[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional tou...[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio.[Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR;no obvious electrophoretic band but dispersive product was obtained by normal touchdown PCR;ideal result was obtained by the abnormal touchdown PCR that three evident electrophoretic bands and much more Sox genes were amplified.[Conclusion] The research provided theoretical basis for the optimization and selection of PCR amplification conditions of the large-family genes.展开更多
Through the analysis and comparison of shortcomings and advantages of existing technologies on object modeling in 3D applications,we propose a new modeling method for virtual scene based on multi-view image sequence t...Through the analysis and comparison of shortcomings and advantages of existing technologies on object modeling in 3D applications,we propose a new modeling method for virtual scene based on multi-view image sequence to model irregular objects efficiently in 3D application.In 3D scene,this method can get better visual effect by tracking the viewer's real-time perspective position and projecting the photos from different perspectives dynamically.The philosophy of design,the steps of development and some other relevant topics are discussed in details,and the validity of the algorithm is analyzed.The results demonstrate that this method represents more superiority on simulating irregular objects by applying it to the modeling of virtual museum.展开更多
Internal waves can bring nutrients to the upper level of water bodies and facilitate phytoplankton photosynthesis.Internal waves occur frequently in the northern portion of the South China Sea and inflict an important...Internal waves can bring nutrients to the upper level of water bodies and facilitate phytoplankton photosynthesis.Internal waves occur frequently in the northern portion of the South China Sea and inflict an important effect on chlorophyll a distribution.In this study,in-situ observation and satellite remote sensing data were used to study the effects of internal waves on chlorophyll a distribution.Based on the in-situ observations,lower chlorophyll a concentrations were present in the middle and bottom level in areas in which internal waves occur frequently,while the surface chlorophyll a distribution increased irregularly,and a small area with relatively higher chlorophyll a concentrations was observed in the area around the Dongsha Island.Satellite remote sensing showed that the chlorophyll a concentration increased in the area near Dongsha Island,where internal waves frequently occurred.The results of the increased chlorophyll a concentration in the surface water near Dongsha Island in the northern portion of the South China Sea indicated that internal waves could uplift phytoplankton and facilitate phytoplankton growth.展开更多
In this paper the anomalous magnetic dipole moment ofmuon in the littlest Higgs （LH） model is studied at one-loop level. We discuss the dependence of the contributions on the global symmetry breaking scale f, mizing...In this paper the anomalous magnetic dipole moment ofmuon in the littlest Higgs （LH） model is studied at one-loop level. We discuss the dependence of the contributions on the global symmetry breaking scale f, mizing angles c` and , and the Higgs triplet vacuum expectation value v＇ in the electroweak precision data preferring ranges. We find that the LH model can give a relatively small, but non-negligible extra weak contribution to the muon anomalous magnetic moment and can reduce the deviation of △aμ from 2.6σ for the SM to 2.5σ for the LH model.展开更多
In this study,we theoretically investigate the dynamic indentation for measuring the loss (damping) factor of a linear viscoelastic material from its indentation response.A rigid indenter with arbitrary tip profile is...In this study,we theoretically investigate the dynamic indentation for measuring the loss (damping) factor of a linear viscoelastic material from its indentation response.A rigid indenter with arbitrary tip profile is assumed to indent into a viscoelastic substrate with arbitrary shape.We perform a theoretical analysis and identify the conditions under which the loss factor of the material can be determined from the phase angle between the applied harmonic indentation load and the corresponding harmonic displacement,a directly measurable quantity in a dynamic indentation test.To validate the conclusion drawn from our theoretical analysis,a series of numerical experiments are performed,including the spherical indentation of a soft layer with irregular surface morphology bonded to a rigid substrate,a conical indenter with tip defects indenting into a half-spherical particle,and the indentation of porous materials.This study may facilitate the use of the dynamic indentation technique to evaluate the damping properties of linear viscoelastic materials,including some advanced polymers and biological soft tissues.展开更多
Microbial lumps have been found in the Maozhuang Stage(Cambrian Series 2) in the Liangcheng section,Shandong Province,China.Their macro-and micro-scale features distinguish them from other carbonate particles such as ...Microbial lumps have been found in the Maozhuang Stage(Cambrian Series 2) in the Liangcheng section,Shandong Province,China.Their macro-and micro-scale features distinguish them from other carbonate particles such as intraclasts and oncoids.Most lumps have a coarse sand to pebble size.They were mostly rounded during sedimentation or combined to form irregular shapes.A distinct boundary is present between the lumps and the surrounding cement of sparry calcite.The lumps contain either no core at all or several irregular "cores",without a distinct inner texture.They are composed mainly of calcified(micritized) microbes,most likely cyanobacteria.The microbes drilled holes in carbonate grains,after which lumps formed by micritization,cementation,and agglutination by an extracellular polymeric substance(EPS) in a high-energy shallow-marine,subtidal environment.As the lumps are directly related to microbes,they should be considered as microbialites.展开更多
文摘[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio.[Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR;no obvious electrophoretic band but dispersive product was obtained by normal touchdown PCR;ideal result was obtained by the abnormal touchdown PCR that three evident electrophoretic bands and much more Sox genes were amplified.[Conclusion] The research provided theoretical basis for the optimization and selection of PCR amplification conditions of the large-family genes.
文摘Through the analysis and comparison of shortcomings and advantages of existing technologies on object modeling in 3D applications,we propose a new modeling method for virtual scene based on multi-view image sequence to model irregular objects efficiently in 3D application.In 3D scene,this method can get better visual effect by tracking the viewer's real-time perspective position and projecting the photos from different perspectives dynamically.The philosophy of design,the steps of development and some other relevant topics are discussed in details,and the validity of the algorithm is analyzed.The results demonstrate that this method represents more superiority on simulating irregular objects by applying it to the modeling of virtual museum.
基金Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX1-YW-12-01)the National High Technology Research and Development Program of China (863 Program) (No.2008AA09Z112)+4 种基金the National Basic Research Program of China (973 Program) (No.2010CB951200)the National Natural Sciences Foundation of China (No.40876092)the Program of Guangdong Provincial Science & Technology (No.2008B030303026)the Natural Sciences Foundation of Guangdong Province (No.8351030101000002)the Project of Knowledge Innovation of the South China Sea Institute of Oceanology (No.LYQY200701)
文摘Internal waves can bring nutrients to the upper level of water bodies and facilitate phytoplankton photosynthesis.Internal waves occur frequently in the northern portion of the South China Sea and inflict an important effect on chlorophyll a distribution.In this study,in-situ observation and satellite remote sensing data were used to study the effects of internal waves on chlorophyll a distribution.Based on the in-situ observations,lower chlorophyll a concentrations were present in the middle and bottom level in areas in which internal waves occur frequently,while the surface chlorophyll a distribution increased irregularly,and a small area with relatively higher chlorophyll a concentrations was observed in the area around the Dongsha Island.Satellite remote sensing showed that the chlorophyll a concentration increased in the area near Dongsha Island,where internal waves frequently occurred.The results of the increased chlorophyll a concentration in the surface water near Dongsha Island in the northern portion of the South China Sea indicated that internal waves could uplift phytoplankton and facilitate phytoplankton growth.
基金The project supported in part by National Natural Science Foundation of China and Special Fund sponsored by the Chinese Academy of Sciences
文摘In this paper the anomalous magnetic dipole moment ofmuon in the littlest Higgs （LH） model is studied at one-loop level. We discuss the dependence of the contributions on the global symmetry breaking scale f, mizing angles c` and , and the Higgs triplet vacuum expectation value v＇ in the electroweak precision data preferring ranges. We find that the LH model can give a relatively small, but non-negligible extra weak contribution to the muon anomalous magnetic moment and can reduce the deviation of △aμ from 2.6σ for the SM to 2.5σ for the LH model.
基金supported by the National Natural Science Foundation of China(Grant Nos. 10972112,10525210,and 10732050)the National Basic Research Program of China(Grant Nos. 2010CB631005)
文摘In this study,we theoretically investigate the dynamic indentation for measuring the loss (damping) factor of a linear viscoelastic material from its indentation response.A rigid indenter with arbitrary tip profile is assumed to indent into a viscoelastic substrate with arbitrary shape.We perform a theoretical analysis and identify the conditions under which the loss factor of the material can be determined from the phase angle between the applied harmonic indentation load and the corresponding harmonic displacement,a directly measurable quantity in a dynamic indentation test.To validate the conclusion drawn from our theoretical analysis,a series of numerical experiments are performed,including the spherical indentation of a soft layer with irregular surface morphology bonded to a rigid substrate,a conical indenter with tip defects indenting into a half-spherical particle,and the indentation of porous materials.This study may facilitate the use of the dynamic indentation technique to evaluate the damping properties of linear viscoelastic materials,including some advanced polymers and biological soft tissues.
基金supported by National Natural Science Foundation of China(Grant No.40972043)Ministry of Education Key Project of Science and Technology(Grant No.211097)+2 种基金Shandong Province Young and Middle-Aged Scientists Research Awards Fund(Grants Nos.BS2009HZ020,2010BSE06022)Science Project of Universities in Shandong Province(Grants Nos.G08LD01,J09LE04)SDUST Research Fund(Grant No.2010KYTD103)
文摘Microbial lumps have been found in the Maozhuang Stage(Cambrian Series 2) in the Liangcheng section,Shandong Province,China.Their macro-and micro-scale features distinguish them from other carbonate particles such as intraclasts and oncoids.Most lumps have a coarse sand to pebble size.They were mostly rounded during sedimentation or combined to form irregular shapes.A distinct boundary is present between the lumps and the surrounding cement of sparry calcite.The lumps contain either no core at all or several irregular "cores",without a distinct inner texture.They are composed mainly of calcified(micritized) microbes,most likely cyanobacteria.The microbes drilled holes in carbonate grains,after which lumps formed by micritization,cementation,and agglutination by an extracellular polymeric substance(EPS) in a high-energy shallow-marine,subtidal environment.As the lumps are directly related to microbes,they should be considered as microbialites.
