[Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua （Hbner）. [Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK1...[Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua （Hbner）. [Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK12 and SGK321 were used to feed S. exigua neonates respectively. Survival larvae and dead ones were counted on the 3rd,the 7th,the 10th,the 16th and the 19th day; meanwhile,the pupae amount was recorded,and the pupae weight was measured at the 24th h after pupation. [Result] The survival curves,pupation rates and pupae weights of S. exigua feeding on different tissues of transgenic cotton were not significantly different from those of S. exigua feeding on the corresponding tissues of conventional cotton; pupation rate of S. exigua feeding on different leaves of the same cotton variety were not significantly different from each other,but all higher than that of S. exigua feeding on the flowers of that cotton; and there were no differences among pupation weights of S. exigua feeding on different leaves or flowers of the same cotton variety. [Conclusion] Transgenic cotton showed weak resistance to S. exigua. Hence,in the transgenic cotton fields,more attention should be paid to occurrence trend of S. exigua and its control.展开更多
[Objective] This study was conducted to determinate the contents of poliumoside and forsythoside B in Callicarpa kwangtungensis,so as to reveal the distribution of two index components（poliumoside and forsythoside B...[Objective] This study was conducted to determinate the contents of poliumoside and forsythoside B in Callicarpa kwangtungensis,so as to reveal the distribution of two index components（poliumoside and forsythoside B） in the plant and to compare the contents of these components in the medicinal materials from different habitats.[Methods] Comparison and analysis of the contents of the two target components in different parts（root,branch,leaf and fruit） of Callicarpa kwangtungensis from the same habitat and in Callicarpa kwangtungensis from different production areas were performed according to the pharmacopoeia standard.[Result]The highest content of poliumoside wasdetected in fruit,and the content of forsythoside B was the highest in the branch.The contents of the two kinds of components were in order of branch fruit leafroot;The contents of the components were four times higher than China pharmacopeia（0.5%） in Jian,Tonggu,Anyuan,and Ganzhou of Jiangxi Province and the fifth area,Taijiang County of Qiandongnan Miao and Dong Autonomous Prefecture（QMDAP）,among which Ganzhou and Taijiang showed the highest contents.[Conclusion] The differences of poliumoside and forsythoside B were obvious in different habitats and different parts of Callicarpa kwangtungensis.展开更多
Orthogonal design was used to observe the gastrointestinal peristalsis in normal andatropine-treated mice after electroacupuncture was applied,singly or in combination,atNeiguan(P 6),Pishu(UB 20)and Zusanli(St 36).The...Orthogonal design was used to observe the gastrointestinal peristalsis in normal andatropine-treated mice after electroacupuncture was applied,singly or in combination,atNeiguan(P 6),Pishu(UB 20)and Zusanli(St 36).The results showed that:1)electroacupuncture has no significant effect on the gastrointestinal peristalsis in normalmice;2)Pishu(UB 20)was significantly antagonistic to Zusanli(St 36)in normal mice;3)the decreased gastrointestinal peristalsis in atropine-treated mice was markedly promotedby electroacupuncture at Zusanli(St 36);and 4)Neiguan(P 6)was significantlyantagonistic to Pishu(UB 20)in atropine-treated mice.The results indicated that thereciprocal actions among acupoints should be taken into consideration for pointprescription.展开更多
The dose distribution in the lung is inhomogeneous. The dose to the hasal cell layer of trachea and main bronchi is much higher than the dose to total lung both for rabbits at different ages and for different animals....The dose distribution in the lung is inhomogeneous. The dose to the hasal cell layer of trachea and main bronchi is much higher than the dose to total lung both for rabbits at different ages and for different animals. A maximum value of the dose to lung tissue for rabbits at ages of 20-40d is observed. The dose decreases with increasing body weight. The relationship between the dose and body weight can be described by a power function. The dose to total lung increases exponentially with the minute breathing volume per unit of lung weight.展开更多
As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried root...As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried roots of Bupleurum chinense DC.and Bupleurum scorzonerifolium Willd.Among of diverse chemical components,saikosaponins are the key active components of the herb medicine.In the present study,we established a method of high performance liquid chromatography（HPLC） coupled with evaporative light scattering detection（ELSD） for simultaneous determination of saikosaponin a,c and d in root,stem,leaf and flower of Bupleurum chinense（B chinense） collected from different areas of Shanxi Province,China.The results from 16 samples of root,stem,leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan,Xishan,Tianlongshan and Pangquangou were 4.26 mg/g,3.22 mg/g,4.23 mg/g and 3.05 mg/g,respectively.However,there was scarcely any saikosaponins in the stem,leaf and flower of B chinense collected from above-mentioned areas.The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri.The result also confirmed that the root of B chinense was the best medicinal part.展开更多
基金Supported by National Transgenic Major Project ( Safe Monitoring Technique of Transgenic Organism 2008ZX08012-004)~~
文摘[Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua （Hbner）. [Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK12 and SGK321 were used to feed S. exigua neonates respectively. Survival larvae and dead ones were counted on the 3rd,the 7th,the 10th,the 16th and the 19th day; meanwhile,the pupae amount was recorded,and the pupae weight was measured at the 24th h after pupation. [Result] The survival curves,pupation rates and pupae weights of S. exigua feeding on different tissues of transgenic cotton were not significantly different from those of S. exigua feeding on the corresponding tissues of conventional cotton; pupation rate of S. exigua feeding on different leaves of the same cotton variety were not significantly different from each other,but all higher than that of S. exigua feeding on the flowers of that cotton; and there were no differences among pupation weights of S. exigua feeding on different leaves or flowers of the same cotton variety. [Conclusion] Transgenic cotton showed weak resistance to S. exigua. Hence,in the transgenic cotton fields,more attention should be paid to occurrence trend of S. exigua and its control.
文摘[Objective] This study was conducted to determinate the contents of poliumoside and forsythoside B in Callicarpa kwangtungensis,so as to reveal the distribution of two index components（poliumoside and forsythoside B） in the plant and to compare the contents of these components in the medicinal materials from different habitats.[Methods] Comparison and analysis of the contents of the two target components in different parts（root,branch,leaf and fruit） of Callicarpa kwangtungensis from the same habitat and in Callicarpa kwangtungensis from different production areas were performed according to the pharmacopoeia standard.[Result]The highest content of poliumoside wasdetected in fruit,and the content of forsythoside B was the highest in the branch.The contents of the two kinds of components were in order of branch fruit leafroot;The contents of the components were four times higher than China pharmacopeia（0.5%） in Jian,Tonggu,Anyuan,and Ganzhou of Jiangxi Province and the fifth area,Taijiang County of Qiandongnan Miao and Dong Autonomous Prefecture（QMDAP）,among which Ganzhou and Taijiang showed the highest contents.[Conclusion] The differences of poliumoside and forsythoside B were obvious in different habitats and different parts of Callicarpa kwangtungensis.
文摘Orthogonal design was used to observe the gastrointestinal peristalsis in normal andatropine-treated mice after electroacupuncture was applied,singly or in combination,atNeiguan(P 6),Pishu(UB 20)and Zusanli(St 36).The results showed that:1)electroacupuncture has no significant effect on the gastrointestinal peristalsis in normalmice;2)Pishu(UB 20)was significantly antagonistic to Zusanli(St 36)in normal mice;3)the decreased gastrointestinal peristalsis in atropine-treated mice was markedly promotedby electroacupuncture at Zusanli(St 36);and 4)Neiguan(P 6)was significantlyantagonistic to Pishu(UB 20)in atropine-treated mice.The results indicated that thereciprocal actions among acupoints should be taken into consideration for pointprescription.
文摘The dose distribution in the lung is inhomogeneous. The dose to the hasal cell layer of trachea and main bronchi is much higher than the dose to total lung both for rabbits at different ages and for different animals. A maximum value of the dose to lung tissue for rabbits at ages of 20-40d is observed. The dose decreases with increasing body weight. The relationship between the dose and body weight can be described by a power function. The dose to total lung increases exponentially with the minute breathing volume per unit of lung weight.
基金Shanxi Educational Committee(Grant No.20111113)Shanxi Science and Technology Department(Grant No.2016ZD0201)
文摘As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri（Chai-Hu） is commonly used for the treatment of influenza,which is derived from the dried roots of Bupleurum chinense DC.and Bupleurum scorzonerifolium Willd.Among of diverse chemical components,saikosaponins are the key active components of the herb medicine.In the present study,we established a method of high performance liquid chromatography（HPLC） coupled with evaporative light scattering detection（ELSD） for simultaneous determination of saikosaponin a,c and d in root,stem,leaf and flower of Bupleurum chinense（B chinense） collected from different areas of Shanxi Province,China.The results from 16 samples of root,stem,leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan,Xishan,Tianlongshan and Pangquangou were 4.26 mg/g,3.22 mg/g,4.23 mg/g and 3.05 mg/g,respectively.However,there was scarcely any saikosaponins in the stem,leaf and flower of B chinense collected from above-mentioned areas.The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri.The result also confirmed that the root of B chinense was the best medicinal part.
