in live cells. In addition, it was found that the amino acids at positions 76-100 of gp41 are required for it to bind to IN. Deletion of this region from gp41 prevented its interaction with IN and reduced the production of HIV-1 in 293T cells. This study provides new information on HIV-1 protein-protein interactions which improves the understanding of the biological functions of gp41 and IN during the virus life cycle.
