Previous research on gene expression analysis and association tests have suggested that RELN is a risk gene for schizophrenia in world populations.Based on the reported down-regulation of RELN in schizophrenia patient...Previous research on gene expression analysis and association tests have suggested that RELN is a risk gene for schizophrenia in world populations.Based on the reported down-regulation of RELN in schizophrenia patients compared with normal subjects,we speculated that variants in the RELN promoter region may confer risk for schizophrenia.In this study,we investigated the associations of three SNPs in the promoter region of RELN with schizophrenia in a case-control sample from southwestern China（940 cases and 13 69 controls）.The results suggested that none of the SNPs showed significant associations in our sample,indicating the risk variants for schizophrenia in RELN may not be located in the promoter region.We also performed meta-analysis by combining our data with previously reported data on the Chinese population with a total sample size of 2 843 individuals,and the result remained nonsignificant.Collectively,our results suggested variants in the RELN promoter may not harbor risk SNPs associated with schizophrenia in the Chinese population.展开更多
Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mit...Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.展开更多
Chinese shrimp （Fenneropenaeus chinensis） is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to i...Chinese shrimp （Fenneropenaeus chinensis） is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization （FISH） was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene （rDNA） of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of homologous chromosomes in F chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in E chinensis. The successful application ofFISH in E chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes ofpenaeid shrimps.展开更多
The shell model calculations in the sdgh major shell for the neutron-deficient ^106,107,108,109Sn isotopes have been carried out by using CD-Bonn and Nijmegenl two-body effective nucleon-nucleon interactions. The sing...The shell model calculations in the sdgh major shell for the neutron-deficient ^106,107,108,109Sn isotopes have been carried out by using CD-Bonn and Nijmegenl two-body effective nucleon-nucleon interactions. The singleshell states and the corresponding matrix elements needed for describing Sn isotopes are reconstructed to calculate the coefficient of fractional parantage by reducing the calculation requirements. This reconstruction allows us to do the shell model calculations of the neutron deficient Sn isotopes in very reasonable time. The results are compared to the recent high-resolution experimental data and found to be in good agreement with experiments.展开更多
The three-body force effects on the equation of state and its iso-spin dependence of asymmetric nuclear matter and on the proton fraction in neutron star matter have been investigated within Brueckner-Hartree-Fock app...The three-body force effects on the equation of state and its iso-spin dependence of asymmetric nuclear matter and on the proton fraction in neutron star matter have been investigated within Brueckner-Hartree-Fock approach by using a microscopic three-body force. It is shown that, even in the presence of the three-body force, the empirical parabolic law of the energy per nucleon vs. isospin asymmetry is fulfilled in the whole asymmetry range and also up to high density. The three-body force provides a strong enhancement of symmetry energy at high density in agreement with relativistic approaches. It also shows that the three-body force leads to a much more rapid increasing of symmetry energy with density in relatively high density region and to a much lower threshold density for the direct URCA process to occur in a neutron star as compared to the predictions adopting only pure two-body force.展开更多
In the present work,we study the effect of the isovector scalar meson a_(0)(980)(also calledδ)to the asymmetric matter properties by constructing a parity doublet model with including the a_(0)(980)meson based on the...In the present work,we study the effect of the isovector scalar meson a_(0)(980)(also calledδ)to the asymmetric matter properties by constructing a parity doublet model with including the a_(0)(980)meson based on the approximate chiral U(2)_(L)×U(2)_(R)symmetry.The effect of a_(0)(980)to the properties of symmetric matter such as symmetry energy S(n_(B)),symmetry incompressibility K_(sym),symmetry skewness Q_(sym)is investigated.We find that,a_(0)(980)meson has significant effect to these nuclear matter properties especially when the chiral invariant mass of nucleon m_(0)is small.We also study the effect of the a_(0)(980)meson to the neutron star(NS)properties and find that it increase the radius of NSs,especially those having intermediate mass.Finally,we further constrain the value of the chiral invariant mass of the nucleon to 640 MeV≲m_(0)≲860 MeV for L_(0)=57:7MeV by comparing with the recent accepted value of K_(sym)and neutron star(NS)observational data.展开更多
文摘Previous research on gene expression analysis and association tests have suggested that RELN is a risk gene for schizophrenia in world populations.Based on the reported down-regulation of RELN in schizophrenia patients compared with normal subjects,we speculated that variants in the RELN promoter region may confer risk for schizophrenia.In this study,we investigated the associations of three SNPs in the promoter region of RELN with schizophrenia in a case-control sample from southwestern China（940 cases and 13 69 controls）.The results suggested that none of the SNPs showed significant associations in our sample,indicating the risk variants for schizophrenia in RELN may not be located in the promoter region.We also performed meta-analysis by combining our data with previously reported data on the Chinese population with a total sample size of 2 843 individuals,and the result remained nonsignificant.Collectively,our results suggested variants in the RELN promoter may not harbor risk SNPs associated with schizophrenia in the Chinese population.
文摘Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B （LMB）, a specific inhibitor of Crml, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals （NESs） in pericentrin with diverse export activities. All of the five NESs could bind to Crml in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal （NLS） consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin β. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.
基金Supported by the Key Program of the National Natural ScienceFoundation of China(No.30730071)the National High-Tech Researchand Development Program of China(863 program)(No.2007AA09Z430)the General Program of National Natural Science Foundation of China(Nos.30471347&40706048)
文摘Chinese shrimp （Fenneropenaeus chinensis） is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization （FISH） was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene （rDNA） of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of homologous chromosomes in F chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in E chinensis. The successful application ofFISH in E chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes ofpenaeid shrimps.
基金supported in part by the Scientific and Technological Council of Turkey under Grant No.TUBITAK 105T092Süleyman Demirel University under Grant No.SDUBAP 1075-m-05
文摘The shell model calculations in the sdgh major shell for the neutron-deficient ^106,107,108,109Sn isotopes have been carried out by using CD-Bonn and Nijmegenl two-body effective nucleon-nucleon interactions. The singleshell states and the corresponding matrix elements needed for describing Sn isotopes are reconstructed to calculate the coefficient of fractional parantage by reducing the calculation requirements. This reconstruction allows us to do the shell model calculations of the neutron deficient Sn isotopes in very reasonable time. The results are compared to the recent high-resolution experimental data and found to be in good agreement with experiments.
文摘The three-body force effects on the equation of state and its iso-spin dependence of asymmetric nuclear matter and on the proton fraction in neutron star matter have been investigated within Brueckner-Hartree-Fock approach by using a microscopic three-body force. It is shown that, even in the presence of the three-body force, the empirical parabolic law of the energy per nucleon vs. isospin asymmetry is fulfilled in the whole asymmetry range and also up to high density. The three-body force provides a strong enhancement of symmetry energy at high density in agreement with relativistic approaches. It also shows that the three-body force leads to a much more rapid increasing of symmetry energy with density in relatively high density region and to a much lower threshold density for the direct URCA process to occur in a neutron star as compared to the predictions adopting only pure two-body force.
文摘In the present work,we study the effect of the isovector scalar meson a_(0)(980)(also calledδ)to the asymmetric matter properties by constructing a parity doublet model with including the a_(0)(980)meson based on the approximate chiral U(2)_(L)×U(2)_(R)symmetry.The effect of a_(0)(980)to the properties of symmetric matter such as symmetry energy S(n_(B)),symmetry incompressibility K_(sym),symmetry skewness Q_(sym)is investigated.We find that,a_(0)(980)meson has significant effect to these nuclear matter properties especially when the chiral invariant mass of nucleon m_(0)is small.We also study the effect of the a_(0)(980)meson to the neutron star(NS)properties and find that it increase the radius of NSs,especially those having intermediate mass.Finally,we further constrain the value of the chiral invariant mass of the nucleon to 640 MeV≲m_(0)≲860 MeV for L_(0)=57:7MeV by comparing with the recent accepted value of K_(sym)and neutron star(NS)observational data.
