血管生成素样蛋白4(Angiopoietin-Like Protein 4,ANGPTL4)是一种分泌型蛋白,作为脂蛋白脂酶(Lipoprteinlipase,LPL)的内源性抑制剂参与脂肪代谢、糖代谢等生理活动的调节,尤其对脂肪代谢起着重要的调控作用。开展ANGPTL4蛋白因子的生...血管生成素样蛋白4(Angiopoietin-Like Protein 4,ANGPTL4)是一种分泌型蛋白,作为脂蛋白脂酶(Lipoprteinlipase,LPL)的内源性抑制剂参与脂肪代谢、糖代谢等生理活动的调节,尤其对脂肪代谢起着重要的调控作用。开展ANGPTL4蛋白因子的生物学信息分析对研究其调控机制具有重要意义。本文采用生物信息学方法对人、鼠、畜禽和鱼的ANGPTL4基因序列、理化性质等进行了分析,并着重对猪、鸡、鸭、粉脚雁、黄颡鱼5种动物的ANGPTL4氨基酸、分子特征、蛋白结构进行分析。结果显示反刍动物牛羊、禽类鸭和粉脚雁ANGPTL4的编码序列(CDS)相似度最高;氨基酸组成中都含有20种常见的氨基酸,亮氨酸(Leu)含量最高,半胱氨酸(Cys)含量最低;ANGPTL4不存在跨膜结构并含有信号肽;在对ANGPTL4磷酸化位点的预测中发现含有激酶和LPL结合位点群,揭示ANGPTL4蛋白参与机体脂质代谢过程。展开更多
According to different mechanisms of dormancy,seeds of Gagea nigra L.Z.Shue were treated with different methods so as to investigate the effects of different treatments on seed germination of G.nigra.The results showe...According to different mechanisms of dormancy,seeds of Gagea nigra L.Z.Shue were treated with different methods so as to investigate the effects of different treatments on seed germination of G.nigra.The results showed that at 4 ℃,the seeds of G.nigra began to germinate on day 32 with germination period of 80 d and germination rate of 72%;room temperature（25 ℃） and acid and alkali treatments were all unsuitable for the germination of G.nigra seeds;no significant differences were found in germination rate of G.nigra seeds among warm water（50℃）,ultrasound and 200 mg/L GA_3treatments（P 0.05）;seed coat-removing treatment effectively promoted the germination of G.nigra seeds（P =0.002 0.01） with germination rate of 88%;seed coat piercing ＋ 200 mg/L GA_3 and 500 mg/L GA_3＋15% H_2O_2 treatments effectively promoted the germination of G.nigra seeds with germination rates of 84% and 82%,respectively（P0.05）;the germination vigor of G.nigra seeds in the seed coat piercing ＋ 200 mg/L GA_3 treatment was highest,and it was significantly higher than those in the other treatments.展开更多
文摘血管生成素样蛋白4(Angiopoietin-Like Protein 4,ANGPTL4)是一种分泌型蛋白,作为脂蛋白脂酶(Lipoprteinlipase,LPL)的内源性抑制剂参与脂肪代谢、糖代谢等生理活动的调节,尤其对脂肪代谢起着重要的调控作用。开展ANGPTL4蛋白因子的生物学信息分析对研究其调控机制具有重要意义。本文采用生物信息学方法对人、鼠、畜禽和鱼的ANGPTL4基因序列、理化性质等进行了分析,并着重对猪、鸡、鸭、粉脚雁、黄颡鱼5种动物的ANGPTL4氨基酸、分子特征、蛋白结构进行分析。结果显示反刍动物牛羊、禽类鸭和粉脚雁ANGPTL4的编码序列(CDS)相似度最高;氨基酸组成中都含有20种常见的氨基酸,亮氨酸(Leu)含量最高,半胱氨酸(Cys)含量最低;ANGPTL4不存在跨膜结构并含有信号肽;在对ANGPTL4磷酸化位点的预测中发现含有激酶和LPL结合位点群,揭示ANGPTL4蛋白参与机体脂质代谢过程。
文摘According to different mechanisms of dormancy,seeds of Gagea nigra L.Z.Shue were treated with different methods so as to investigate the effects of different treatments on seed germination of G.nigra.The results showed that at 4 ℃,the seeds of G.nigra began to germinate on day 32 with germination period of 80 d and germination rate of 72%;room temperature（25 ℃） and acid and alkali treatments were all unsuitable for the germination of G.nigra seeds;no significant differences were found in germination rate of G.nigra seeds among warm water（50℃）,ultrasound and 200 mg/L GA_3treatments（P 0.05）;seed coat-removing treatment effectively promoted the germination of G.nigra seeds（P =0.002 0.01） with germination rate of 88%;seed coat piercing ＋ 200 mg/L GA_3 and 500 mg/L GA_3＋15% H_2O_2 treatments effectively promoted the germination of G.nigra seeds with germination rates of 84% and 82%,respectively（P0.05）;the germination vigor of G.nigra seeds in the seed coat piercing ＋ 200 mg/L GA_3 treatment was highest,and it was significantly higher than those in the other treatments.
