以东方百合“索邦”(L ilium tenu if olium orien ta l‘Sorbonne’)的鳞片、再生形成的小鳞片和叶柄为外植体,进行了愈伤组织诱导、不定芽分化、潮霉素抗性筛选及生根研究。结果表明,3种外植体均可在培养基M S+0.5 m g/L NAA+0.4 m g/...以东方百合“索邦”(L ilium tenu if olium orien ta l‘Sorbonne’)的鳞片、再生形成的小鳞片和叶柄为外植体,进行了愈伤组织诱导、不定芽分化、潮霉素抗性筛选及生根研究。结果表明,3种外植体均可在培养基M S+0.5 m g/L NAA+0.4 m g/L 6-BA+90 g/L Sucrose+4.0 m g/L VB1中形成大量的愈伤组织,愈伤组织分化不定芽的最适培养基为M S+0.5 m g/L 6-BA,筛选的潮霉素质量浓度为20 m g/L,生根培养基为1/2M S+0.2或0.4 m g/L IBA+0.1%活性炭。展开更多
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable c...Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid （HA） on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids ＇Sorbonne＇ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations （_〉2.0 mg/L） within 45 d after transplanting （DAT）. The activities of three major starch synthetic enzymes （including adenosine 5＇-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase） were enhanced dramatically after HA application especially low concentration HA （LHA）, indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg （2.9 times that under the control treatment） and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.展开更多
文摘以东方百合“索邦”(L ilium tenu if olium orien ta l‘Sorbonne’)的鳞片、再生形成的小鳞片和叶柄为外植体,进行了愈伤组织诱导、不定芽分化、潮霉素抗性筛选及生根研究。结果表明,3种外植体均可在培养基M S+0.5 m g/L NAA+0.4 m g/L 6-BA+90 g/L Sucrose+4.0 m g/L VB1中形成大量的愈伤组织,愈伤组织分化不定芽的最适培养基为M S+0.5 m g/L 6-BA,筛选的潮霉素质量浓度为20 m g/L,生根培养基为1/2M S+0.2或0.4 m g/L IBA+0.1%活性炭。
基金Project supported by the National High-Tech R&D Program(863) of China(No.2011AA100208)the Zhejiang Provincial Natural Science Foundation of China(No.LY12C15003)
文摘Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid （HA） on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids ＇Sorbonne＇ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations （_〉2.0 mg/L） within 45 d after transplanting （DAT）. The activities of three major starch synthetic enzymes （including adenosine 5＇-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase） were enhanced dramatically after HA application especially low concentration HA （LHA）, indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg （2.9 times that under the control treatment） and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.
