三只猴脑作冠状、地平和矢状连续切片。使用仪器为PDP11/34型电子计算机连於Evans and Suther-land图象系统Ⅱ。左、右丘脑及其14核团连续切片的轮廓,均以同样放大倍数描绘和数字转化。己数字转化的每张切片可在此计算机图象系统的屏幕...三只猴脑作冠状、地平和矢状连续切片。使用仪器为PDP11/34型电子计算机连於Evans and Suther-land图象系统Ⅱ。左、右丘脑及其14核团连续切片的轮廓,均以同样放大倍数描绘和数字转化。己数字转化的每张切片可在此计算机图象系统的屏幕上展示;并依相邻两张切片和同一丘脑其它各数字转化切片的位置关系,进行三维空间的对位。不同猴脑的核团可依照同一尺寸进行展示,并在同一三维空间登记核团。作统计学处理后,能对比左、右侧核团和三只猴丘脑各核的体积,并对不同种属动物的丘脑核团作定量比较。猴丘脑的计算机重建图象是一种可对脑内任何结构进行三维空间的立体重建和展示分析的技术。展开更多
Objective: To explore the role of abnormal neuronal activity in the basal gang lia and thalamus in the generation of dystonia. Methods: Microelectrode recordin g was performed in the globus pallidus internus (GPi), ve...Objective: To explore the role of abnormal neuronal activity in the basal gang lia and thalamus in the generation of dystonia. Methods: Microelectrode recordin g was performed in the globus pallidus internus (GPi), ventral thalamic nuclear group ventral oral posterior/ventral intermediate, Vop/Vim) and subthalamic nucl eus (STN) in patients with primary dystonia (n=11) or secondary dystonia (n=9) d uring surgery. Electromyogram (EMG) was simultaneously recorded in selected musc le groups. Single unit analysis and cross correlations were carried out. Result s: Three hundred and sixty seven neurons were obtained from 29 trajectories (GP i: 13; Vop/Vim:12; STN: 4), 87%exhibited altered neuronal activity including gr ouped discharges in GPi (n=79) and STN (n=37), long lasting neuronal activity ( n=70) and rapid neuronal discharge (n=86) in Vop/Vim. There were neurons in Vop, GPi and STN firing at the same frequency as EMG during dystonia (mean: 0.39 Hz, range 0.12-0.84 Hz). Significant correlations between neuronal activity and EM G at the frequency of dystonia were obtained (GPi: r2=0.7 (n=31), Vop/Vim: r2=0. 64 (n=18) and STN: r2=0.86 (n=17)). Conclusions: Consistent with previous findin gs of abnormalities observed in Vop/VIM and GPi in relation to dystonia, the pre sent data further show that the altered activity in GPi, specifically in dorsal subregions of GPi, Vop/Vim and STN is likely to be directly involved in the prod uction of dystonic movement. Dystonia related neuronal activity observed in mot or thalamus and basal ganglia nuclei of GPi and STN indicates a critical role of their interactions affecting both indirect and direct pathways in the developme nt of either generalized or focal dystonia. Significance: These data support a c entral role of the basal ganglia in producing dystonic movements.展开更多
文摘三只猴脑作冠状、地平和矢状连续切片。使用仪器为PDP11/34型电子计算机连於Evans and Suther-land图象系统Ⅱ。左、右丘脑及其14核团连续切片的轮廓,均以同样放大倍数描绘和数字转化。己数字转化的每张切片可在此计算机图象系统的屏幕上展示;并依相邻两张切片和同一丘脑其它各数字转化切片的位置关系,进行三维空间的对位。不同猴脑的核团可依照同一尺寸进行展示,并在同一三维空间登记核团。作统计学处理后,能对比左、右侧核团和三只猴丘脑各核的体积,并对不同种属动物的丘脑核团作定量比较。猴丘脑的计算机重建图象是一种可对脑内任何结构进行三维空间的立体重建和展示分析的技术。
文摘Objective: To explore the role of abnormal neuronal activity in the basal gang lia and thalamus in the generation of dystonia. Methods: Microelectrode recordin g was performed in the globus pallidus internus (GPi), ventral thalamic nuclear group ventral oral posterior/ventral intermediate, Vop/Vim) and subthalamic nucl eus (STN) in patients with primary dystonia (n=11) or secondary dystonia (n=9) d uring surgery. Electromyogram (EMG) was simultaneously recorded in selected musc le groups. Single unit analysis and cross correlations were carried out. Result s: Three hundred and sixty seven neurons were obtained from 29 trajectories (GP i: 13; Vop/Vim:12; STN: 4), 87%exhibited altered neuronal activity including gr ouped discharges in GPi (n=79) and STN (n=37), long lasting neuronal activity ( n=70) and rapid neuronal discharge (n=86) in Vop/Vim. There were neurons in Vop, GPi and STN firing at the same frequency as EMG during dystonia (mean: 0.39 Hz, range 0.12-0.84 Hz). Significant correlations between neuronal activity and EM G at the frequency of dystonia were obtained (GPi: r2=0.7 (n=31), Vop/Vim: r2=0. 64 (n=18) and STN: r2=0.86 (n=17)). Conclusions: Consistent with previous findin gs of abnormalities observed in Vop/VIM and GPi in relation to dystonia, the pre sent data further show that the altered activity in GPi, specifically in dorsal subregions of GPi, Vop/Vim and STN is likely to be directly involved in the prod uction of dystonic movement. Dystonia related neuronal activity observed in mot or thalamus and basal ganglia nuclei of GPi and STN indicates a critical role of their interactions affecting both indirect and direct pathways in the developme nt of either generalized or focal dystonia. Significance: These data support a c entral role of the basal ganglia in producing dystonic movements.
