药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征....药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征.使用荧光漂白恢复法(FRAP,fluorescence recovery after photobleaching)观察荧光素FITC标记的牛血清白蛋白(BSA)在聚丙烯酰胺-丙烯酸中的扩散行为,并以激光共聚焦显微镜进行实时成像.实验表明,FITC-BSA在不同单体配比的共聚物中的扩散系数是不同的.通过调节聚合物中单体的配比能够达到控制蛋白释放速率的作用,从而为调控蛋白和多肽类药物的缓控释放提供了可能性.展开更多
The adsorption of serum proteins on polymer microspheres is very important for their application in many fields.Various amounts of sulfonate groups were introduced to PMMA microspheres, and the adsorption behaviors of...The adsorption of serum proteins on polymer microspheres is very important for their application in many fields.Various amounts of sulfonate groups were introduced to PMMA microspheres, and the adsorption behaviors of BSA on them was studied. The apparent rate constants of various microspheres increased with the increase amount of sulfonate groups on the surface of microspheres. Adsorption isotherm curves were fitted to the Langmuir-Freundlich model. With the increase of sulfonate groups on the surface of microspheres, c-m values increased, however, K showed an opposite tendency to c-m. It is indicated that the electrostatic interaction plays a dominant role in the adsorption process, and adsorption based on electrostatic interaction is higher than that due to hydrophobic interaction, but the adsorption due to electrostatic interaction has a lower rate than that of the latter.展开更多
文摘药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征.使用荧光漂白恢复法(FRAP,fluorescence recovery after photobleaching)观察荧光素FITC标记的牛血清白蛋白(BSA)在聚丙烯酰胺-丙烯酸中的扩散行为,并以激光共聚焦显微镜进行实时成像.实验表明,FITC-BSA在不同单体配比的共聚物中的扩散系数是不同的.通过调节聚合物中单体的配比能够达到控制蛋白释放速率的作用,从而为调控蛋白和多肽类药物的缓控释放提供了可能性.
文摘The adsorption of serum proteins on polymer microspheres is very important for their application in many fields.Various amounts of sulfonate groups were introduced to PMMA microspheres, and the adsorption behaviors of BSA on them was studied. The apparent rate constants of various microspheres increased with the increase amount of sulfonate groups on the surface of microspheres. Adsorption isotherm curves were fitted to the Langmuir-Freundlich model. With the increase of sulfonate groups on the surface of microspheres, c-m values increased, however, K showed an opposite tendency to c-m. It is indicated that the electrostatic interaction plays a dominant role in the adsorption process, and adsorption based on electrostatic interaction is higher than that due to hydrophobic interaction, but the adsorption due to electrostatic interaction has a lower rate than that of the latter.
