An in vitro shoot regeneration procedure was developed in pepper ( Capsicum annuum L. ) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from co...An in vitro shoot regeneration procedure was developed in pepper ( Capsicum annuum L. ) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates ( respectively 100.0% and 58.3% ) and average number of adventitious bud from each explant (respectively 18.8 and 13.2) were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be: MS + 3.0 mg/L 6-BA + 1.0 mg/L IAA + 5.0 mg/L AgNO3 + 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.展开更多
In this study we used U0126, a potent and specific inhibitor of MEK, to study the roles of MEK/ERK/p90rsk signaling pathway in the meiotic cell cycle of mouse oocytes. The phosphorylation of MAP kinase and p90rsk in t...In this study we used U0126, a potent and specific inhibitor of MEK, to study the roles of MEK/ERK/p90rsk signaling pathway in the meiotic cell cycle of mouse oocytes. The phosphorylation of MAP kinase and p90rsk in the oocytes treated with 1.5 μMU0126 was the same as that in oocytes cultured in drug-free medium. With 1.5 μM U0126 treatment, the spindles appeared normal as they formed in oocytes, but failed to maintain its structure.Instead, the spindle lost one pole or elongated extraordinarily. After further culture, some oocytes extruded gigantic polar bodies (>30 μm) that later divided into two small ones. Some oocytes underwent symmetric division and produced two equal-size daughter cells in which normal spindles formed. In oocytes with different division patterns,MAP kinase was normally phosphorylated. When the concentration of U0126 was increased to 15 mM, the phosphorylation of both MAPK and p90rsk were inhibited, while symmetric division was decreased. When incubating in medium containing 15 μM U0126 for 14 h, oocytes were activated, but part of them failed to emit polar bodies. MII oocytes were also activated by 15 μM U0126, at the same time the dephosphorylation of MAP kinase and p90rsk was observed. Our results indicate that 1) MEK plays important but not indispensable roles in microtubule organization;2) MEK keeps normal meiotic spindle morphology, targets peripheral spindle positioning and regulates asymmetric division by activating some unknown substrates other than MAP kinase/p90rsk; and 3) activation of MEK/ERK/p90rsk cascade maintains MII arrest in mouse oocytes.展开更多
In this paper we propose a tripartite scheme for splitting an arbitrary 2-qubit quantum information by using two asymmetric W states as the quantum channel. In the schemem if the two recipients collaborate together, t...In this paper we propose a tripartite scheme for splitting an arbitrary 2-qubit quantum information by using two asymmetric W states as the quantum channel. In the schemem if the two recipients collaborate together, they can deterministically recover the quantum information by performing first a 4-qubit collective unitary operation and then two single-qubit unitary operations. In addition, since the asymmetric W states are employed as the quantum channel, the scheme is robust against decoherence.展开更多
The asymmetric breakups of a droplet in an axisymmetric cross-like microfluidic device are investigated by using a three-dimensional volume of fluid(VOF) multiphase numerical model. Two kinds of asymmetries(droplet lo...The asymmetric breakups of a droplet in an axisymmetric cross-like microfluidic device are investigated by using a three-dimensional volume of fluid(VOF) multiphase numerical model. Two kinds of asymmetries(droplet location deviation from the symmetric geometry center and different flow rates at two symmetric outlets) generate asymmetric flow fields near the droplet, which results in the asymmetric breakup of the latter. Four typical breakup regimes(no breakup, one-side breakup, retraction breakup and direct breakup) have been observed.Two regime maps are plotted to describe the transition from one regime to another for the two types of different asymmetries, respectively. A power law model, which is based on the three critical factors(the capillary number,the asymmetry of flow fields and the initial volume ratio), is employed to predict the volume ratio of the two unequal daughter droplets generated in the direct breakup. The influences of capillary numbers and the asymmetries have been studied systematically in this paper. The larger the asymmetry is, the bigger the oneside breakup zone is. The larger the capillary number is, the more possible the breakup is in the direct breakup zone. When the radius of the initial droplet is 20 μm, the critical capillary numbers are 0.122, 0.128, 0.145,0.165, 0.192 and 0.226 for flow asymmetry factor AS= 0.05, 0.1, 0.2, 0.3, 0.4 and 0.5, respectively, in the flow system whose asymmetry is generated by location deviations. In the flow system whose asymmetry is generated by two different flow rates at two outlets, the critical capillary numbers are 0.121, 0.133, 0.145, 0.156 and 0.167 for AS= 1/21, 3/23, 1/5, 7/27 and 9/29, respectively.展开更多
The space block search technology is used to determine a connected three-dimensional fracture network in polygonal shapes,i.e.,seepage paths.After triangulation on these polygons,a finite element mesh for 3D fracture ...The space block search technology is used to determine a connected three-dimensional fracture network in polygonal shapes,i.e.,seepage paths.After triangulation on these polygons,a finite element mesh for 3D fracture network seepage is obtained.Through introduction of the generalized Darcy's law,conservative equations for both fracture surface and fracture interactions are established.Combined with the boundary condition of Signorini's type,a partial differential equation(PDE) formulation is presented for the whole domain concerned.To solve this problem efficiently,an equivalent variational inequality(VI) formulation is given.With the penalized Heaviside function,a finite element procedure for unconfined seepage problem in 3D fracture network is developed.Through an example in a homogeneous rectangular dam,validity of the algorithm is verified.The analysis of an unconfined seepage problem in a complex fracture network shows that the proposed algorithm is very applicable to complex three-dimensional problems,and is effective in describing some interesting phenomenon usually encountered in practice,such as "preferential flow".展开更多
Let M be a compact orientable irreducible 3-manifold, and F be an essential connected closed surface in M which cuts M into two manifolds M1 and M2. If Mi has a minimal Heegaard splitting Mi = Vi∪Hi Wi with d(H1) ...Let M be a compact orientable irreducible 3-manifold, and F be an essential connected closed surface in M which cuts M into two manifolds M1 and M2. If Mi has a minimal Heegaard splitting Mi = Vi∪Hi Wi with d(H1) + d(H2) ≥ 2(g(M0 + g(M2) - g(F)) + 1, then g(M) = g(M1) + g(M2) - g(F).展开更多
Let M be a compact orientable 3-manifold with 0M connected. If V Us W is a Heegaard splitting of M with distance at least 6, then the 0-stabilization of V Us W along OM is unstabilized. Hence M has at least two unstab...Let M be a compact orientable 3-manifold with 0M connected. If V Us W is a Heegaard splitting of M with distance at least 6, then the 0-stabilization of V Us W along OM is unstabilized. Hence M has at least two unstabilized Heegaard splittings with different genera. The basic tool is a result on disk complex given by Masur and Schleimer.展开更多
Darcy's law only applying to the flow domain is extended to the entire fracture network domain including the dry domain.The partial differential equation(PDE) formulation for unconfined seepage flow problems for d...Darcy's law only applying to the flow domain is extended to the entire fracture network domain including the dry domain.The partial differential equation(PDE) formulation for unconfined seepage flow problems for discrete fracture network is established,in which a boundary condition of Signorini's type is prescribed over the potential seepage surfaces.In order to reduce the difficulty in selecting trial functions,a new variational inequality formulation is presented and mathematically proved to be equivalent to the PDE formulation.The numerical procedure based on the VI formulation is proposed and the corresponding algorithm has been developed.Since a continuous penalized Heaviside function is introduced to replace a jump function in finite element analysis,oscillation of numerical integration for facture elements cut by the free surface is eliminated and stability of numerical solution is assured.The numerical results from two typical examples demonstrate,on the one hand the effectiveness and robustness of the proposed method,and on the other hand the capability of predicting main seepage pathways in fractured rocks and flow rates out of the drainage system,which is very important for performance assessments and design optimization of complex drainage system.展开更多
Let X be a C1 vector field on a compact boundaryless Riemannian manifold M(dim M≥2),and A a compact invariant set of X.Suppose that A has a hyperbolic splitting,i.e.,T∧M = Es Eu with Es uniformly contracting and E...Let X be a C1 vector field on a compact boundaryless Riemannian manifold M(dim M≥2),and A a compact invariant set of X.Suppose that A has a hyperbolic splitting,i.e.,T∧M = Es Eu with Es uniformly contracting and Eu uniformly expanding.We prove that if,in addition,A is chain transitive,then the hyperbolic splitting is continuous,i.e.,A is a hyperbolic set.In general,when A is not necessarily chain transitive,the chain recurrent part is a hyperbolic set.Furthermore,we show that if the whole manifold M admits a hyperbolic splitting,then X has no singularity,and the flow is Anosov.展开更多
OBJECTIVE: To examine German controlled clinical trials on the therapeutic effects of acupuncture vs sham acupuncture, and to find whether there are problems with the conclusion that sham acupuncture has no significan...OBJECTIVE: To examine German controlled clinical trials on the therapeutic effects of acupuncture vs sham acupuncture, and to find whether there are problems with the conclusion that sham acupuncture has no significant deviation from acupuncture. METHODS: We focused on literature from the last ten years (2002-2011) included in PubMed about controlled clinical trials on acupuncture vs sham acupuncture carried out in Germany. The methods applied in sham acupuncture are summarized, and the difference between the acupuncture and sham groups were analyzed. We measured effects based on the following criteria: acupuncture is effective and superior to sham, acupuncture is effective but similar to sham, both of them have uncertainty regarding treatment effect, or no significant effect. Finally, we reviewed the hypotheses of different scholars on sham acupuncture and analyzed their results.RESULTS: Four types of controlled clinical trials including sham acupuncture on non-Traditional Chinese Medicine acupoints, minimal acupuncture on non-acupoints, placebo needle and sham laser acupuncture had varying results in the 57 articles analyzed. Some showed that acupuncture had a better effect than sham, while some suggest acupuncture and sham had similar effects. In all studies using sham acupuncture on non-therapeutic points, sham electrodes, and sham electro-acupuncture, the therapeutic effect was better than sham. Of the trials, 37 demonstrated that acupuncture had a better effect than sham acupuncture. Only nine trials found no significant difference between acupuncture and sham. Two controlled trials for the same condition (neck pain) conducted by two different German research institutes used the same control method, but reached contradictory conclusions. CONCLUSION: We found problems in conclusions based on results of controlled clinical trials of sham acupuncture in Germany. Therefore, there is still not enough evidence to support the statements that "acupuncture and sham acupuncture have no difference in treatment effect" and "acupuncture is just a placebo effect." The control methods of sham acupuncture used in Germany may not be standardized and may not be suitable for acupuncture clinical trial research. We suggest that research on the methodology of sham acupuncture should be given priority in the design of acupuncture trials in the future.展开更多
基金Supported by "863" High Tech Project of China (2001AA241121-10) Natural Science Foundation of Yunnan Province (2005C0023Q)~~
文摘An in vitro shoot regeneration procedure was developed in pepper ( Capsicum annuum L. ) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates ( respectively 100.0% and 58.3% ) and average number of adventitious bud from each explant (respectively 18.8 and 13.2) were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be: MS + 3.0 mg/L 6-BA + 1.0 mg/L IAA + 5.0 mg/L AgNO3 + 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.
基金This study was supported by grants from the Special Funds for Major State Basic Research(“973”)Project(G1999055902)of ChinaNational Natural Science Foundation of China(30225010,30170358)Knowledge Innovation Program(KSCX2-SW-303,KSCX-IOZ-07)of Chinese Academy of Sciences.
文摘In this study we used U0126, a potent and specific inhibitor of MEK, to study the roles of MEK/ERK/p90rsk signaling pathway in the meiotic cell cycle of mouse oocytes. The phosphorylation of MAP kinase and p90rsk in the oocytes treated with 1.5 μMU0126 was the same as that in oocytes cultured in drug-free medium. With 1.5 μM U0126 treatment, the spindles appeared normal as they formed in oocytes, but failed to maintain its structure.Instead, the spindle lost one pole or elongated extraordinarily. After further culture, some oocytes extruded gigantic polar bodies (>30 μm) that later divided into two small ones. Some oocytes underwent symmetric division and produced two equal-size daughter cells in which normal spindles formed. In oocytes with different division patterns,MAP kinase was normally phosphorylated. When the concentration of U0126 was increased to 15 mM, the phosphorylation of both MAPK and p90rsk were inhibited, while symmetric division was decreased. When incubating in medium containing 15 μM U0126 for 14 h, oocytes were activated, but part of them failed to emit polar bodies. MII oocytes were also activated by 15 μM U0126, at the same time the dephosphorylation of MAP kinase and p90rsk was observed. Our results indicate that 1) MEK plays important but not indispensable roles in microtubule organization;2) MEK keeps normal meiotic spindle morphology, targets peripheral spindle positioning and regulates asymmetric division by activating some unknown substrates other than MAP kinase/p90rsk; and 3) activation of MEK/ERK/p90rsk cascade maintains MII arrest in mouse oocytes.
基金supported by Program for New Century Excellent Talents in Universities of China under Grant No.NCET-06-0554the National Natural Science Foundation of China under Grant Nos.60677001 and 10747146+3 种基金the Science-Technology Fund of Anhui Province for Outstanding Youth under Grant No.06042087the Key Fund of the Ministry of Education of China under Grant No.206063the Natural Science Foundation of Guangdong Province under Grant Nos.06300345 and 7007806the Talent Foundation of High Education of Anhui Province for Outstanding Youth under Grant No.2009SQRZ056
文摘In this paper we propose a tripartite scheme for splitting an arbitrary 2-qubit quantum information by using two asymmetric W states as the quantum channel. In the schemem if the two recipients collaborate together, they can deterministically recover the quantum information by performing first a 4-qubit collective unitary operation and then two single-qubit unitary operations. In addition, since the asymmetric W states are employed as the quantum channel, the scheme is robust against decoherence.
基金Supported by Major State Basic Research Development Program of China(2012CB720305)the National Natural Science Foundation of China(21376162)
文摘The asymmetric breakups of a droplet in an axisymmetric cross-like microfluidic device are investigated by using a three-dimensional volume of fluid(VOF) multiphase numerical model. Two kinds of asymmetries(droplet location deviation from the symmetric geometry center and different flow rates at two symmetric outlets) generate asymmetric flow fields near the droplet, which results in the asymmetric breakup of the latter. Four typical breakup regimes(no breakup, one-side breakup, retraction breakup and direct breakup) have been observed.Two regime maps are plotted to describe the transition from one regime to another for the two types of different asymmetries, respectively. A power law model, which is based on the three critical factors(the capillary number,the asymmetry of flow fields and the initial volume ratio), is employed to predict the volume ratio of the two unequal daughter droplets generated in the direct breakup. The influences of capillary numbers and the asymmetries have been studied systematically in this paper. The larger the asymmetry is, the bigger the oneside breakup zone is. The larger the capillary number is, the more possible the breakup is in the direct breakup zone. When the radius of the initial droplet is 20 μm, the critical capillary numbers are 0.122, 0.128, 0.145,0.165, 0.192 and 0.226 for flow asymmetry factor AS= 0.05, 0.1, 0.2, 0.3, 0.4 and 0.5, respectively, in the flow system whose asymmetry is generated by location deviations. In the flow system whose asymmetry is generated by two different flow rates at two outlets, the critical capillary numbers are 0.121, 0.133, 0.145, 0.156 and 0.167 for AS= 1/21, 3/23, 1/5, 7/27 and 9/29, respectively.
基金supported by the National Natural Science Foundation of China(Grant No.51079110)the National Basic Research Program of China("973"Project)(Grant No.2011CB013506)
文摘The space block search technology is used to determine a connected three-dimensional fracture network in polygonal shapes,i.e.,seepage paths.After triangulation on these polygons,a finite element mesh for 3D fracture network seepage is obtained.Through introduction of the generalized Darcy's law,conservative equations for both fracture surface and fracture interactions are established.Combined with the boundary condition of Signorini's type,a partial differential equation(PDE) formulation is presented for the whole domain concerned.To solve this problem efficiently,an equivalent variational inequality(VI) formulation is given.With the penalized Heaviside function,a finite element procedure for unconfined seepage problem in 3D fracture network is developed.Through an example in a homogeneous rectangular dam,validity of the algorithm is verified.The analysis of an unconfined seepage problem in a complex fracture network shows that the proposed algorithm is very applicable to complex three-dimensional problems,and is effective in describing some interesting phenomenon usually encountered in practice,such as "preferential flow".
基金Project supported by the National Natural Science Foundation of China(No.10625102)
文摘Let M be a compact orientable irreducible 3-manifold, and F be an essential connected closed surface in M which cuts M into two manifolds M1 and M2. If Mi has a minimal Heegaard splitting Mi = Vi∪Hi Wi with d(H1) + d(H2) ≥ 2(g(M0 + g(M2) - g(F)) + 1, then g(M) = g(M1) + g(M2) - g(F).
基金supported by the National Natural Science Foundation of China(Nos.11271058,11171108)
文摘Let M be a compact orientable 3-manifold with 0M connected. If V Us W is a Heegaard splitting of M with distance at least 6, then the 0-stabilization of V Us W along OM is unstabilized. Hence M has at least two unstabilized Heegaard splittings with different genera. The basic tool is a result on disk complex given by Masur and Schleimer.
基金supported by the National Natural Science Foundation of China (Grant No. 51079110)the National Basic Research Program of China ("973" Project) (Grant No. 2011CB013506)
文摘Darcy's law only applying to the flow domain is extended to the entire fracture network domain including the dry domain.The partial differential equation(PDE) formulation for unconfined seepage flow problems for discrete fracture network is established,in which a boundary condition of Signorini's type is prescribed over the potential seepage surfaces.In order to reduce the difficulty in selecting trial functions,a new variational inequality formulation is presented and mathematically proved to be equivalent to the PDE formulation.The numerical procedure based on the VI formulation is proposed and the corresponding algorithm has been developed.Since a continuous penalized Heaviside function is introduced to replace a jump function in finite element analysis,oscillation of numerical integration for facture elements cut by the free surface is eliminated and stability of numerical solution is assured.The numerical results from two typical examples demonstrate,on the one hand the effectiveness and robustness of the proposed method,and on the other hand the capability of predicting main seepage pathways in fractured rocks and flow rates out of the drainage system,which is very important for performance assessments and design optimization of complex drainage system.
基金supported by the State Key Development Program for Basic Research of China(973 Project)(Grant No.2011CB808002)National Natural Science Foundation of China(Grant Nos.11025101 and 11231001)
文摘Let X be a C1 vector field on a compact boundaryless Riemannian manifold M(dim M≥2),and A a compact invariant set of X.Suppose that A has a hyperbolic splitting,i.e.,T∧M = Es Eu with Es uniformly contracting and Eu uniformly expanding.We prove that if,in addition,A is chain transitive,then the hyperbolic splitting is continuous,i.e.,A is a hyperbolic set.In general,when A is not necessarily chain transitive,the chain recurrent part is a hyperbolic set.Furthermore,we show that if the whole manifold M admits a hyperbolic splitting,then X has no singularity,and the flow is Anosov.
基金Supported by the Program of China Academy of Chinese Medical Sciences for Young Researchers in Medical Science(No.ZZ040305)
文摘OBJECTIVE: To examine German controlled clinical trials on the therapeutic effects of acupuncture vs sham acupuncture, and to find whether there are problems with the conclusion that sham acupuncture has no significant deviation from acupuncture. METHODS: We focused on literature from the last ten years (2002-2011) included in PubMed about controlled clinical trials on acupuncture vs sham acupuncture carried out in Germany. The methods applied in sham acupuncture are summarized, and the difference between the acupuncture and sham groups were analyzed. We measured effects based on the following criteria: acupuncture is effective and superior to sham, acupuncture is effective but similar to sham, both of them have uncertainty regarding treatment effect, or no significant effect. Finally, we reviewed the hypotheses of different scholars on sham acupuncture and analyzed their results.RESULTS: Four types of controlled clinical trials including sham acupuncture on non-Traditional Chinese Medicine acupoints, minimal acupuncture on non-acupoints, placebo needle and sham laser acupuncture had varying results in the 57 articles analyzed. Some showed that acupuncture had a better effect than sham, while some suggest acupuncture and sham had similar effects. In all studies using sham acupuncture on non-therapeutic points, sham electrodes, and sham electro-acupuncture, the therapeutic effect was better than sham. Of the trials, 37 demonstrated that acupuncture had a better effect than sham acupuncture. Only nine trials found no significant difference between acupuncture and sham. Two controlled trials for the same condition (neck pain) conducted by two different German research institutes used the same control method, but reached contradictory conclusions. CONCLUSION: We found problems in conclusions based on results of controlled clinical trials of sham acupuncture in Germany. Therefore, there is still not enough evidence to support the statements that "acupuncture and sham acupuncture have no difference in treatment effect" and "acupuncture is just a placebo effect." The control methods of sham acupuncture used in Germany may not be standardized and may not be suitable for acupuncture clinical trial research. We suggest that research on the methodology of sham acupuncture should be given priority in the design of acupuncture trials in the future.