Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in ha...Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8 d of hatching. The thymus appeared after 15 d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40 d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.展开更多
To investigate the expression characteristics of basic fibroblast growth factor （ bFGF ） and its receptors, fig （ FGFR1 ） and bek （ FGFR2 ）, in fetal skin at different gestational ages underlying the relevance o...To investigate the expression characteristics of basic fibroblast growth factor （ bFGF ） and its receptors, fig （ FGFR1 ） and bek （ FGFR2 ）, in fetal skin at different gestational ages underlying the relevance of these 3 proteins to skin development and the mechanisms underlying the phenotypic transition from scarless to scar- forming healing. Methods ： Eighteen specimens of fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages of 13-32 weeks. Gene expression of bFGF, bek and fig was examined with reverse transcription-polymerase chain reaction （ RT-PCR ）. The dynamic expression and distribution of these 3 proteins were detected with streptavidin peroxidase （ SP ） immunohistochemical staining method. Results： In the early gestational fetal skin, genes of bFGF and fig were strongly expressed and more protein contents of these 2 proteins were found as compared with the genes at late gestation fetal skin （2.446 ± 0. 116 and 2.066±0.152 versus 2. 157± 0.101 and 1.818± 0.086, respectively, P 〈 0.05 ）. On the contrary, the levels of gene expression and protein content of bek were not differently expressed in the early gestational fetal skin versus the late ones. Protein particles of bFGF were mainly distributed in the epidermal cells and some fibroblasts. Bek was mainly located in the cell membrane and cytoplasm of epidermal cells while fig protein was principally located in the epidermal cells, endothelial cells and some fibroblasts. Conclusions： The endogenous bFGF and their refeptors might be involved in the cutaneous development at fetal stage. The differently expressing levels of bFGF and fig during gestation may be related to scarless or scarforming repair during gestation.展开更多
基金supported by the National Natural Science of China(No.30000129)Ministry of Science and Technology of China(G1999012005).
文摘Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8 d of hatching. The thymus appeared after 15 d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40 d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.
基金This work was supported by the National Basic Science andDevelopment Program (973 Program, No.G1999054204)andthe National Natural Science Foundation of China ( No.30230370 and No. 30400172)
文摘To investigate the expression characteristics of basic fibroblast growth factor （ bFGF ） and its receptors, fig （ FGFR1 ） and bek （ FGFR2 ）, in fetal skin at different gestational ages underlying the relevance of these 3 proteins to skin development and the mechanisms underlying the phenotypic transition from scarless to scar- forming healing. Methods ： Eighteen specimens of fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages of 13-32 weeks. Gene expression of bFGF, bek and fig was examined with reverse transcription-polymerase chain reaction （ RT-PCR ）. The dynamic expression and distribution of these 3 proteins were detected with streptavidin peroxidase （ SP ） immunohistochemical staining method. Results： In the early gestational fetal skin, genes of bFGF and fig were strongly expressed and more protein contents of these 2 proteins were found as compared with the genes at late gestation fetal skin （2.446 ± 0. 116 and 2.066±0.152 versus 2. 157± 0.101 and 1.818± 0.086, respectively, P 〈 0.05 ）. On the contrary, the levels of gene expression and protein content of bek were not differently expressed in the early gestational fetal skin versus the late ones. Protein particles of bFGF were mainly distributed in the epidermal cells and some fibroblasts. Bek was mainly located in the cell membrane and cytoplasm of epidermal cells while fig protein was principally located in the epidermal cells, endothelial cells and some fibroblasts. Conclusions： The endogenous bFGF and their refeptors might be involved in the cutaneous development at fetal stage. The differently expressing levels of bFGF and fig during gestation may be related to scarless or scarforming repair during gestation.
