高性能柔性OFET器件最新进展研究

有机场效应晶体管,因其质轻价廉且与柔性衬底兼容等优点,广泛应用于传感器阵列,平板显示器和射频识别等许多领域。为了获得迁移率高、空气稳定性好、机械柔韧性佳的高性能柔性OFET,本文以对器件性能影响较为显著的关键因素为出发点,阐述了柔性OFET器件衬底材料、绝缘层材料、电极材料及制备工艺的最新进展情况。结果表明,全有机柔性OFET器件具备低压操作,良好电荷捕获能力,弯曲时器件特性稳定等优点,适合大规模商业化使用。

Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination

The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL 1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.

Structure and Property of Silk Fibroin / Cellulose Blend Film

Silk fibroin/cellulose blend films were prepared using N-methylmorpholine -N-oxide (NMMO) as solvent. The effects of different proportions and solid contents on properties of blend films were discussed. The mechanical properties showed that the blend films had preferable moisture permeability and a high strength. The structures of the blend films were investigated by infrared spectrum and X-ray diffraction. The results indicated the occurrence of hydrogen bonds between hydroxyl groups of cellulose and amido groups of fibroin.

Study on Electrospinning Silk Fibroin Solution

A new method of preparing silk fibroin （SF） solution used in the decterospinning was introduced in this paper. According to the method, SF was dissolved in the LiBr/CH2O2 solution directly at room temperature. The method was compared with the traditional method--SF was dissolved in CaCl2 ternary solution. The structure of SF films and the morphology of SF nanofibers were examined by attenuated total reflectance fourier transform intrared （ATR- FTIR） spectroscopy, Scanning electron microscope （SEM） and optical polarizing microscope. The result of this study shows that the new method is a faster, more convenient and high cfficieat way to get the SF solution and the characteristics of SF fiber made by the new metbod is much better.

Variation and Characterization Analysis of Partial Fragment of Fibroin Gene From Silkworm, Antheraea pernyi

A 1.4Kb DNA fragment containing 3’ flanking sequence of fibroin gene of silkworm, Antheraea pernyi, was obtained from the silk gland’s mRNA of 5 th larva. Analysis of this sequence with another A.pernyi fibroin protein (accession No. D83241) revealed that it consists of a completely open reading frame (ORF), which includes 14 polyalanine-containing units (motifs) and 100bp 3’-UTR. The sequence of the predicted amino acid reveals the highest level of overall identity (90%) with D83241. It was found that it loses a repeat region at the upstream of TAA codon and some mutations. A putative polyadenylation signal AATAAA tail was found in position 1300, which follows the termination codon.

Surface Modification of Anionic Polyurethane with Silk Fibroin Peptide and Its Effects on the Culture of Fibroblasts

The surface modification of the anionic polyurethane(APU)film was carried out by immersing it in silk fibroin peptide(SFP)solution for 12 h and then treating with low temperature plasma glow discharge.The physical properties and moisture permeability of modified films were examined.The results showed that SFP-modified APU films had better moisture permeability than oleophilic polyurethane,as well as modified APU films kept good flexibility.Modified APU films could overcome rigid and brittle weaks of silk fibroin films.The morphology of SFP on the APU film was corpuscular aggregations.The water-contact angle measurement indicated that the change of hydrophilicity and the element chemical analysis suggested that the SFP-modified film surface was enriched with nitrogen atoms.The biocompatibility of APU films may be improved due to the change of surface components.Cell viability and proliferation of rat embryo dermal fibroblasts seeded on control films,APU films and SFP-modified APU films were evaluated by MTT assay and viable cell counts,respectively.The results indicated that the APU film modified by SFP protein showed the proliferation of fibroblasts on the film,and that the compound interface had good stability in the air.Results also showed that presoaking treatment for APU films was effective to accomplish the goal of surface modification.

The Mulberry Silkworm--A New Source of Bioactive Proteins

Beyond the commonly known proteins built silk fibres--fibroin and sericin, there are almost 300 bioactive proteins in the silkworm haemolymph. The aim of this work was to present bioactive compounds obtained from the silk fibre and isolated from the body of this insect, which may be used in medical and pharmacological applications. The most important are bioactive proteins. However, the juvenile stages of mulberry silkworm possess other very valuable active substances.

Characterization of kinesin-like proteins in silkworm posterior silkgland cells

Kinesins are microtubule-based motors involved in various intracellular transports. Neurons, flagellated cells, and pigment cells have been traditionally used as model systems to study the cellular functions of kinesins. Here, we report silkworm posterior silkgland （PSG）, specialized cells with an extensive endomembrane system for intracellular transport and efficient secretion of fibroin, as a novel model for kinesin study. To investigate kinesindriven intracellular transport in PSG cells, we cloned five silkworm kinesin-like proteins （KLPs）, BmKinesin-1, BmKinesin-6, BmKinesin-7, BmKinesin-13, and BmKinesin-14A. We determined their expression patterns by relative real-time PCR and western blotting. Immunofluorescence microscopy verified their colocalization with microtubules. By combining pull-down assays, LC-MS/MS, and western blotting analysis, we identified many potential cargoes of BmKinesin-1 in PSG, including fibroin-containing granules and exuperantia-associated ribonucleoprotein （RNP） complexes. Moreover, BmKinesin-13 overexpression disrupted the microtubule network in BmN cells, which is consistent with a role of Kinesin-13 in regulating microtubule dynamics in other organisms. On the basis of these results, we concluded that PSG might have advantages in elucidating mechanisms of intracellular transport in secretory tissues and could serve as a potential model for kinesin studies.