为建立产琥珀酸丝状杆菌定性定量研究方法,实现后期该菌种用于纤维素生物可再生能源等方面应用研究。本试验提取15份瘤胃细菌脱氧核糖核酸(Deoxyribonucleic acid,DNA),利用SYBR Premix Ex Taq荧光染料嵌合法和人工合成基因技术,采用实...为建立产琥珀酸丝状杆菌定性定量研究方法,实现后期该菌种用于纤维素生物可再生能源等方面应用研究。本试验提取15份瘤胃细菌脱氧核糖核酸(Deoxyribonucleic acid,DNA),利用SYBR Premix Ex Taq荧光染料嵌合法和人工合成基因技术,采用实时荧光定量PCR方法检测样品中产琥珀酸丝状杆菌数量;结果:所构建的质粒DNA标准曲线线性关系良好,R2=1,所有样品溶解曲线均为单峰,扩增曲线呈S型,扩增效率为93.9%。表明应用构建实时荧光定量PCR法可对实际样品中产琥珀酸丝状杆菌进行定性定量研究。展开更多
In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous f...In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous form identified as Chroococcus sp. and Lyngbya sp.. After identification of genera their optimum growth condition studied by using the effect of temperature and pH to their dry weight. In the result, the optimum temperature and pH for both filamentous cyanobacteria were 25 ℃ and pH 7.5. Both cyanobacterial strains were extracted with ethanol, methanol and diethyl ether at various concentrations （0.2 g/mL, 0.1 g/mL, 0.005 g/mL） which exhibited the antibacterial activities against Staphylococcus aureus, E. coli and B. subtilus. Inhibition activities of the two cyanobacterial extracts were more effective at high concentration against the tested pathogens at the low concentration, especially those ofLyngbya sp. The higher inhibition zone showed with extract by ethanol.展开更多
文摘为建立产琥珀酸丝状杆菌定性定量研究方法,实现后期该菌种用于纤维素生物可再生能源等方面应用研究。本试验提取15份瘤胃细菌脱氧核糖核酸(Deoxyribonucleic acid,DNA),利用SYBR Premix Ex Taq荧光染料嵌合法和人工合成基因技术,采用实时荧光定量PCR方法检测样品中产琥珀酸丝状杆菌数量;结果:所构建的质粒DNA标准曲线线性关系良好,R2=1,所有样品溶解曲线均为单峰,扩增曲线呈S型,扩增效率为93.9%。表明应用构建实时荧光定量PCR法可对实际样品中产琥珀酸丝状杆菌进行定性定量研究。
文摘In the study cyanobacterial strains were isolated from different sites of Hizoop rivers, Koya-Iraq and identified according to their morphological characters by using microscope, two genera which were in filamentous form identified as Chroococcus sp. and Lyngbya sp.. After identification of genera their optimum growth condition studied by using the effect of temperature and pH to their dry weight. In the result, the optimum temperature and pH for both filamentous cyanobacteria were 25 ℃ and pH 7.5. Both cyanobacterial strains were extracted with ethanol, methanol and diethyl ether at various concentrations （0.2 g/mL, 0.1 g/mL, 0.005 g/mL） which exhibited the antibacterial activities against Staphylococcus aureus, E. coli and B. subtilus. Inhibition activities of the two cyanobacterial extracts were more effective at high concentration against the tested pathogens at the low concentration, especially those ofLyngbya sp. The higher inhibition zone showed with extract by ethanol.
