植物人工免疫的抗病性可以遗传Ι.给大白菜免疫TyMV病毒病

人工免疫的抗病性能够遗传,一直是农医科学工作者的一个梦!作者经过32年研究,发现了生物突变和进化的机制,是生物体受不利于生存因素刺激产生的应对信息,恰遇其精卵两性细胞融合时,便进入其中,作为下代抵抗同样因素的"记忆",从而建立了圆梦的理论根据。在实验中,第一次在大白菜的二牛心品种上,用TyMV病毒免疫病毒病,使一个常遭毁灭性危害的品种,在病毒病大流行年也不发病,并稳定遗传,在生产中发挥着重要作用。第二次实验在大白菜724品系上做TuMV免疫处理后,连续鉴定5代病情指数都不超过10.49,把一个原来近于高感的品系免疫到了高抗标准,就认证了人工免疫的抗病性可以稳定遗传。同时发现了两次免疫效果差异的原因,十分珍贵。联系在西瓜上免疫枯萎病的病情指数由30.17降低到稳定的1.2的例子,就自然认证了人工免疫的抗病性是可以遗传的,对生物突变与进化的机制的论证是正确的,可借鉴面广,意义重大。

发现生物突变和进化的机制

发现了只有正在融合的两性细胞才是执行突变的场所,只有不利于生存的因素发出的信息,才能引起生物体产生应对信息,这正是生物为不断完善自身生存能力需要的有利信息。短促的两性细胞融合时间恰与应对信息同时发生,才能给子代留下一份应对信息的记忆,在一定条件下稳定遗传。几个免疫植物病害的实验都证实了这项论证正确,突破了人工免疫的抗性不能遗传的世纪难题,应用前途广阔,易普及。

Comparative investigation on spindle behavior and MPF activity changes during oocyte maturation between gynogenetic and amphimictic crucian carp

The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimictic colored crucian carp. MPF activity was measured by using histone H1 as phosphorylation substrate. There were two similar oscillatory MPF kinase activity changes during oocyte maturation in two kinds of fishes with different reproductive modes, but there existed some subtle difference between them. The subtle difference was that the first peak of MPF kinase activity was kept to a longerlasting time in the gynogenetic silver crucian carp than in the amphimictic colored crucian carp. It was suggested that the difference may be related to the spindle behavior changes, such as tripolar spindle formation and spindle rearrangement in the gynogenetic crucian carp.