目的:对太子参不同极性部位中的化学成分进行鉴定,并比较不同极性部位之间化学成分的差异。方法:将太子参饮片用80%乙醇制备总提取物后,分别用乙酸乙酯和正丁醇依次萃取,得到不同极性部位提取物。采用InfiniteC18色谱柱(4.6 mm × 2...目的:对太子参不同极性部位中的化学成分进行鉴定,并比较不同极性部位之间化学成分的差异。方法:将太子参饮片用80%乙醇制备总提取物后,分别用乙酸乙酯和正丁醇依次萃取,得到不同极性部位提取物。采用InfiniteC18色谱柱(4.6 mm × 250 mm, 5 μm),以乙腈(A)和0.2%甲酸溶液(B)为流动性进行梯度洗脱,流速为0.3 mL•min−1;离子源采用电喷雾离子源(ESI),扫描模型是正离子模式,结合Metlin数据库和文献报道的质谱信息对太子参不同极性部位中的化学成分进行鉴定。结果:从太子参乙酸乙酯和正丁醇部位中分别鉴定出10个和7个化合物。结论:本研究为太子参的药效物质基础研究提供了理论参考。展开更多
With Polygonatum cirrhifolium root as the donator, the wheat, cucumber and carrot as receiver plant, the potential allelopathic effects of petroleum extracts, acetic ether extracts, n-butanol extracts and aqueous extr...With Polygonatum cirrhifolium root as the donator, the wheat, cucumber and carrot as receiver plant, the potential allelopathic effects of petroleum extracts, acetic ether extracts, n-butanol extracts and aqueous extracts from donator on the seed germination and seedling growth of three receiver plants were studied by culture plate and filter-paper. The results showed that every extracts had different allelopathic effection on different receiver plant or on different position of same receiver plant. The allelopathy intensity were related to the concentration and strengthen along with the concentration raise. The different receiver plant had distinct sensitive to the the allelopathic effect, cucumber was more sensitive than carrot and wheat. Every extracts had inhibition against seed germination and seedling growth, but inhibited root growth at high concentration and stimulated it at low concentration. In a word, every extracts had more impact on the seedling growth, however, had weak inhibition against seed germination. The average allelopathic effection of acetic ether extracts was more stronger, then n-butanol extracts, aqueous extracts and petroleum extracts. The acetic ether extracts had extremely strong inhibition on the growth of seedling and root, and n-butanol extracts on the seed germination also, which implied the Polygonatum cirrhifolium contained the allelochemicals with high polarity. In rhizosphere soil of P. cirrhifolium , the root and seedlings growth of wheat was stimulated, while cucumber and carrot was inhibited.展开更多
文摘目的:对太子参不同极性部位中的化学成分进行鉴定,并比较不同极性部位之间化学成分的差异。方法:将太子参饮片用80%乙醇制备总提取物后,分别用乙酸乙酯和正丁醇依次萃取,得到不同极性部位提取物。采用InfiniteC18色谱柱(4.6 mm × 250 mm, 5 μm),以乙腈(A)和0.2%甲酸溶液(B)为流动性进行梯度洗脱,流速为0.3 mL•min−1;离子源采用电喷雾离子源(ESI),扫描模型是正离子模式,结合Metlin数据库和文献报道的质谱信息对太子参不同极性部位中的化学成分进行鉴定。结果:从太子参乙酸乙酯和正丁醇部位中分别鉴定出10个和7个化合物。结论:本研究为太子参的药效物质基础研究提供了理论参考。
文摘With Polygonatum cirrhifolium root as the donator, the wheat, cucumber and carrot as receiver plant, the potential allelopathic effects of petroleum extracts, acetic ether extracts, n-butanol extracts and aqueous extracts from donator on the seed germination and seedling growth of three receiver plants were studied by culture plate and filter-paper. The results showed that every extracts had different allelopathic effection on different receiver plant or on different position of same receiver plant. The allelopathy intensity were related to the concentration and strengthen along with the concentration raise. The different receiver plant had distinct sensitive to the the allelopathic effect, cucumber was more sensitive than carrot and wheat. Every extracts had inhibition against seed germination and seedling growth, but inhibited root growth at high concentration and stimulated it at low concentration. In a word, every extracts had more impact on the seedling growth, however, had weak inhibition against seed germination. The average allelopathic effection of acetic ether extracts was more stronger, then n-butanol extracts, aqueous extracts and petroleum extracts. The acetic ether extracts had extremely strong inhibition on the growth of seedling and root, and n-butanol extracts on the seed germination also, which implied the Polygonatum cirrhifolium contained the allelochemicals with high polarity. In rhizosphere soil of P. cirrhifolium , the root and seedlings growth of wheat was stimulated, while cucumber and carrot was inhibited.
