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软肝颗粒对四氯化碳复合法致肝纤维化大鼠血清丙氨酸氨基转移酶等5项指标的影响
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作者 毕红征 薛敬礼 +2 位作者 黄国钧 章金涛 王纯耀 《时珍国医国药》 CAS CSCD 北大核心 2008年第12期3048-3049,共2页
目的研究软肝颗粒(HG颗粒)对CC l4(四氯化碳)复合法致肝纤维化大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总蛋白(TP)、白蛋白(ALb)及球蛋白(G lob)含量的影响。方法70只SD大鼠,除空白对照组10只外,其余各组大鼠皮下注... 目的研究软肝颗粒(HG颗粒)对CC l4(四氯化碳)复合法致肝纤维化大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总蛋白(TP)、白蛋白(ALb)及球蛋白(G lob)含量的影响。方法70只SD大鼠,除空白对照组10只外,其余各组大鼠皮下注射CC l4,同时给予高脂饲料和20%乙醇喂养6周建立肝纤维化大鼠模型,存活大鼠用分组软件按性别、体重随机分为5组,分别予HG颗6,3,1.5 g/kg 3个剂量组、复方鳖甲软肝片1.0 g/kg灌胃治疗4周。对照观察两药物对模型大鼠血清ALT,AST,TP,A lb及G lob的影响。结果模型大鼠血清ALT、AST活力显著增高,血清TP含量略升高,A lb含量显著降低,G lob含量显著升高。HG颗粒治疗后降低ALT和AST活性,降低TP和G lob含量,增加A lb含量,复方鳖甲软肝片亦可降低ALT,AST活性,降低TP和G lob含量,增加A lb含量。综合疗效HG颗粒优于复方鳖甲软肝片。结论HG颗粒能有效治疗CC l4复合法所致肝纤维化大鼠。 展开更多
关键词 HG颗粒 转移 天冬转移 总蛋白 白蛋白 球蛋白
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术前血清丙氨酸氨基转移酶与天冬氨酸氨基转移酶比值和肝癌患者预后的关系 被引量:3
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作者 辛建 张巍 《现代肿瘤医学》 CAS 北大核心 2021年第24期4357-4362,共6页
目的:探讨术前血清丙氨酸氨基转移酶与天冬氨酸氨基转移酶比值(LSR)和肝癌患者预后之间的关系。方法:回顾性分析北部战区总医院肝胆外科2014年4月至2017年4月收治的100例肝癌手术患者的临床资料。采用ROC曲线确定LSR的最佳临界值为0.903... 目的:探讨术前血清丙氨酸氨基转移酶与天冬氨酸氨基转移酶比值(LSR)和肝癌患者预后之间的关系。方法:回顾性分析北部战区总医院肝胆外科2014年4月至2017年4月收治的100例肝癌手术患者的临床资料。采用ROC曲线确定LSR的最佳临界值为0.903,以术前LSR是否大于0.903分为低LSR组、高LSR组。术后随访3年,观察生存情况,同时对于患者的临床病理相关性数据进行统计学分析,单因素生存分析选择log rank检验和Kaplan-Meier法,多因素生存分析选择Cox回归分析,最后在此基础上构建列线图,进行内部验证。结果:两组患者在性别、T分期、Edmondson分级方面差异有统计学意义(P<0.05),但是在年龄、AFP、HBsAg、白蛋白、微血管侵犯、早期复发、肿瘤直径、CTP分级、CS分期、BCLC分期、病理类型方面比较差异无统计学意义(P>0.05)。所有患者均进行3年随访,共有57例存活,生存率为57%,43例死亡,死亡率为43%。低LSR组和高LSR组患者的肿瘤进展率分别为88%(44/50)、92%(46/50),早期复发率分别为60%(30/50)、72%(36/50),中位OS分别为29.5个月、23个月,中位PFS分别为24.5个月、16个月。单因素分析结果显示:AFP、HBsAg、微血管侵犯、早期复发、CS分期、BCLC分期、LSR≤0.903是影响患者OS的因素(P<0.05)。Cox多因素分析结果显示:LSR≤0.903是影响肿瘤进展的独立预后因素(P=0.000),同时CS分期、BCLC分期对于肝癌的进展也有一定的影响(P=0.049,P=0.004)。LSR的升高可显著降低肝癌患者的OS和PFS。在内部验证中,列线图C-指数为0.751(95%CI为0.685~0.818)。结论:术前LSR是肝癌患者术后预后的独立影响因素,且与临床病理特征具有相关性,在评估肝癌患者的预后方面具有一定的临床意义。因此,在术前综合管理阶段,应尽可能恢复和改善肝功能,以获得更好的肝癌预后,延长患者的生存时间。 展开更多
关键词 肝癌 血清转移与天冬转移比值 预后 列线图 早期复发
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腹腔镜胆囊切除术后肝硬化患者转氨酶的变化
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作者 白宏宇 叶剑飞 赵国忠 《天津医药》 CAS 北大核心 2011年第8期753-754,共2页
以往的研究表明,腹腔镜胆囊切除术(laparoscopi ccholecystectomy,LC)对早期、代偿性肝硬化患者具有很高的安全性[1]。但是,LC对肝硬化患者肝功能影响如何,文献报道则很少,该类患者术后肝功能的变化规律还不明确[2-3]。本研究旨在通... 以往的研究表明,腹腔镜胆囊切除术(laparoscopi ccholecystectomy,LC)对早期、代偿性肝硬化患者具有很高的安全性[1]。但是,LC对肝硬化患者肝功能影响如何,文献报道则很少,该类患者术后肝功能的变化规律还不明确[2-3]。本研究旨在通过对比观察肝硬化及慢性乙型肝炎患者LC前后转氨酶的变化,探讨肝硬化患者LC术后肝功能变化的病理机制。 展开更多
关键词 胆囊切除术 腹腔镜肝硬化肝炎 乙型 慢性天冬转移
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天冬氨酸转氨酶与丙氨酸氨基转移酶比值和Child Pugh评分对肝硬化预后的评估 被引量:8
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作者 何益 熊伍军 《中国中西医结合消化杂志》 CAS 2005年第1期28-30,共3页
[目的] 探讨天冬氨酸转氨酶(AST)/丙氨酸氨基转移酶(ALT)比值与 Child Pugh评分对慢性乙肝肝硬化患者短、中期预后评估的价值。[方法] 对173例肝硬化患者的生存率进行回顾性评估,随访1 a。计算每例患者入院当天的AST/ALT比值与Child Pug... [目的] 探讨天冬氨酸转氨酶(AST)/丙氨酸氨基转移酶(ALT)比值与 Child Pugh评分对慢性乙肝肝硬化患者短、中期预后评估的价值。[方法] 对173例肝硬化患者的生存率进行回顾性评估,随访1 a。计算每例患者入院当天的AST/ALT比值与Child Pugh评分,以受试者特征曲线(ROC)下的面积衡量两者预测患者预后的能力,分析AST/ALT比值与Child Pugh评分的相关性。[结果] AST/ALT比值与 Child Pugh 评分呈显著相关( r=0 53,P<0 01)。随访3个月及1 a内分别有30例及50例患者死亡,死亡者AST/ALT比值与 Child Pugh 评分显著高于生存者(P<0 01)。依据 ROC曲线 AST/ALT比值截断值为 1 6,对患者 1 a预后判断的敏感性为55%,特异性为72%;Child Pugh 评分截断值为10,其敏感性76%,特异性 68%,联合应用两者敏感性和特异性可分别提高至85% 和76%。[结论] AST/ALT比值及Child Pugh评分均可有效地预测肝硬化患者的短期和中期预后,联合应用可提高其准确率。 展开更多
关键词 肝硬化 天冬/转移比值 CHILD Pugh评分 预后
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ALT/AST及影像学特征预测肝癌经动脉化疗栓塞患者的预后 被引量:1
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作者 李大伟 周振堰 +3 位作者 周长友 张宁平 尚海龙 王一超 《介入放射学杂志》 CSCD 北大核心 2024年第8期849-854,共6页
目的探讨经动脉化疗栓塞(transarterial chemoembolization,TACE)治疗前血清丙氨酸转氨酶/天冬氨酸转氨酶比值(ALT/AST)及影像学特征与肝癌患者预后的关系,构建预测患者总体生存率(overall survival,OS)的诺模图模型。方法纳入2016年7月... 目的探讨经动脉化疗栓塞(transarterial chemoembolization,TACE)治疗前血清丙氨酸转氨酶/天冬氨酸转氨酶比值(ALT/AST)及影像学特征与肝癌患者预后的关系,构建预测患者总体生存率(overall survival,OS)的诺模图模型。方法纳入2016年7月至2020年7月苏州大学附属第一医院广慈分院诊断为肝癌并以TACE作为初始治疗的患者211例,将其随机分为建模组139例,验证组72例。采用受试者工作特征(receiver operation characteristics,ROC)曲线确定AST/ALT的最佳临界值。在建模组患者中进行单、多因素Cox回归分析,筛选影响肝癌患者OS的独立预测因素并构建预后模型。通过Harrell一致性指数(C指数)评价诺模图对肝癌患者OS的预测能力,校准曲线用于评估预后模型的预测准确性。结果建模组与验证组患者的基线特征分布差异均无统计学意义(均P>0.05)。两组患者中位OS分别为28.5个月(95%CI:22.1~34.9)和25.1个月(95%CI:19.2~29.0),差异无统计学意义(χ^(2)=1.395,P=0.322)。AST/ALT预测肝癌患者OS的最佳临界值为1.10,曲线下面积(area under curve,AUC)为0.674(95%CI:0.604~0.753)。Cox回归分析表明,肿瘤个数(HR=2.080,95%CI:1.245~3.475,P=0.005)、肿瘤包膜(HR=1.771,95%CI:1.128~2.780,P=0.013)、不规则边缘强化(HR=1.884,95%CI:1.190~2.984,P=0.007)和AST/ALT(HR=2.450,95%CI:1.506~3.987,P<0.01)是接受TACE治疗肝癌患者的独立预后因素。基于以上变量构建预测OS的诺模图模型,其在建模组与验证组中的C指数分别为0.733(95%CI:0.650~0.826)和0.770(95%CI:0.688~0.862)。校准曲线显示预后模型对1、2和3年OS的预测曲线与理想参考线之间未见明显偏离。结论基于肿瘤个数、影像学特征及AST/ALT的诺模图对接受TACE治疗的肝癌患者预后显示了良好的预测价值。 展开更多
关键词 肝癌 经动脉化疗栓塞 转移/天冬转移比值 预后 诺模图
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无创模型评估慢乙肝肝纤维化的临床价值 被引量:1
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作者 徐旭 赵雪珂 陈有望 《贵州医科大学学报》 CAS 2024年第4期620-624,共5页
目的探究无创模型评估慢乙肝肝纤维化程度的临床使用价值。方法收集经肝穿诊断为肝纤维化的92例慢乙肝患者资料,根据肝纤维化程度将患者分为轻度肝纤维化组和显著肝纤维化组,采用全自动生化分析仪检测2组患者静脉血天门冬氨酸氨基转移酶... 目的探究无创模型评估慢乙肝肝纤维化程度的临床使用价值。方法收集经肝穿诊断为肝纤维化的92例慢乙肝患者资料,根据肝纤维化程度将患者分为轻度肝纤维化组和显著肝纤维化组,采用全自动生化分析仪检测2组患者静脉血天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT),血细胞分析仪检测2组患者静脉血血小板计数(PLT)及红细胞分布宽度(RDW),计算AST与ALT比值(AAR)、AST与PLT比值(APRI)、RDW与PLT比值(RPR)及4因子肝纤维化指数(FIB-4);应用受试者工作特征曲线(ROC)曲线下面积(AUC)评价上述无创模型对肝纤维化的诊断价值。结果轻度纤维化组血清ALT与AST、APRI、RPR以及FIB-4均低于显著纤维化组(P<0.05),PLT和AAR则高于显著纤维化组(P<0.05);应用AAR、APRI、RPR和FIB-4评估慢性乙型肝炎肝纤维化程度的AUC分别为0.406、0.711、0.729和0.692;4项指标联合诊断的AUC为0.741,显著高于4项中的任何1项单独指标(P<0.05)。结论APRI、RPR和FIB-4是诊断慢乙肝患者肝纤维化程度的有效指标,4种模型联合应用可提高乙肝肝纤维化程度诊断和预测的准确性。 展开更多
关键词 慢性乙型肝炎 肝纤维化 无创诊断模型 天门冬转移/转移比值 天门冬转移/血小板比值 红细胞分布宽度/血小板比值 FIB-4指数
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FAR、CAR、LSR、甲胎蛋白水平及微血管侵犯与原发性肝癌患者TACE术后生存时间的关系 被引量:2
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作者 马庆霞 顾澄 +1 位作者 严铃铃 张弛 《国际检验医学杂志》 CAS 2023年第13期1599-1603,共5页
目的研究纤维蛋白原/清蛋白比值(FAR)、C反应蛋白/清蛋白比值(CAR)、丙氨酸氨基转移酶/天冬氨酸氨基转移酶比值(LSR)、甲胎蛋白(AFP)水平及微血管侵犯与原发性肝癌患者经导管动脉栓塞化疗(TACE)术后生存时间的关系。方法将南通市肿瘤医... 目的研究纤维蛋白原/清蛋白比值(FAR)、C反应蛋白/清蛋白比值(CAR)、丙氨酸氨基转移酶/天冬氨酸氨基转移酶比值(LSR)、甲胎蛋白(AFP)水平及微血管侵犯与原发性肝癌患者经导管动脉栓塞化疗(TACE)术后生存时间的关系。方法将南通市肿瘤医院2016年1月至2017年1月收治的93例原发性肝癌患者纳入研究。对患者进行5年随访,根据患者的中位生存时间(45.98个月)将患者分为生存时间≤45.98个月组和生存时间>45.98个月组。比较两组患者FAR、CAR、LSR、AFP水平,微血管侵犯情况及其他临床病理特征,分析原发性肝癌患者TACE术后生存时间较短的独立危险因素,同时绘制K-M生存曲线分析不同FAR、CAR、LSR、AFP水平患者的生存时间。结果生存时间≤45.98个月组存在肝外转移、存在微血管侵犯、肿瘤低分化者的比例以及FAR、CAR、LSR、AFP水平均高于生存时间>45.98个月组(P<0.05)。原发性肝癌患者存在肝外转移、存在微血管侵犯、肿瘤低分化及高FAR、CAR、LSR、AFP水平均是患者TACE术后生存时间缩短的独立危险因素(P<0.05)。低FAR、CAR、LSR、AFP水平患者5年生存率分别高于高FAR、CAR、LSR、AFP水平患者(P<0.05)。结论FAR、CAR、LSR、AFP及微血管侵犯均是原发性肝癌患者TACE术后生存时间较短的独立危险因素。 展开更多
关键词 纤维蛋白原/清蛋白比值 C反应蛋白/清蛋白比值 转移/天冬转移比值 微血管侵犯 经导管动脉栓塞化疗 预后
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GPR、FIB–4及AAR对自身免疫性肝病患者早期肝硬化的诊断价值
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作者 石林丽 赵俊岭 刘鑫 《深圳中西医结合杂志》 2023年第12期79-81,F0003,共4页
目的:探讨γ-谷酰胺转肽酶与血小板比值(GPR)、纤维化指数-4(FIB-4)、天门冬氨酸氨基转移酶与丙氨酸氨基转移酶比值(AAR)对自身免疫性肝病患者早期肝硬化的诊断价值。方法:选择2019年10月至2022年10月于邓州市中心医院就诊的60例自身免... 目的:探讨γ-谷酰胺转肽酶与血小板比值(GPR)、纤维化指数-4(FIB-4)、天门冬氨酸氨基转移酶与丙氨酸氨基转移酶比值(AAR)对自身免疫性肝病患者早期肝硬化的诊断价值。方法:选择2019年10月至2022年10月于邓州市中心医院就诊的60例自身免疫性肝炎患者,将其分为肝硬化组和非硬化组,分别为23例、37例。比较两组患者的GPR、FIB-4、AAR水平,采用受试者工作特征曲线(ROC)分析GPR、FIB-4、AAR对自身免疫性肝病患者早期肝硬化的诊断价值。结果:肝硬化组患者的GPR、FIB-4、AAR水平均比非肝硬化组患者高,差异均具有统计学意义(P<0.05);GPR、FIB-4、AAR对自身免疫性肝病患者早期肝硬化进行诊断的曲线下面积(AUC)值分别为0.977、0.929、0.765,而联合上述指标诊断的AUC值为0.994,显著高于各指标单独检测,差异具有统计学意义(P<0.05)。结论:GPR、FIB-4、AAR应用于自身免疫性肝病患者早期肝硬化的诊断中的临床价值较高,联合检测上述指标的价值高于单独检测。 展开更多
关键词 自身免疫性肝病 早期肝硬化 γ-谷酰胺转肽与血小板比值 纤维化指数-4 天门冬转移转移比值
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HBsAg定量检测在慢性乙型病毒性肝炎患者诊疗中的临床意义 被引量:13
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作者 卢建华 杨莉 +3 位作者 叶立红 赵召霞 许怡 戴二黑 《现代中西医结合杂志》 CAS 2020年第33期3674-3678,共5页
目的通过分析HBV表面抗原(HBsAg)的表达量与乙型肝炎病毒DNA(HBV DNA)、乙型肝炎e抗原(HBeAg)及丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的关系,探讨HBsAg表达量在慢性乙型病毒性肝炎(慢乙肝)患者诊疗中的意义。方法收集2012... 目的通过分析HBV表面抗原(HBsAg)的表达量与乙型肝炎病毒DNA(HBV DNA)、乙型肝炎e抗原(HBeAg)及丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的关系,探讨HBsAg表达量在慢性乙型病毒性肝炎(慢乙肝)患者诊疗中的意义。方法收集2012年12月—2015年12月在石家庄市第五医院就诊的慢乙肝患者624例,采用酶免疫发光技术检测血清HBsAg及HBeAg水平,根据HBsAg值将患者分为0~50 IU/mL组、50~1000 IU/mL组、1000~5000 IU/mL组、5000~10000 IU/mL组、10000~25000 IU/mL组及>25000 IU/mL组,采用荧光定量PCR技术检测各组HBV DNA表达量,采用全自动生化仪检测各组血清ALT、AST水平,分析HBsAg不同定量值的临床意义。结果随着患者血清中HBsAg水平不断增高,HBV DNA表达量及HBeAg阳性率亦显示增高趋势,10000~25000 IU/mL组与>25000 IU/mL组的HBV DNA表达量及HBeAg阳性率比0~50 IU/mL组、50~1000 IU/mL组、1000~5000 IU/mL组、5000~10000 IU/mL组明显增高,差异均有统计学意义(P均<0.05)。HBeAg阳性患者血清HBsAg水平比HBeAg阴性患者明显增高(P<0.05)。各组ALT、AST水平呈异常状态,只有HBsAg>25000 IU/mL组的患者ALT、AST水平多数在正常范围。结论随着HBsAg水平不断增高,患者体内HBV复制活跃,且病毒数量不断增多;而HBsAg高于25000 IU/mL的患者多数处于免疫耐受期,位于1000~5000 IU/mL的患者多数处于低复制期,这部分患者均需密切监视,有利于疾病及时治疗。 展开更多
关键词 慢性乙型肝炎 乙肝表面抗原 乙型肝炎病毒DNA 转移天门冬转移
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慢性乙型肝炎患者血清IL-18的检测与临床价值 被引量:1
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作者 刘林 冯成全 《成都医学院学报》 CAS 2012年第4期632-634,共3页
目的探讨血清白介素18(IL-18)对慢性乙型肝炎(CHB)疾病活动度的影响。方法检测CHB患者血液中ALT、AST、IL-18、乙型肝炎血清学标志物及HBeAg定量、HBVDNA等,并统计分析。结果在CHB患者中,HBeAg阴性组患者血清ALT、AST均较抗HBe阳性组、H... 目的探讨血清白介素18(IL-18)对慢性乙型肝炎(CHB)疾病活动度的影响。方法检测CHB患者血液中ALT、AST、IL-18、乙型肝炎血清学标志物及HBeAg定量、HBVDNA等,并统计分析。结果在CHB患者中,HBeAg阴性组患者血清ALT、AST均较抗HBe阳性组、HBeAg阳性组、正常对照组明显升高(P<0.05),IL-18含量明显升高(P<0.01);HBeAg阴性组患者血清ALT异常率63.6%、IL-18异常率86.4%,二者比较差异有明显统计学意义(P<0.05)。HBeAg阳性组与正常对照组及抗HBe阳性组比较,ALT、AST均明显增高(P<0.05);HBeAg阳性组与HBeAg阴性组比较,HBV DNA拷贝量、HBeAg定量(S/CO值)明显较高(P<0.01,P<0.001);IL-18水平明显较低(P<0.01);HBeAg水平与IL-18水平呈明显负相关关系(r=-0.536、P<0.01),HBeAg阴性组,HBeAg与IL-18水平无明显相关性(r=-0.227、P>0.05)。结论 CHB患者中,HBeAg对机体的免疫应答功能、疾病活动度有明显的影响。IL-18参与了HBV感染后的免疫反应、肝脏损伤过程,且血清IL-18水平与肝损伤程度相关。 展开更多
关键词 肝炎 乙型 慢性 HBeAg HBVDNA IL-18 转移 天门冬转移
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供血(浆)员3110名抗-HCV检测结果分析
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作者 董钧铭 张云东 +2 位作者 滕敏 李隆昌 唐文凯 《贵州医药》 CAS 1996年第4期209-211,共3页
丙型腺炎病毒(HCV)系经血传播。为探讨献血人群中HCV感染情况,我们用酶联免疫吸附法(ELISA)对3110名献血员每次献血(浆)前作抗—HCV调查.结果.抗—HCV阳性检出率在供全血的献血员为。96%,在供装员中随献装次数增多,由0.19... 丙型腺炎病毒(HCV)系经血传播。为探讨献血人群中HCV感染情况,我们用酶联免疫吸附法(ELISA)对3110名献血员每次献血(浆)前作抗—HCV调查.结果.抗—HCV阳性检出率在供全血的献血员为。96%,在供装员中随献装次数增多,由0.19%逐月上升至目前的15.55%,同时.我们对抗—HCV阳性的供血(浆)者进行血清丙氨酸氨基转移酶(ALT)测定观察发现.抗—HCV阳性的献血员大多有ALT不同程度升高.ALT水平高低可能还与HCV在体内复制活跃有关。 展开更多
关键词 型肝炎病毒 献血员 ELISA 丙氨酸氨转移酶
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应用瞬时弹性成像技术联合APRI和AAR指数评估慢性乙型肝炎患者肝纤维化临床价值研究 被引量:13
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作者 代海峰 王甜 +2 位作者 雷迅 蔡佳 黄文祥 《实用肝脏病杂志》 CAS 2021年第2期196-199,共4页
目的探讨应用瞬时弹性成像技术联合天门冬氨酸氨基转移酶(AST)/血小板指数(APRI)和AST/丙氨酸氨基转移酶(ALT)比值(AAR)评估慢性乙型肝炎(CHB)患者肝纤维化的临床价值。方法2017年5月~2018年5月我院收治的CHB患者118例,给予所有患者恩... 目的探讨应用瞬时弹性成像技术联合天门冬氨酸氨基转移酶(AST)/血小板指数(APRI)和AST/丙氨酸氨基转移酶(ALT)比值(AAR)评估慢性乙型肝炎(CHB)患者肝纤维化的临床价值。方法2017年5月~2018年5月我院收治的CHB患者118例,给予所有患者恩替卡韦治疗观察12个月,治疗前接受肝活检,使用法国爱科森公司生产的FibroScan-502型瞬时弹性扫描仪行肝脏硬度检测(LSM),应用受试者工作特征曲线(ROC)评价LSM、APRI和AAR诊断CHB患者大于或等于S2期(显著)肝纤维化的效能。结果在本组118例CHB患者中,经肝组织病理学检查,诊断S0~1期(无肝纤维化组)65例,S_(2~4)期(显著肝纤维化组)53例;显著肝纤维化组血清ALT、AST、APRI、AAR和LSM分别为(128.3±31.4)U/L、(133.7±41.9)U/L、(0.5±0.2)、(1.5±0.5)和(9.3±2.7)kPa,与无肝纤维化组比,差异显著【分别为(104.6±26.8)U/L、(93.4±40.2)U/L、(0.3±0.1)、(1.2±0.3)和(5.6±1.6)kPa,P<0.05】,而外周血血小板计数为(123.4±46.4)×10^(9)/L,显著低于无肝纤维化组【(184.2±42.8)×10^(9)/L,P<0.05】;分别以7.830(kPa)、0.350和1.310为截断点,应用LSM、APRI和AAR诊断CHB患者显著肝纤维化的AUC分别为0.867(95%CI:0.827~0.947)、0.774(95%CI:0.678~0.861)和0.687(95%CI:0.500~0.774),三项指标联合诊断AUC为0.945(95%CI:0.904~0.986),显著高于任何一项单独指标(P<0.05);65例无显著肝纤维化患者治疗前后LSM、APRI和AAR变化不显著(P>0.05),而53例显著肝纤维化患者在治疗12个月后LSM为(7.4±1.2)kPa,显著低于治疗前【(9.3±2.7)kPa,P<0.05】。结论应用瞬时弹性成像技术联合APRI和AAR检测CHB患者显著肝纤维化有一定的临床价值,值得进一步观察和研究。 展开更多
关键词 慢性乙型肝炎 肝纤维化 瞬时弹性成像技术 天门冬转移/血小板比值 天门冬转移/转移比值 诊断
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Dynamic tracking of stem cells in an acute liver failure model 被引量:12
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作者 Tarek Ezzat Dipok Kumar Dhar +1 位作者 Massimo Malago Steven WM Olde Damink 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第6期507-516,共10页
AIM: To investigate a dual labeling technique, which would enable real-time monitoring of transplanted em- bryonic stem cell (ESC) kinetics, as well as long-term tracking. METHODS: Liver damage was induced in C57/... AIM: To investigate a dual labeling technique, which would enable real-time monitoring of transplanted em- bryonic stem cell (ESC) kinetics, as well as long-term tracking. METHODS: Liver damage was induced in C57/BL6 male mice (n = 40) by acetaminophen (APAP) 300 mg/kg administered intraperitoneally. Green fluores- cence protein (GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbo- cyanine iodide (DiR) immediately before transplantationinto the spleen. Each of the animals in the cell therapy group (n = 20) received 5 x 106 ESCs 4 h following treatment with APAP. The control group (n = 20) re- ceived the vehicle only. The distribution and dynamics of the cells were monitored in real-time with the IVIS lumina-2 at 30 rain post transplantation, then at 3, 12, 24, 48 and 72 h, and after one and 2 wk. Immunohisto- chemical examination of liver tissue was used to identify expression of GFP and albumin. Plasma alanine amino- transferase (ALT) was measured as an indication of liver damage.RESULTS: DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence. The transplanted cells were confined inside the spleen at 30 min post-transplantation, gradu- ally moved into the splenic vein, and were detectable in parts of the liver at the 3 h time-point. Within 24 h of transplantation, homing of almost 90% of cells was confirmed in the liver. On day three, however, the DiR signal started to fade out, and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imag- ing, and confirmed that the highest photon emission was in the liver (P 〈 0.0001). At 2 wk, the DiRsignal was no longer detectable in vivo; however, immuno- histochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells. GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma, with the presence of a larger number of GFP +ve cells incorporated within the sinu- soidal endothelial lining. Very faint albumin expression was detected in the transplanted GFP +re cells at 72 h; however at 2 wk, few cells that were positive for GFP were also strongly positive for albumin. There was a significant improvement in serum levels of ALT, albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point. In the cell therapy group, serum ALT was significantly (P = 0.016) lower and al- bumin (P = 0.009) was significantly higher when com- pared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups. CONCLUSION: Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribu- tion, survival and engraftment of transplanted cells, and could be used for cell therapy models. 展开更多
关键词 Cell transplantation Cell tracking Embry-onic stem cells Acute liver failure Liver regeneration
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Decreased mitochondrial deoxyribonucleic acid and increased oxidative damage in chronic hepatitis C 被引量:4
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作者 Hsu-Heng Yen Kai-Lun Shih +3 位作者 Ta-Tsung Lin Wei-Wen Su Maw-Soan Soon Chin-San Liu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第36期5084-5089,共6页
AIM: To determine whether alteration of the mito- chondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA△CT) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)- in... AIM: To determine whether alteration of the mito- chondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA△CT) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)- infected patients. METHODS: This study enrolled two groups of pa- tients aged 40-60 years: a control group and an HCV- infected group in Department of Gastroenterology and Hepatology in Changhua Christian Hospital. Patients with co-infection with hepatitis B virus or human im- munodeficiency virus, autoimmune disease, malignant neoplasia, pregnancy, thyroid disease, or alcohol con- sumption 〉 40 g/d were excluded. HCV-infected pa- tients who met the following criteria were included: (1) positive HCV antibodies for 〉 6 mo; (2) alanine aminotransferase (ALT) levels more than twice the upper lim- it of normal on at least two occasions during the past 6 mo; and (3) histological fibrosis stage higher than F1. The mtDNA copy number and oxidative damage index of HCV mtDNA (mtDNA△CT) were measured in periph- eral blood leukocytes. The association between mtDNA copy number and mtDNA△CT was further analyzed using clinical data. RESULTS: Forty-seven normal controls (male/female: 26/21, mean age 50.51 ± 6.15 years) and 132 HCV- infected patients (male/female: 76/61, mean age 51.65 ± 5.50 years) were included in the study. The geno- types of HCV-infected patients include type 1a (n = 3), type 1b (n = 83), type 2a (n = 32), and type 2b (n = 14). Liver fibrosis stages were distributed as follows: F1/F2/F3/F4 = 1/61/45/25 and activity scores were A0/ A1/A2/A3 = 7/45/55/25. There were no age or gender differences between the two groups. HCV-infected pa- tients had higher hepatitis activity (aspartate transami- nase levels 108.77 ± 60.73 vs 23.19 ± 5.47, P 〈 0.01; ALT levels 168.69 ± 93.12 vs 23.15 ± 9.45, P 〈 0.01) and lower platelet count (170.40±58.00 vs 251.24 ± 63.42, P 〈 0.01) than controls. The mtDNA copy num- ber was lower in HCV-infected patients than in controls (173.49 vs 247.93, P 〈 0.05). The mtDNA△CT was higher in HCV-infected patients than in controls (2.92 vs 0.64, P 〈 0.05). To clarify the clinical significance of these results in HCV-infected patients, their association with different clinical parameters among HCV-infected pa- tients was analyzed. A negative association was found between mtDNA copy number and elevated aspartate transaminase levels (r = -0.17, P 〈 0.05). Changes in mtDNA copy number were not associated with HCV RNA levels, HCV genotypes, liver fibrosis severity, or inflammatory activity in the liver biopsy specimen. How- ever, a correlation was observed between mtDNA△CT and platelet count (r = -0.22, P 〈 0.01), HCV RNA level (r = 0.36, P 〈 0.01), and hepatitis activity (r = 0.20, P = 0.02). However, no difference in the change in mtDNA△CT was observed between different fibrosis stages or HCV CONCLUSION: Oxidative stress and mtDNA dam- age are detectable in patient's peripheral leukocytes. Increased leukocyte mtDNA△CT correlates with higher HCV viremia, increased hepatitis activity, and lower platelet count. 展开更多
关键词 Hepatitis C MITOCHONDRIA Oxidative stress Mitochondrial DNA BIOMARKER
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Serum γ-glutamyltransferase,alanine aminotransferase,and aspartate aminotransferase activity in Iranian healthy blood donor men 被引量:8
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作者 Hossein Khedmat Farahnaz Fallahian +7 位作者 Hassan Abolghasemi Bashir Hajibeigi Zohre Attarchi Farshid Alaeddini Mohammad Taghi Holisaz Masoumeh Pourali Shahin Sharifi Nasrin Zarei 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第6期889-894,共6页
AIM: To determine serum γ-glutamyltransferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity, and to assess their correlation with demographic and clinical findings in healthy bl... AIM: To determine serum γ-glutamyltransferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity, and to assess their correlation with demographic and clinical findings in healthy blood donors. METHODS: This cross-sectional study was performed in 934 male blood donors, aged 18 to 68 years, who consecutively attended Tehran blood transfusion service in 2006. All participants were seronegative for HBV or HCV infections, non alcohol users, and all underwent a standard interview and anthropometric tests. Clinical and biochemical parameters including AST, ALT, and GGT activities were determined. Patients taking drugs known to cause hepatic fat deposition were excluded. For AST, ALT, and GGT variables, we used 33.33 and 66.66 percentiles, so that each of them was divided into three tertiles. RESULTS: Mean AST, ALT, and GGT activities were 25.26 ± 12.58 U/L (normal range 5-35 U/L), 33.13 ± 22.98 (normal range 5-35 U/L), and 25.11 ± 18.32 (normal range 6-37 U/L), respectively. By univariate analyses, there were significant associations between increasing AST, ALT, or GGT tertiles and age, body weight, body mass index, and waist and hip circumferences (P < 0.05). By multiple linear regression analyses, ALT was found to be positively correlated with dyslipidemia (B = 6.988, P = 0.038), whereas ALT and AST were negatively correlated with age. AST, ALT, and GGT levels had positive correlation with family history of liver disease (B = 15.763, P < 0.001), (B = 32.345, P < 0.001), (B =24.415, P < 0.001), respectively.CONCLUSION: Although we did not determine the cutoffs of the upper normal limits for AST, ALT, and GGT levels, we would suggest screening asymptomatic patients with dyslipidemia and also subjects with a family history of liver disease. 展开更多
关键词 γ-glutamyltransferase Alanine aminotransrerase Aspartate aminotransferase Blood donor
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Metformin prevents hormonal and metabolic disturbances and 1,2-dimethylhydrazine-induced colon carcinogenesis in non-diabetic rats 被引量:2
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作者 Viktoria V.Bekusova Vasily M.Patsanovskii +3 位作者 Alexander D.Nozdrachev Alexandr P.Trashkov Margarita R.Artemenko Vladimir N.Anisimov 《Cancer Biology & Medicine》 SCIE CAS CSCD 2017年第1期100-107,共8页
Effects of two doses of the anti-diabetic drug, metformin (MF), on hormonal and metabolic levels of serum of non-diabetic male Wistar rats with 1,2-dimethylhydrazine (DMH)-induced colon tumor adenocarcinomas were ... Effects of two doses of the anti-diabetic drug, metformin (MF), on hormonal and metabolic levels of serum of non-diabetic male Wistar rats with 1,2-dimethylhydrazine (DMH)-induced colon tumor adenocarcinomas were studied. Carcinogenesis in the animals was also observed. Rats with DMH-induced colon adenocarcinomas had elevated levels of serum glucose, insulin, insulin- like growth factor-l, total cholesterol, triglycerides, catalase, malonic dialdehyde, glycated hemoglobin, aspartate aminotransferase, and alanine aminotransferase and decreased hemoglobin. Treatment with two doses of MF normalized maiority of these changes in DMH-treated rats, whereas the drug was ineffective in rats without DMH treatment. The only exception was the decreased triglyceride levels in MF-treated rats. A 100 mg/kg dose of MF increased DMH-induced exophytic colon carcinomas and decreased endophytic tumors compared with untreated rats. Moreover, both MF doses increased DMH-induced and highly differentiated tumors and decreased the invasiveness of colon carcinomas compared with rats provided with DMH and water. Therefore, effects of MF on metabolic homeostasis are critical for preventing colon cancer. 展开更多
关键词 Colon cancer PREVENTION 1 2-DIMETHYLHYDRAZINE mefformin RAT
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Intrahepatic expression of genes related to metabotropic receptors in chronic hepatitis 被引量:2
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作者 Andrzej Ciesla Maciej Kusmider +6 位作者 Agata Faron-Górecka Marta Dziedzicka-Wasylewska Monika Bocisga-Jasik Danuta Owczarek Irena Cieko-Michalska Dorota Cibor Tomasz Mach 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第31期4156-4161,共6页
AIM: To screen for genes related to metabotropic re- ceptors that might be involved in the development of chronic hepatitis. METHODS: Assessment of 20 genes associated with metabotropic receptors was performed in li... AIM: To screen for genes related to metabotropic re- ceptors that might be involved in the development of chronic hepatitis. METHODS: Assessment of 20 genes associated with metabotropic receptors was performed in liver speci- mens obtained by punch biopsy from 12 patients with autoimmune and chronic hepatitis type B and C. For this purpose, a microarray with low integrity grade and with oligonucleotide DNA probes complementary to target transcripts was used. Evaluation of gene expression was performed in relation to transcript level, correlation between samples and grouping of clinical parameters used in chronic hepatitis assessment. Clini- cal markers of chronic hepatitis included alanine and aspartate aminotransferase, ~,-glutamyltranspeptidase, alkaline phosphatase and cholinesterase activity, levels of iron ions, total cholesterol, triglycerides, albumin, glucose, hemoglobin, platelets, histological analysis of inflammatory and necrotic status, fibrosis according to METAVIR score, steatosis, as well as anthropometric body mass index, waist/hip index, percentage of adi- pose tissue and liver size in ultrasound examination. Gender, age, concomitant diseases and drugs were also taken into account. Validation of oligonucleotide microarray gene expression results was done with the use of quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: The highest (0.002 〈 P 〈 0.046) expres- sion among genes encoding main components of metabotropic receptor pathways, such as the a subunit of G-coupled protein, phosphoinositol-dependent pro- tein kinase or arrestin was comparable to that of an- giotensinogen synthesized in the liver. Carcinogenesis suppressor genes, such as chemokine ligand 4, tran- scription factor early growth response protein 1 and lysophosphatidic acid receptor, were characterized by the lowest expression (0.002 〈 P 〈 0.046), while the factor potentially triggering hepatic cancer, transcrip- tion factor JUN-B, had a 20-fold higher expression. The correlation between expression of genes of protein kinases PDPK1, phosphoinositide 3-kinase and protein kinase A (Spearman's coefficient range: 0.762-0.769) confirmed a functional link between these enzymes. Gender (P = 0.0046) and inflammation severity, mea- sured by alanine aminotransferase activity (P = 0.035), were characterized by diverse metabotropic receptor gene expression patterns. The Pearson's coefficient ranging from -0.35 to 0.99 from the results of qRT-PCR and microarray indicated that qRT-PCR had certainlimitations as a validation tool for oligonucleotide mi- croarray studies. CONCLUSION: A microarray-based analysis of hepa- tocyte metabotropic G-protein-related gene expression can reveal the molecular basis of chronic hepatitis. 展开更多
关键词 Metabotropic receptors Gene expression DNA oligonucleotides Quantitative real-time poly-merase chain reaction Chronic hepatitis
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PCA-决策树模型评价miR-30a与RPR、AAR、FIB-4、APRI在乙肝患者肝纤维化的应用价值 被引量:5
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作者 张明 祝再然 +1 位作者 赵桂金 董嘉良 《广东医学》 CAS 2021年第1期106-110,共5页
目的应用主成分分析(principal component analysis, PCA)-决策树模型评价miR-30a与红细胞体积分布宽度与血小板比值(red cell volume distribution width-platelet ratio, RPR)、天冬氨酸/丙氨酸氨基转移酶比率(aspartate to alanine a... 目的应用主成分分析(principal component analysis, PCA)-决策树模型评价miR-30a与红细胞体积分布宽度与血小板比值(red cell volume distribution width-platelet ratio, RPR)、天冬氨酸/丙氨酸氨基转移酶比率(aspartate to alanine aminotransferase ratio, AAR)、FIB-4(fibrosis-4)、天冬氨酸氨基转移酶/血小板比值指数(aspartate aminotransferase/platelet ratio index, APRI)对乙型肝炎(乙肝)患者肝纤维化诊断和疾病严重程度预测的价值。方法选取收治的肝癌423例,肝良性病变293例,肝纤维化89例(合并乙肝39例),胆结石70例,肝脏活检确认病理学分级,检测血清miR-30a及血清学指标,计算RPR、AAR、FIB-4、APRI等诊断模型。采用受试者工作特征(ROC)曲线、PCA-决策树模型评价各模型的诊断和预测价值。结果肝癌、肝良性病变、肝纤维化、胆结石患者RPR、AAR、FIB-4、APRI、miR-30a比较差异有统计学意义(P<0.05),肝纤维化组RPR、FIB-4、APRI高于其余组(P<0.05),AAR、miR-30a低于其余组(P<0.05);RPR与肝纤维化、FIB-4与肝纤维化、APRI与肝纤维化均呈正相关(P<0.05),AAR与肝纤维化、miR-30a与肝纤维化均呈负相关(P<0.05);乙肝纤维化组RPR、FIB-4、APRI高于非乙肝纤维化组(P<0.05),AAR、miR-30a低于非乙肝纤维化组(P<0.05);乙肝纤维化分级与RPR、FIB-4、APRI呈正相关,与AAR、miR-30a呈负相关(P<0.05);ROC曲线下面积miR-30a最大,决策树模型中miR-30a与结果关联最强,决策树、PCA-决策树模型对乙肝纤维化的诊断准确度分别为85.3%、92.1%,预测准确度为88.5%、93.4%。结论 miR-30a与RPR、AAR、FIB-4、APRI是诊断乙肝纤维化的有效指标,也可用于乙肝纤维化严重程度判断,PCA-决策树模型可提高乙肝纤维化诊断和预测的准确度。 展开更多
关键词 乙型肝炎 主成分分析-决策树模型 肝脏纤维化 微小RNA-30a 红细胞体积分布宽度与血小板比值 天冬/转移比率 转移/血小板比值指数 FIB-4
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井下矿工肝功能情况分析 被引量:2
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作者 刘星花 《中国现代药物应用》 2011年第7期20-21,共2页
目的探讨矿工血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和r-谷氨酰基转移酶(GGT)水平状况,了解矿工肝功受损状况。方法用东芝-40全自动生化分析仪检测体检者血清ALT、AST、GGT,比较2009年和2010年我厂矿工肝功能损伤情况。结... 目的探讨矿工血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和r-谷氨酰基转移酶(GGT)水平状况,了解矿工肝功受损状况。方法用东芝-40全自动生化分析仪检测体检者血清ALT、AST、GGT,比较2009年和2010年我厂矿工肝功能损伤情况。结果 2009年肝功能异常者占16.7%,2010年占18.7%;对比两年矿工肝功能情况发现三个指标水平均有所升高,其中,2010年GGT异常率显著高于2009年,(P<0.05)。结论由于矿工的长期饮酒和不健康的饮食结构导致了肝功能的损害,建议矿工改善饮食结构,增强身体健康。 展开更多
关键词 转移天冬 转移 r-谷酰基转移 肝功能
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血清AST/ALT比值,γ-GT,ALP及AFP联合检测对原发性肝癌的诊断价值 被引量:5
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作者 李瑞芳 《临床医药实践》 2015年第10期757-758,792,共3页
目的:探讨血清天冬氨酸氨基转移酶(AST)/丙氨酸氨基转移酶(ALT)的比值、r谷氨酰转移酶(γ-GT)与碱性磷酸酶(ALP)联合血清甲胎蛋白(AFP)测定对早期原发性肝癌的诊断价值。方法:选择2012年1月—2014年8月本院收治的原发性肝癌患者94例,随... 目的:探讨血清天冬氨酸氨基转移酶(AST)/丙氨酸氨基转移酶(ALT)的比值、r谷氨酰转移酶(γ-GT)与碱性磷酸酶(ALP)联合血清甲胎蛋白(AFP)测定对早期原发性肝癌的诊断价值。方法:选择2012年1月—2014年8月本院收治的原发性肝癌患者94例,随机抽取同期本院收治的肝硬化患者44例,肝炎患者40例,均检测血清AST/ALT、γ-GT,ALP及AFP水平。结果 :肝癌组AST/ALT,ALP,γ-GT及AFP血清检测水平明显高于其他两组(P<0.05),肝癌组AST/ALT,ALP,γ-GT及AFP及AFP的阳性检出率明显高于其他两组(P<0.05)。联合检测AST/ALT,ALP,γ-GT及AFP灵敏度最高,明显高于AST/ALT,ALP,γ-GT及AFP单项检测(P<0.0 5)。结论 :AST/ALT,γ-GT,ALP及AFP联合检测可提高早期原发性肝癌检出的准确率。 展开更多
关键词 天冬转移/转移比值 碱性磷 r谷转移 甲胎蛋白 原发性肝癌
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