选取在云南高黎贡山赧亢、板场和保山市隆阳区太保公园中录制的东白眉长臂猿3组鸣声,用cool edie pro 2.0软件进行原始数据文件处理,再通过Raven pro 1.4软件进行声谱分析,得到图形化文件.据声谱分析结果,东白眉长臂猿二重唱中,雄性的...选取在云南高黎贡山赧亢、板场和保山市隆阳区太保公园中录制的东白眉长臂猿3组鸣声,用cool edie pro 2.0软件进行原始数据文件处理,再通过Raven pro 1.4软件进行声谱分析,得到图形化文件.据声谱分析结果,东白眉长臂猿二重唱中,雄性的鸣叫序列包括开始音节、重复音节和调节音节;调节音节根据第二个音节的频率变化分为强调音节和弱调音节.雌性的鸣唱序列分为重复音节和激动鸣叫,其激动鸣叫又可分为成功的激动鸣叫和失败的激动鸣叫.比较东白眉长臂猿与东、西黑冠长臂猿的鸣声,发现雌性东白眉长臂猿能够发出黑冠长臂猿雌性个体没有的重复音节.此外,雌性东白眉长臂猿失败的激动鸣叫的出现频率非常高,在一次完整的二重唱中都会存在雌性东白眉长臂猿失败的激动鸣叫,而东黑冠长臂猿的二重唱中没有.展开更多
[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological character...[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological characteristics of the mature seed-like fruits of Salvia shandongensis and its three congeners, S. miltiorrhiza, S. miltiorrhiza f. alba and S. bowleyana, were comparatively observed using both optical microscope and scanning electron microscope. [Result] The results showed that there were distinct differences in the shape, size and exine sculpture of the seed-like fruits of the four Danshen congeners: the seed-like fruits of S. shandongensis that were elliptical and had areolate sculpture with subquadrangular convex meshes as well as densely filamentous transverse lines were easy to be distinguished from those of S. miltiorrhiza Bge., which were broadly elliptical and had reticulate sculpture with subquadrate concave meshes. The seed-like fruits of S. bowleyana were fusiform and their ektexine had reticulate sculpture with polygonal meshes, which were slightly convex at the center, which are obviously different from those of S. shandongensis. [Conclusion] This study first accumulates data of the seed-like fruits of S. shandongensis, which provides important bases for establishing its taxonomical status and studying its germplasm. The morphological characteristics and reticulate sculpture on ektexine of the seed-like fruits S. miltiorrhiza Bge. are consistent with the previous report. The results in this study not only provide references for uncovering the genetic relationships between S. shandongensis and S. miltiorrhiza Bge., as well as the interspecific classification and identification. In addition, this study also provides new scientific proofs for the identification of Danshen species and their introduction and cultivation, genetics and breeding.展开更多
Larch (Larix olgensis), Manchurian ash (Fraxinus mandshurica), Korean pine (Pinus koraiensis) and White birch (Betula platyphylla) are the major planting species in northeast China. The samples of forest litters were ...Larch (Larix olgensis), Manchurian ash (Fraxinus mandshurica), Korean pine (Pinus koraiensis) and White birch (Betula platyphylla) are the major planting species in northeast China. The samples of forest litters were collected from the stands of the above 4 species in Laoyeling and Jianlagou experiment stations of Maorshan Exp. Forest Farm (45?2-45?0N, 127?0-127?8E), Northeast Forestry University, in early October 2002. Quantitative analysis and qualitative analysis were carried out on the organic acids existing in freshly fallen litters (L layer) and hemi-decomposed litters (F layer) of the four forest species by using Gas Chromatogram system. A wide variety of organic acids were identified, including oxalic, malonic, fumaric, succinic, maleic, malic, citric, C16:0, C18:0, C18:1, C18:2, C18:3 and C20:0 acids. In respect of L litters of all samples, the oxalic acid content (over 30 mg/g) was the highest of the seven low-molecular-weight organic acids identified, while the content of oleic or linoleic (above 40 mg/g) was found to be highest among the six high aliphatic acids identified. As to F litters, oxalic acid content was also the highest, followed by linoleic and oleic. For the same tree species or the same forest, the kinds and contents of organic acids in L litters were more abundant than that in F litters.展开更多
The spatial distribution and population dynamics of Quercus liaotungensis Koidz., Fraxinus rhynchophylla Hance and Acer mono Maxim. were studied in three warm temperate deciduous forests in Dongling Mountain, Beijing....The spatial distribution and population dynamics of Quercus liaotungensis Koidz., Fraxinus rhynchophylla Hance and Acer mono Maxim. were studied in three warm temperate deciduous forests in Dongling Mountain, Beijing. Clumped distributions appeared in most seedlings (≤0.4 m), short saplings (0.4-1 m) and tall saplings (1-2 m). Random distributions appeared in adults of Q. liaotungensis in Plot 1, F. rhynchophylla in Plot 3. The LSD method was used to discern the differences of different aged population. The class distribution of Q. liaotungensis was nearly a reverse-J shape for Plot 1 and Plot 3, suggesting that the population regenerates continuously. The age-structures of F. rhynchophylla and A. mono in Plot 1 and the test results indicated that they would increase continuously. In Plot 2, the class distribution and the test results showed that all populations would not regenerate continuously.展开更多
[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, disso...[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K(2n) =3x=24m(2SAT) + 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.展开更多
The community characteristics of natural secondary forests on the north slope of Changbai Mountain after selective cutting were investigated, and the dynamics of arborous species diversity during the restoration perio...The community characteristics of natural secondary forests on the north slope of Changbai Mountain after selective cutting were investigated, and the dynamics of arborous species diversity during the restoration period of 28 years were studied. The results showed that the arborous species richness (S) had little change and kept the range of 18-22 all along, the Simpson index (D) of the secondary layer and regeneration layer and whole stand had similar trends of change, but that of the canopy layer descended slowly in initial 15 years and had little change later, and the change of diversity index was not obvious and the Shannon-Wiener index (H? fluctuated in a very small scopes (H±10%).展开更多
Environmental factors affect animal behavior in a variety of ways. Compared to animals in the wild, captive animals are kept in narrow enclosures, suffer higher densities and are more intensely affected by human activ...Environmental factors affect animal behavior in a variety of ways. Compared to animals in the wild, captive animals are kept in narrow enclosures, suffer higher densities and are more intensely affected by human activities. Therefore, behavioral elasticity is likely modified to some extent in captive animals, which may negatively affect the possibility of successful release of endangered species in the wild. In our study we investigated the behaviors of the Oriental White Stork (Ciconia boyciana) in a semi-natural enclosure from 15 October 2005 to 8 September 2006 in Hefei Wildlife Park, China. The behavioral data were collected by instantaneous and scan sampling methods, and we analyzed the relationships between environmental factors and their behaviors. The amounts of time spent on maintenance behavior (foraging and moving) were at similar levels for birds reproducing multibroods (Group 1), birds reproducing one brood (Group 2) and birds without reproduction (Group 3). On the other hand, the amount of time spent on elastic behavior (defensive, breeding and preening) for Group 3 was lower than that for Groups 1 and 2. The behavioral pattern of the storks exhibited marked seasonal changes, which may be a function of ambient temperature, human disturbanc, and the length of daylight, but not of wind intensity. Our results provide basic data for future conser- vation measures of this endangered species.展开更多
The Oriental White Stork (Ciconia boyciana) is a large and endangered waterbird in East Asia.Research on conservation genetics of this species is urgently needed.In this study,microsatellite marking technology was use...The Oriental White Stork (Ciconia boyciana) is a large and endangered waterbird in East Asia.Research on conservation genetics of this species is urgently needed.In this study,microsatellite marking technology was used for screening and analysis of genetic diversity of microsatellite markers in Oriental White Storks.A total of 36 pairs of microsatellite primers were used,of which 7 pairs came from Ciconia ciconia,12 pairs from Nipponia nippon and 17 pairs from Ardea herodias.Microsatellite loci were screened from 23 individuals of the Oriental White Stork and 11 microsatellite loci were found with high polymorphism.The number of population alleles ranged from 3 to 11,averaging 7.09.The average expected heterozygosity (H e) was 0.7816 and the average polymorphism information content (PIC) 0.7172,suggesting a relatively high genetic diversity in the population.展开更多
Although chub mackerel (Scomberjaponicus) is a primary pelagic fish species, we have only limited knowledge on its key life history processes. The present work studied the age and growth of chub mackerel in the East...Although chub mackerel (Scomberjaponicus) is a primary pelagic fish species, we have only limited knowledge on its key life history processes. The present work studied the age and growth of chub mackerel in the East China and Yellow Seas. Age was determined by interpreting and counting growth rings on the sagitta otoliths of 252 adult fish caught by the Chinese commercial purse seine fleet during the period from November 2006 to January 2007 and 150 juveniles from bottom trawl surveys on the spawning ground in May 2006. The difference between the assumed birth date of 1st April and date of capture was used to adjust the age determined from counting the number of complete translucent rings. The parameters of three commonly used growth models, the von Bertalanffy, Logistic and Gompertz models, were estimated using the maximum likelihood method. Based on the Akaike Information Criterion (AIC), the yon Bertalanffy growth model was found to be the most appropriate model. The size-at-age and size-at-maturity values were also found to decrease greatly compared with the results achieved in the 1950s, which was caused by heavy exploitation over the last few decades.展开更多
To improve the burial flux calculations of bioavailable phosphorus (P) and study opal-associated P (Opal-P) in the East China Sea (ECS), surface and core sediments were collected in the Changjiang Estuary (CE)...To improve the burial flux calculations of bioavailable phosphorus (P) and study opal-associated P (Opal-P) in the East China Sea (ECS), surface and core sediments were collected in the Changjiang Estuary (CE) and the south of the Cheju Island. In this study, sedimentary P was operationally divided into seven different forms using modified sedimentary extraction (SEDEX) technique: LSor-P (exchangeable or loosely sorbed P), Fe-P (easily reducible or reactive ferric Fe-bound P), CFA-P (authigenic carbonate fluorapatite and biogenic apatite and CaCO3-bound P), Detr-P (detrital apatite), Org-P (organic P), Opal-P and Ref-P (refractory P). The data revealed that the concentrations of the seven different P forms rank as Detr-P 〉 CFA-P 〉 Org-P 〉 Ref-P 〉 Opal-P 〉 Fe-P 〉 LSor-P in surface sediments and CFA-P 〉 Detr-P 〉 Org-P 〉 Ref-P 〉 Fe-P 〉 Opal-P 〉 LSor-P in core sediments. The distributions of the total phosphorus (TP), TIP, CFA-P, Detr-P are similar and decrease from the CE to the south of the Cheju Island. Meanwhile, Org-P and Opal-P exhibit different distribution trends; this may be affected by the grain size and TOM. The concentrations of potentially bioavailable P are 9.6-13.0 μmol g^-1 and 10.0-13.6 μmol g^-1, representing 61%-70% and 41%?64% of the TP in surface and core sediments, respectively. The concentrations of Opal-P are 0.6-2.3 μmol g^-1 and 0.6-1.4 μmol g^-1 in surface and core sediments, ac-counting for 5.3%?19.8% and 4.2%?10.6% of bioavailable P, respectively. The total burial fluxes of Opal-P and bioavailable P are 1.4×10^9 mol y^-1 and 1.1×10^10 mol yr^-1 in the ECS, respectively. Opal-P represents about 12.7% of potentially bioavailable P, which should be recognized when studying P cycling in marine ecosystems.展开更多
文摘选取在云南高黎贡山赧亢、板场和保山市隆阳区太保公园中录制的东白眉长臂猿3组鸣声,用cool edie pro 2.0软件进行原始数据文件处理,再通过Raven pro 1.4软件进行声谱分析,得到图形化文件.据声谱分析结果,东白眉长臂猿二重唱中,雄性的鸣叫序列包括开始音节、重复音节和调节音节;调节音节根据第二个音节的频率变化分为强调音节和弱调音节.雌性的鸣唱序列分为重复音节和激动鸣叫,其激动鸣叫又可分为成功的激动鸣叫和失败的激动鸣叫.比较东白眉长臂猿与东、西黑冠长臂猿的鸣声,发现雌性东白眉长臂猿能够发出黑冠长臂猿雌性个体没有的重复音节.此外,雌性东白眉长臂猿失败的激动鸣叫的出现频率非常高,在一次完整的二重唱中都会存在雌性东白眉长臂猿失败的激动鸣叫,而东黑冠长臂猿的二重唱中没有.
基金Supported by the Scientific and Technological Development Project of Shandong Province(2008GG2NS02022)the Special Fund for Agricultural Fine Seed Project in Shandong Province(2009LZ01-03)~~
文摘[Objective] This study aimed to compare and analyse the morphological characteristics of the seed-like fruits between Salvia shandongensis J. X. Li et F. Q. Zhou and its relatives. [Method] The morphological characteristics of the mature seed-like fruits of Salvia shandongensis and its three congeners, S. miltiorrhiza, S. miltiorrhiza f. alba and S. bowleyana, were comparatively observed using both optical microscope and scanning electron microscope. [Result] The results showed that there were distinct differences in the shape, size and exine sculpture of the seed-like fruits of the four Danshen congeners: the seed-like fruits of S. shandongensis that were elliptical and had areolate sculpture with subquadrangular convex meshes as well as densely filamentous transverse lines were easy to be distinguished from those of S. miltiorrhiza Bge., which were broadly elliptical and had reticulate sculpture with subquadrate concave meshes. The seed-like fruits of S. bowleyana were fusiform and their ektexine had reticulate sculpture with polygonal meshes, which were slightly convex at the center, which are obviously different from those of S. shandongensis. [Conclusion] This study first accumulates data of the seed-like fruits of S. shandongensis, which provides important bases for establishing its taxonomical status and studying its germplasm. The morphological characteristics and reticulate sculpture on ektexine of the seed-like fruits S. miltiorrhiza Bge. are consistent with the previous report. The results in this study not only provide references for uncovering the genetic relationships between S. shandongensis and S. miltiorrhiza Bge., as well as the interspecific classification and identification. In addition, this study also provides new scientific proofs for the identification of Danshen species and their introduction and cultivation, genetics and breeding.
基金This paper is supported by National Natural Science Foundation of China (30170768)
文摘Larch (Larix olgensis), Manchurian ash (Fraxinus mandshurica), Korean pine (Pinus koraiensis) and White birch (Betula platyphylla) are the major planting species in northeast China. The samples of forest litters were collected from the stands of the above 4 species in Laoyeling and Jianlagou experiment stations of Maorshan Exp. Forest Farm (45?2-45?0N, 127?0-127?8E), Northeast Forestry University, in early October 2002. Quantitative analysis and qualitative analysis were carried out on the organic acids existing in freshly fallen litters (L layer) and hemi-decomposed litters (F layer) of the four forest species by using Gas Chromatogram system. A wide variety of organic acids were identified, including oxalic, malonic, fumaric, succinic, maleic, malic, citric, C16:0, C18:0, C18:1, C18:2, C18:3 and C20:0 acids. In respect of L litters of all samples, the oxalic acid content (over 30 mg/g) was the highest of the seven low-molecular-weight organic acids identified, while the content of oleic or linoleic (above 40 mg/g) was found to be highest among the six high aliphatic acids identified. As to F litters, oxalic acid content was also the highest, followed by linoleic and oleic. For the same tree species or the same forest, the kinds and contents of organic acids in L litters were more abundant than that in F litters.
文摘The spatial distribution and population dynamics of Quercus liaotungensis Koidz., Fraxinus rhynchophylla Hance and Acer mono Maxim. were studied in three warm temperate deciduous forests in Dongling Mountain, Beijing. Clumped distributions appeared in most seedlings (≤0.4 m), short saplings (0.4-1 m) and tall saplings (1-2 m). Random distributions appeared in adults of Q. liaotungensis in Plot 1, F. rhynchophylla in Plot 3. The LSD method was used to discern the differences of different aged population. The class distribution of Q. liaotungensis was nearly a reverse-J shape for Plot 1 and Plot 3, suggesting that the population regenerates continuously. The age-structures of F. rhynchophylla and A. mono in Plot 1 and the test results indicated that they would increase continuously. In Plot 2, the class distribution and the test results showed that all populations would not regenerate continuously.
基金Supported by the Program from Ludong University(043312)~~
文摘[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K(2n) =3x=24m(2SAT) + 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.
基金This research was supported by Institute of Shenyang Applied Ecology CAS (SCXMS0101),National Key Technologies R&D Program (NKTRDP. 2002BA516A20) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education
文摘The community characteristics of natural secondary forests on the north slope of Changbai Mountain after selective cutting were investigated, and the dynamics of arborous species diversity during the restoration period of 28 years were studied. The results showed that the arborous species richness (S) had little change and kept the range of 18-22 all along, the Simpson index (D) of the secondary layer and regeneration layer and whole stand had similar trends of change, but that of the canopy layer descended slowly in initial 15 years and had little change later, and the change of diversity index was not obvious and the Shannon-Wiener index (H? fluctuated in a very small scopes (H±10%).
基金supported by the National Natural Science Foundation of China (Grant No. 3087317, 30470257)the Anhui Academic and Technical Leaders Fund
文摘Environmental factors affect animal behavior in a variety of ways. Compared to animals in the wild, captive animals are kept in narrow enclosures, suffer higher densities and are more intensely affected by human activities. Therefore, behavioral elasticity is likely modified to some extent in captive animals, which may negatively affect the possibility of successful release of endangered species in the wild. In our study we investigated the behaviors of the Oriental White Stork (Ciconia boyciana) in a semi-natural enclosure from 15 October 2005 to 8 September 2006 in Hefei Wildlife Park, China. The behavioral data were collected by instantaneous and scan sampling methods, and we analyzed the relationships between environmental factors and their behaviors. The amounts of time spent on maintenance behavior (foraging and moving) were at similar levels for birds reproducing multibroods (Group 1), birds reproducing one brood (Group 2) and birds without reproduction (Group 3). On the other hand, the amount of time spent on elastic behavior (defensive, breeding and preening) for Group 3 was lower than that for Groups 1 and 2. The behavioral pattern of the storks exhibited marked seasonal changes, which may be a function of ambient temperature, human disturbanc, and the length of daylight, but not of wind intensity. Our results provide basic data for future conser- vation measures of this endangered species.
基金supported by the National Natural Science Foundation of China(Grant No.3087317)the Anhui Academic and Technical Reserve Candidate Leaders Fund
文摘The Oriental White Stork (Ciconia boyciana) is a large and endangered waterbird in East Asia.Research on conservation genetics of this species is urgently needed.In this study,microsatellite marking technology was used for screening and analysis of genetic diversity of microsatellite markers in Oriental White Storks.A total of 36 pairs of microsatellite primers were used,of which 7 pairs came from Ciconia ciconia,12 pairs from Nipponia nippon and 17 pairs from Ardea herodias.Microsatellite loci were screened from 23 individuals of the Oriental White Stork and 11 microsatellite loci were found with high polymorphism.The number of population alleles ranged from 3 to 11,averaging 7.09.The average expected heterozygosity (H e) was 0.7816 and the average polymorphism information content (PIC) 0.7172,suggesting a relatively high genetic diversity in the population.
基金the Program for New Century Excellent Talents in University (NCET-06-0437)Shanghai Leading Academic Discipline Project (No T1101)
文摘Although chub mackerel (Scomberjaponicus) is a primary pelagic fish species, we have only limited knowledge on its key life history processes. The present work studied the age and growth of chub mackerel in the East China and Yellow Seas. Age was determined by interpreting and counting growth rings on the sagitta otoliths of 252 adult fish caught by the Chinese commercial purse seine fleet during the period from November 2006 to January 2007 and 150 juveniles from bottom trawl surveys on the spawning ground in May 2006. The difference between the assumed birth date of 1st April and date of capture was used to adjust the age determined from counting the number of complete translucent rings. The parameters of three commonly used growth models, the von Bertalanffy, Logistic and Gompertz models, were estimated using the maximum likelihood method. Based on the Akaike Information Criterion (AIC), the yon Bertalanffy growth model was found to be the most appropriate model. The size-at-age and size-at-maturity values were also found to decrease greatly compared with the results achieved in the 1950s, which was caused by heavy exploitation over the last few decades.
基金financially supported by the National Natural Science Foundation of China (Nos. 41530965, 41276071, 41003052)the Fundamental Research Funds for the Central Universities (No. 201564008)
文摘To improve the burial flux calculations of bioavailable phosphorus (P) and study opal-associated P (Opal-P) in the East China Sea (ECS), surface and core sediments were collected in the Changjiang Estuary (CE) and the south of the Cheju Island. In this study, sedimentary P was operationally divided into seven different forms using modified sedimentary extraction (SEDEX) technique: LSor-P (exchangeable or loosely sorbed P), Fe-P (easily reducible or reactive ferric Fe-bound P), CFA-P (authigenic carbonate fluorapatite and biogenic apatite and CaCO3-bound P), Detr-P (detrital apatite), Org-P (organic P), Opal-P and Ref-P (refractory P). The data revealed that the concentrations of the seven different P forms rank as Detr-P 〉 CFA-P 〉 Org-P 〉 Ref-P 〉 Opal-P 〉 Fe-P 〉 LSor-P in surface sediments and CFA-P 〉 Detr-P 〉 Org-P 〉 Ref-P 〉 Fe-P 〉 Opal-P 〉 LSor-P in core sediments. The distributions of the total phosphorus (TP), TIP, CFA-P, Detr-P are similar and decrease from the CE to the south of the Cheju Island. Meanwhile, Org-P and Opal-P exhibit different distribution trends; this may be affected by the grain size and TOM. The concentrations of potentially bioavailable P are 9.6-13.0 μmol g^-1 and 10.0-13.6 μmol g^-1, representing 61%-70% and 41%?64% of the TP in surface and core sediments, respectively. The concentrations of Opal-P are 0.6-2.3 μmol g^-1 and 0.6-1.4 μmol g^-1 in surface and core sediments, ac-counting for 5.3%?19.8% and 4.2%?10.6% of bioavailable P, respectively. The total burial fluxes of Opal-P and bioavailable P are 1.4×10^9 mol y^-1 and 1.1×10^10 mol yr^-1 in the ECS, respectively. Opal-P represents about 12.7% of potentially bioavailable P, which should be recognized when studying P cycling in marine ecosystems.