Objective: To evaluate the regulatory effects of Banxia (Pinellia ternata, P) and Huanglian (Coptis chinensis, C) drugpair, derived from Banxiaxiexin soup, on the gastrointestinal movement of mice with functional...Objective: To evaluate the regulatory effects of Banxia (Pinellia ternata, P) and Huanglian (Coptis chinensis, C) drugpair, derived from Banxiaxiexin soup, on the gastrointestinal movement of mice with functional dyspepsia. Methods:Mice were treated with different proportions of the P and C drug pair (1:1, 3:1, and 4:1) for 10 days, and subsequentlyinjected with atropine (ATR) or neostigmine (NEO). The effects of the different proportions of P and C were evaluatedbased on the alvine advance rate. In addition, we used the same modeling method used in the first experiment andadministered P: C at ratio of 3:1 and at different doses respectively (4.68 g/L, 2.34 g/L, and 1.17 g/L), and tested levelsof the gastrointestinal hormones, gastrin (GAS), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) in thesmall intestinal tissue using an enzyme-linked immunosorbent assay. Results: In the groups of NEO-induced mice, P:Cat ratios of 1:1, 3:1, and 4:1 significantly reduced the alvine advance rate compared with the NEO model group (P =0.003, P = 0.012 and P = 0.021, respectively). In the groups of ATR-induced mice, only P:1 at ratio of 3:1 significantlyincreased the alvine advance rate compared with the ATR model group (P = 0.007). After exposure to P: C at ratio of 3:1and at different dose, the GAS level was lower in the low-, medium-, and high-dose NEO groups than that in the NEOmodel group (P = 0.001, P = 0.004, and P = 0.003, respectively). The VIP levels were higher in the medium-andhigh-dose NEO groups than that in the NEO model group (P = 0.004 and P = 0.002, respectively). In addition, the SSlevel increased in the NEO medium-dose group compared with that in the NEO model group (P = 0.002). The GAS levelwas higher in the ATR medium- and high-dose groups than in the ATR model group (P = 0.007 and P = 0.021,respectively). The VIP level was lower in the ATR low-, medium-, and high-dose than that in the ATR model group (P =0.001, P = 0.001, and P = 0.001, respectively). Furthermore, the SS level was lower in the ATR medium- and high-dosegroups than that in the ATR model group (P = 0.001 and P = 0.006). Conclusion: The PC drug pair bidirectionallyadjusted the NEO- and ATR-induced functional dyspepsia in mice by modulating GAS, VIP, and SS levels in theintestine.展开更多
Objective: To investigate the curative effect of electroacupuncture on luteinized unruptured follicle syndrome(LUFS) and its mechanism, Methods: Sixty LUFS patients were randomly allocated to treatment (electroac...Objective: To investigate the curative effect of electroacupuncture on luteinized unruptured follicle syndrome(LUFS) and its mechanism, Methods: Sixty LUFS patients were randomly allocated to treatment (electroacupuncture) group and control (hCG) group. The follicular condition and ovarian arterial flow under color Doppler ultrasonogaphy and a change in reproductive endocrine level were observed in the patients before and after treatments. The ovulation rates and pregnancy rates were compared between the two groups, and the mechanism was investigated. Results: Both ovulation rate and pregnancy rate were significantly higher in the treatment group than in the control group (P〈0.01). There was a significant difference in the improvement in ovarian arterial flow between the treatment and control groups (P〈0.01). The improvement in endocrine level was significantly more marked in the treatment group than in the control group(P〈0.01). Conclusion: Electroacupuncture has a good effect on luteinized unrupture follicle syndrome. Its mechanism may be related to the improvement in ovarian arterial blood supply and the regulation of endocrine level.展开更多
文摘Objective: To evaluate the regulatory effects of Banxia (Pinellia ternata, P) and Huanglian (Coptis chinensis, C) drugpair, derived from Banxiaxiexin soup, on the gastrointestinal movement of mice with functional dyspepsia. Methods:Mice were treated with different proportions of the P and C drug pair (1:1, 3:1, and 4:1) for 10 days, and subsequentlyinjected with atropine (ATR) or neostigmine (NEO). The effects of the different proportions of P and C were evaluatedbased on the alvine advance rate. In addition, we used the same modeling method used in the first experiment andadministered P: C at ratio of 3:1 and at different doses respectively (4.68 g/L, 2.34 g/L, and 1.17 g/L), and tested levelsof the gastrointestinal hormones, gastrin (GAS), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) in thesmall intestinal tissue using an enzyme-linked immunosorbent assay. Results: In the groups of NEO-induced mice, P:Cat ratios of 1:1, 3:1, and 4:1 significantly reduced the alvine advance rate compared with the NEO model group (P =0.003, P = 0.012 and P = 0.021, respectively). In the groups of ATR-induced mice, only P:1 at ratio of 3:1 significantlyincreased the alvine advance rate compared with the ATR model group (P = 0.007). After exposure to P: C at ratio of 3:1and at different dose, the GAS level was lower in the low-, medium-, and high-dose NEO groups than that in the NEOmodel group (P = 0.001, P = 0.004, and P = 0.003, respectively). The VIP levels were higher in the medium-andhigh-dose NEO groups than that in the NEO model group (P = 0.004 and P = 0.002, respectively). In addition, the SSlevel increased in the NEO medium-dose group compared with that in the NEO model group (P = 0.002). The GAS levelwas higher in the ATR medium- and high-dose groups than in the ATR model group (P = 0.007 and P = 0.021,respectively). The VIP level was lower in the ATR low-, medium-, and high-dose than that in the ATR model group (P =0.001, P = 0.001, and P = 0.001, respectively). Furthermore, the SS level was lower in the ATR medium- and high-dosegroups than that in the ATR model group (P = 0.001 and P = 0.006). Conclusion: The PC drug pair bidirectionallyadjusted the NEO- and ATR-induced functional dyspepsia in mice by modulating GAS, VIP, and SS levels in theintestine.
文摘Objective: To investigate the curative effect of electroacupuncture on luteinized unruptured follicle syndrome(LUFS) and its mechanism, Methods: Sixty LUFS patients were randomly allocated to treatment (electroacupuncture) group and control (hCG) group. The follicular condition and ovarian arterial flow under color Doppler ultrasonogaphy and a change in reproductive endocrine level were observed in the patients before and after treatments. The ovulation rates and pregnancy rates were compared between the two groups, and the mechanism was investigated. Results: Both ovulation rate and pregnancy rate were significantly higher in the treatment group than in the control group (P〈0.01). There was a significant difference in the improvement in ovarian arterial flow between the treatment and control groups (P〈0.01). The improvement in endocrine level was significantly more marked in the treatment group than in the control group(P〈0.01). Conclusion: Electroacupuncture has a good effect on luteinized unrupture follicle syndrome. Its mechanism may be related to the improvement in ovarian arterial blood supply and the regulation of endocrine level.