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用东方螺加工海味即食品的研究 被引量:1
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作者 黄发新 苏滨海 高飞 《肉类研究》 1999年第1期29-32,共4页
介绍以东方螺为原料制造海味即食品工艺和特点,并对制作关键问题进行了探讨,提供有关技术参数。
关键词 海味即食品 东方螺 脱腥
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基于转录组测序方斑东风螺LPS刺激的免疫机制研究
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作者 陈宗发 洪雨洁 +2 位作者 赵明明 王菁 王忠良 《安徽农业科学》 CAS 2023年第4期85-91,共7页
[目的]进一步挖掘方斑东风螺(Babylonia areolata)免疫系统中参与响应脂多糖刺激的重要功能基因与通路。[方法]采用Illumina HiSeqTM 2000高通量测序平台对不同处理条件的方斑东风螺进行测序分析。所得的原始数据经质控、组装后分别比对... [目的]进一步挖掘方斑东风螺(Babylonia areolata)免疫系统中参与响应脂多糖刺激的重要功能基因与通路。[方法]采用Illumina HiSeqTM 2000高通量测序平台对不同处理条件的方斑东风螺进行测序分析。所得的原始数据经质控、组装后分别比对到KOG、Nr、Swiss-Prot和KEGG数据库进行功能注释,筛选差异表达基因并进行聚类分析。[结果]GO功能注释结果显示,多数unigenes被注释到代谢过程(metabolic process)、催化活性(catalytic activity)和细胞过程(cellular process)。KEGG注释结果显示,多数差异表达基因集中于代谢通路(Metabolic pathways)(28.3%)、次生代谢物的生物合成(Biosynthesis of secondary metabolites)(8.69%)、核糖体(Ribosome)(7.47%)和内吞作用(Endocytosis)(5.84%)。对比对照组,试验组中LPS刺激后4 h组具有9096个差异表达基因,8 h组具有10335个差异表达基因,而4 h组和8 h组间则存在8756个差异表达基因,通过KEGG数据库对DEGs进行注释和功能富集,关注到的显著通路包括NOD样受体信号通路、趋化因子信号通路等。[结论]暗示脂多糖调节方斑东风螺的抗病能力通过许多通路,是一个复杂的过程,可为进一步研究方斑东风螺的免疫响应机制研究提供理论数据。 展开更多
关键词 方斑东方螺 转录组 脂多糖 免疫通路
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PCR-DGGE Analysis of Bacterial Communities Structure in Babylonia areolata Culture Systems of The Subtidal Zone and The Pond Mulched Plastic Film and Sand in Bottom
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作者 李淑芳 邱德全 +2 位作者 张继东 杨世平 邱明生 《Agricultural Science & Technology》 CAS 2015年第7期1535-1539,1543,共6页
To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in ... To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in bottom, the bacte- rial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobac- teda Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Garnmaproteobacteria, Alphaproteobacteria, Deltaprotecbacteda, Epsilonproteobacteda, Anaerolineae, Cyanobacteria and Acti- nobacteda. The dominant bacterial communities in the subtidal zone culture system were Gammaprotecbacteda, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteda, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture sys- tems. The results of unweighted pair group method with arithmetic average (UPG- MA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5%. The bacterial communities of seawater samples S3 and S4 in the above culture systems were in clusters, and the similarity of the bacterial communi- ties was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the sub-tidal zone culture systems through changing the pond seawater and monitoring the microbial population. 展开更多
关键词 Bacterial communities structure Denaturing gradient gel electrophoresis (DGGE) Culture system of the sub-tidal zone Culture system of the pond mulched plastic film and sand Babylonia areolata
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