AIM： To identify molecular markers shared across South African esophageal squamous cell carcinoma （ESCC） cell lines using o/togenetics, fluorescence in situ hybridization （FISH） and single nucleotide polymorphism...AIM： To identify molecular markers shared across South African esophageal squamous cell carcinoma （ESCC） cell lines using o/togenetics, fluorescence in situ hybridization （FISH） and single nucleotide polymorphism （SNP） array copy number analysis. METHODS： We used conventional cytogenetics, FISH, and multicolor FISH to characterize the chromosomal rearrangements of five ESCC cell lines established in South Africa. The whole genome copy number profile was established from 250K SNP arrays, and data was analyzed with the CNAT 4.0 and GISTIC software. tions involved the following chromosomal regions and genes： 11q13.3 （CCND1, FGF3, FGF4, FGF19, MYEOV）, 8q24.21（C-MYC, FAM84B）, 11q22.1-q22.3 （B[RC2, BIRC3）, 5p15.2 （CTNND2）, 3qll.2-q12.2 （MINA） and 18p11.32 （TYMS, YES1）. The significant deletions included 1p31.2-p31.1 （CTH, GADD45a, DIRAS3）, 2q22.1 （LRPIB）, 3p12.1-p14.2 （FHIT）, 4q22.1-q32.1 （CASP6, SMAD1）, 8p23.2-q11.1 （BNIP3L） and 18q21.1-q21.2 （SMAD4, DCC）. The 3p11.2 translocation breakpoint was shared across four cell lines, supporting a role for genes involved at this site, in particular, the EPHA3 gene which has previously been reported to be deleted in ESCC.CONCLUSION： The finding that a significant number of genes that were amplified （FGF3, FGF4, FGF19, CCND1 and C-MYC） or deleted （SFRP2 gene） are involved in the Wnt and fibroblast growth factor signaling pathways, suggests that these pathways may be activated in these cell lines.展开更多
The territorial consideration is enclosed in the development of network economy and e-Business, and there is no departure of the cultivation of e-Business from the establishment of territorial competitiveness. The tra...The territorial consideration is enclosed in the development of network economy and e-Business, and there is no departure of the cultivation of e-Business from the establishment of territorial competitiveness. The traditional theory of the territorial competitiveness has come across a serious impact from the Internet. So the development of Intemet infrastructures and institutional environment would be something new to the theory of territorial competitiveness in the Internet era.展开更多
Traditional fracture analysis is based on fracture mechanics and damage mechanics. They focus on the propagation of the fracture. However, their propagation criterions are not easily applied in practice and the curren...Traditional fracture analysis is based on fracture mechanics and damage mechanics. They focus on the propagation of the fracture. However, their propagation criterions are not easily applied in practice and the current analysis is limited in planar problem. This paper presents a new theory that the occurrence of the unbalanced force (derived from the Deformation Reinforcement Theory) could be the criterion of the initiation of the fracture, and the distribution area and propagation of the unbalanced force could be the indication of the fracture propagation direction. By aggregate analysis with Stress Intensity Factor (SIF) criterion, the unbalanced force actually is the opposite external load that is the SIF difference incurred between the external loads and permitted by the structure. Numerical simulation and physical experiments on pre-fracture cuboid rock specimens proved that the occurrence of the unbalanced force could be the initiation of the fracture. Mesh size dependence was also considered by analysis of different mesh size finite element gravity dam models. Furthermore, the theory was applied to the feasibility analysis of the Baihetan arch dam together with physical experiments in order to evaluate the fracture propagation of dam heel. The results show that it is an effective way to use unbalanced force to analyze the fracture initiation and propagation when performing 3-dimensional nonlinear FEM calculation.展开更多
文摘AIM： To identify molecular markers shared across South African esophageal squamous cell carcinoma （ESCC） cell lines using o/togenetics, fluorescence in situ hybridization （FISH） and single nucleotide polymorphism （SNP） array copy number analysis. METHODS： We used conventional cytogenetics, FISH, and multicolor FISH to characterize the chromosomal rearrangements of five ESCC cell lines established in South Africa. The whole genome copy number profile was established from 250K SNP arrays, and data was analyzed with the CNAT 4.0 and GISTIC software. tions involved the following chromosomal regions and genes： 11q13.3 （CCND1, FGF3, FGF4, FGF19, MYEOV）, 8q24.21（C-MYC, FAM84B）, 11q22.1-q22.3 （B[RC2, BIRC3）, 5p15.2 （CTNND2）, 3qll.2-q12.2 （MINA） and 18p11.32 （TYMS, YES1）. The significant deletions included 1p31.2-p31.1 （CTH, GADD45a, DIRAS3）, 2q22.1 （LRPIB）, 3p12.1-p14.2 （FHIT）, 4q22.1-q32.1 （CASP6, SMAD1）, 8p23.2-q11.1 （BNIP3L） and 18q21.1-q21.2 （SMAD4, DCC）. The 3p11.2 translocation breakpoint was shared across four cell lines, supporting a role for genes involved at this site, in particular, the EPHA3 gene which has previously been reported to be deleted in ESCC.CONCLUSION： The finding that a significant number of genes that were amplified （FGF3, FGF4, FGF19, CCND1 and C-MYC） or deleted （SFRP2 gene） are involved in the Wnt and fibroblast growth factor signaling pathways, suggests that these pathways may be activated in these cell lines.
文摘The territorial consideration is enclosed in the development of network economy and e-Business, and there is no departure of the cultivation of e-Business from the establishment of territorial competitiveness. The traditional theory of the territorial competitiveness has come across a serious impact from the Internet. So the development of Intemet infrastructures and institutional environment would be something new to the theory of territorial competitiveness in the Internet era.
基金supported by the National Natural Science Foundation of China (Grant No. 50709014)China National Funds for Distinguished Young Scientists (Grant No. 50925931)State Key Laboratory of Hydroscience and Engineering of China (Grant No. 2008-TC-2)
文摘Traditional fracture analysis is based on fracture mechanics and damage mechanics. They focus on the propagation of the fracture. However, their propagation criterions are not easily applied in practice and the current analysis is limited in planar problem. This paper presents a new theory that the occurrence of the unbalanced force (derived from the Deformation Reinforcement Theory) could be the criterion of the initiation of the fracture, and the distribution area and propagation of the unbalanced force could be the indication of the fracture propagation direction. By aggregate analysis with Stress Intensity Factor (SIF) criterion, the unbalanced force actually is the opposite external load that is the SIF difference incurred between the external loads and permitted by the structure. Numerical simulation and physical experiments on pre-fracture cuboid rock specimens proved that the occurrence of the unbalanced force could be the initiation of the fracture. Mesh size dependence was also considered by analysis of different mesh size finite element gravity dam models. Furthermore, the theory was applied to the feasibility analysis of the Baihetan arch dam together with physical experiments in order to evaluate the fracture propagation of dam heel. The results show that it is an effective way to use unbalanced force to analyze the fracture initiation and propagation when performing 3-dimensional nonlinear FEM calculation.
