[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone (GH) protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base...[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone (GH) protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.展开更多
The paper proposes a unified framework to combine the advantages of the fast one-at-a-time approach and the high-performance all-at-once approach to perform Chinese Word Segmentation(CWS) and Part-of-Speech(PoS) taggi...The paper proposes a unified framework to combine the advantages of the fast one-at-a-time approach and the high-performance all-at-once approach to perform Chinese Word Segmentation(CWS) and Part-of-Speech(PoS) tagging.In this framework,the input of the PoS tagger is a candidate set of several CWS results provided by the CWS model.The widely used one-at-a-time approach and all-at-once approach are two extreme cases of the proposed candidate-based approaches.Experiments on Penn Chinese Treebank 5 and Tsinghua Chinese Treebank show that the generalized candidate-based approach outperforms one-at-a-time approach and even the all-at-once approach.The candidate-based approach is also faster than the time-consuming all-at-once approach.The authors compare three different methods based on sentence,words and character-intervals to generate the candidate set.It turns out that the word-based method has the best performance.展开更多
This paper deals with all aspects of liver alveolar echinococcosis (AE) including epidemiology, pathology, clinical manifestations, imaging examinations, diagnosis and differential diagnosis, surgical treatment and ch...This paper deals with all aspects of liver alveolar echinococcosis (AE) including epidemiology, pathology, clinical manifestations, imaging examinations, diagnosis and differential diagnosis, surgical treatment and chemotherapy.The review is not only based on personal clinical experiences but also in combination with relative basic research such as proliferation and growth of alveococcus, preclinical studies of a novel compound extracted from TCM for treatment of liver AE, and molecular immunology used for specific AE diagnosis, etc.展开更多
In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and ob...In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and obtained a transcript of 1 075 bp consisting of 1 37 bp 5′ untranslated region(UTR),515 bp 3′ UTR and a 423 bp open reading frame(ORF) encoding a polypeptide of 140 amino acid residues(GenBank accession number: KF359945).Homolog analysis showed a 70%–96% homology with sterol carrier protein-2(SCP-2) present in other animals,which is implicated in lipid metabolism of other organisms.The gene SCP-2 of Chinese toad(B.gargarizans) was cloned from a first strand cDNA of Bufo skin(GenBank accession number: KF381341) via PCR,whose encoding polypeptide has only one amino acid difference from that of Japanese toad.Tissue distribution analysis showed that SCP-2 expressed in all organs tested,though in the liver and spleen it manifested lower expression than in other organs.These findings might indicate SCP-2 being one of the active ingredients in toad skin.These findings may in turn have implications for further drug development from traditional Chinese medicine sources.展开更多
Long-term change of sea surface temperature (SST) in the China Seas from 1900 to 2006 is examined based on two different observation datasets (HadlSSTI and HadSST3). Similar to the Atlantic, SST in the China Seas ...Long-term change of sea surface temperature (SST) in the China Seas from 1900 to 2006 is examined based on two different observation datasets (HadlSSTI and HadSST3). Similar to the Atlantic, SST in the China Seas has been well observed during the past 107 years. A comparison between the reconstructed (HadISSTI) and un-interpolated (HadSST3) datasets shows that the SST wanning trends from both datasets are consistent with each other in most of the China Seas. The warming trends are stronger in winter than in summer, with a maximum rate of SST increase exceeding 2.7℃ (100year)-I in the East China Sea and the Taiwan Strait during winter based on HadISSTI. However, the SST from both datasets experienced a sudden decrease after 1999 in the China Seas. The estimated trend from HadlSSTI is stronger than that fi'om HadSST3 in the East China Sea and the east of Taiwan Island, where the difference in the linear SST warming trends are as large as about 1℃ (100year)-I when using respectively HadISST1 and HadSST3 datasets. When compared to the linear winter warnling trend of the land surface air temperature (1.6℃ (100 year)-1), HadSST3 shows a more reasonable trend of less than 2.1℃( 100 year)-1 than HadISST 1 's trend of larger than 2.7℃ ( 100 year)-1 at the mouth of the Yangtze River. The restllts also indicate large uncertainties in the estimate of SST warming patterns.展开更多
Long chain alkyl diols have shown important potential for the reconstruction of sea surface temperature,productivities and upwelling conditions in marine or lacustrine environments.However,little is known about the di...Long chain alkyl diols have shown important potential for the reconstruction of sea surface temperature,productivities and upwelling conditions in marine or lacustrine environments.However,little is known about the distribution and sources of the diols in eastern China marginal seas(CMS),which are areas of important organic carbon sink.Here the contents of C_(30) and C_(32)1,15-diols were analyzed in 181 surface sediments from eastern CMS.The similar distribution pattern and strong linear correlation between C_(30) and C_(32) diols indicated that they had similar biological source,with a dominance of C_(30) diol.Their contents ranged from 7-2726 ng g^(-1) for C_(30) diol and 5-669 ng g^(-1) for C_(32) diol,and both showed higher values mainly in the mud area of the Yellow Sea,while the TOC normalized contents showed a more obvious seaward increasing trend.The similar distribution pattern and significant positive correlation between diols and the other marine biomarkers(brassicasterol,dinosterol,C_(37) alkenones) indicated C_(30) and C_(32) diols in eastern CMS were mainly from marine algae.This conclusion was also supported by principal component analysis(PCA).Our results also showed that sediment diol contents were generally related to marine productivity,suggesting that diols could be applied for marine productivity reconstruction in eastern CMS.展开更多
Global long-term emission reduction targets need well defined options for equitable allocation of greenhouse gas emissions. Scholars from developing countries put forward the concept of equitable per capita cumulative...Global long-term emission reduction targets need well defined options for equitable allocation of greenhouse gas emissions. Scholars from developing countries put forward the concept of equitable per capita cumulative emission rights. There are four possible operational definitions resulting from this concept. These potential options for allocation of emission rights are expressed with mathematical equations. Through simple simulation, this paper reveals the advantages, disadvantages and characteristics of each option.展开更多
Various satellite data,JRA-25(Japan reanalysis of 25 years) reanalyzed data and WRF(Weather Research Forecast) model are used to investigate the in situ effect of the ESKF(East China Sea Kuroshio Front) on the MABL(ma...Various satellite data,JRA-25(Japan reanalysis of 25 years) reanalyzed data and WRF(Weather Research Forecast) model are used to investigate the in situ effect of the ESKF(East China Sea Kuroshio Front) on the MABL(marine atmospheric boundary layer).The intensity of the ESKF is most robust from January to April in its annual cycle.The local strong surface northerly/northeasterly winds are observed right over the ESKF in January and in April and the wind speeds decrease upward in the MABL.The thermal wind effect that is derived from the baroclinic MABL forced by the strong SST gradient contributes to the strong surface winds to a large degree.The convergence zone existing along the warm flank of the ESKF is stronger in April than in January corresponding to the steeper SST(sea surface temperature) gradient.The collocations of the cloud cover maximum and precipitation maximum are basically consistent with the convergence zone of the wind field.The clouds develop higher(lower) in the warm(cold) flank of the ESKF due to the less(more) stable stratification in the MABL.The lowest clouds are observed in April on the cold flank of the ESKF and over the Yellow Sea due to the existence of the pronounced temperature inversion.The numerical experiments with smoothed SST are consistent with the results from the ovservations.展开更多
Recently, there have been many mo- bile value-added services in the Chinese mo- bile telecommunication market nowadays. Am- ong them, the characteristics of Multimedia Mes- saging Service (MMS) have not yet been ful...Recently, there have been many mo- bile value-added services in the Chinese mo- bile telecommunication market nowadays. Am- ong them, the characteristics of Multimedia Mes- saging Service (MMS) have not yet been fully understood. In this paper, with the help of a cloud computing platform, we investigated the flow-level charactefistcs of Chinese MMS. All of the experimental data were collected by the TMS equipment deployed in a major node in Sou- them China. The collection time spanned six mo- nths. We performed high-level analysis to show the basic distributions of MMS characteristics. Then, by analysing the detailed MMS features, we determined the distribution of personal MMS, and made a comprehensive comparison between 2G and 3G MMS. Finally, we tried to build a model on the personal MMS inter-arrival time, and we found that the Weibull distribution was optimum.展开更多
Two species in the genus Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae) from China, A. callicarpae Takahashi and A. styraci Takahashi are reported in this paper. The diagnosis, distribution, host plant and morpholo...Two species in the genus Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae) from China, A. callicarpae Takahashi and A. styraci Takahashi are reported in this paper. The diagnosis, distribution, host plant and morphological illustrations of these two species are provided. An identification key to the puparia of Chinese Acanthaleyrodes species is given.展开更多
文摘[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone (GH) protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.
基金supported by the National Natural Science Foundation of China under GrantNo.60873174
文摘The paper proposes a unified framework to combine the advantages of the fast one-at-a-time approach and the high-performance all-at-once approach to perform Chinese Word Segmentation(CWS) and Part-of-Speech(PoS) tagging.In this framework,the input of the PoS tagger is a candidate set of several CWS results provided by the CWS model.The widely used one-at-a-time approach and all-at-once approach are two extreme cases of the proposed candidate-based approaches.Experiments on Penn Chinese Treebank 5 and Tsinghua Chinese Treebank show that the generalized candidate-based approach outperforms one-at-a-time approach and even the all-at-once approach.The candidate-based approach is also faster than the time-consuming all-at-once approach.The authors compare three different methods based on sentence,words and character-intervals to generate the candidate set.It turns out that the word-based method has the best performance.
文摘This paper deals with all aspects of liver alveolar echinococcosis (AE) including epidemiology, pathology, clinical manifestations, imaging examinations, diagnosis and differential diagnosis, surgical treatment and chemotherapy.The review is not only based on personal clinical experiences but also in combination with relative basic research such as proliferation and growth of alveococcus, preclinical studies of a novel compound extracted from TCM for treatment of liver AE, and molecular immunology used for specific AE diagnosis, etc.
基金Foundation items: This study was supported by the National Natural Science Foundation of China (31071181, 31372149) and the Students' Innovative Training Program of ZAFU (20120207, 20120213)
文摘In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and obtained a transcript of 1 075 bp consisting of 1 37 bp 5′ untranslated region(UTR),515 bp 3′ UTR and a 423 bp open reading frame(ORF) encoding a polypeptide of 140 amino acid residues(GenBank accession number: KF359945).Homolog analysis showed a 70%–96% homology with sterol carrier protein-2(SCP-2) present in other animals,which is implicated in lipid metabolism of other organisms.The gene SCP-2 of Chinese toad(B.gargarizans) was cloned from a first strand cDNA of Bufo skin(GenBank accession number: KF381341) via PCR,whose encoding polypeptide has only one amino acid difference from that of Japanese toad.Tissue distribution analysis showed that SCP-2 expressed in all organs tested,though in the liver and spleen it manifested lower expression than in other organs.These findings might indicate SCP-2 being one of the active ingredients in toad skin.These findings may in turn have implications for further drug development from traditional Chinese medicine sources.
基金supported by the National Basic Research Program of China(2012-CB955602)National Key Program for Developing Basic Science(2010CB428904)Natural Science Foundation of China(40830106,40921004 and 41176006)
文摘Long-term change of sea surface temperature (SST) in the China Seas from 1900 to 2006 is examined based on two different observation datasets (HadlSSTI and HadSST3). Similar to the Atlantic, SST in the China Seas has been well observed during the past 107 years. A comparison between the reconstructed (HadISSTI) and un-interpolated (HadSST3) datasets shows that the SST wanning trends from both datasets are consistent with each other in most of the China Seas. The warming trends are stronger in winter than in summer, with a maximum rate of SST increase exceeding 2.7℃ (100year)-I in the East China Sea and the Taiwan Strait during winter based on HadISSTI. However, the SST from both datasets experienced a sudden decrease after 1999 in the China Seas. The estimated trend from HadlSSTI is stronger than that fi'om HadSST3 in the East China Sea and the east of Taiwan Island, where the difference in the linear SST warming trends are as large as about 1℃ (100year)-I when using respectively HadISST1 and HadSST3 datasets. When compared to the linear winter warnling trend of the land surface air temperature (1.6℃ (100 year)-1), HadSST3 shows a more reasonable trend of less than 2.1℃( 100 year)-1 than HadISST 1 's trend of larger than 2.7℃ ( 100 year)-1 at the mouth of the Yangtze River. The restllts also indicate large uncertainties in the estimate of SST warming patterns.
基金supported by the National Natural Science Foundation of China(Nos.41521064 and 41630966)
文摘Long chain alkyl diols have shown important potential for the reconstruction of sea surface temperature,productivities and upwelling conditions in marine or lacustrine environments.However,little is known about the distribution and sources of the diols in eastern China marginal seas(CMS),which are areas of important organic carbon sink.Here the contents of C_(30) and C_(32)1,15-diols were analyzed in 181 surface sediments from eastern CMS.The similar distribution pattern and strong linear correlation between C_(30) and C_(32) diols indicated that they had similar biological source,with a dominance of C_(30) diol.Their contents ranged from 7-2726 ng g^(-1) for C_(30) diol and 5-669 ng g^(-1) for C_(32) diol,and both showed higher values mainly in the mud area of the Yellow Sea,while the TOC normalized contents showed a more obvious seaward increasing trend.The similar distribution pattern and significant positive correlation between diols and the other marine biomarkers(brassicasterol,dinosterol,C_(37) alkenones) indicated C_(30) and C_(32) diols in eastern CMS were mainly from marine algae.This conclusion was also supported by principal component analysis(PCA).Our results also showed that sediment diol contents were generally related to marine productivity,suggesting that diols could be applied for marine productivity reconstruction in eastern CMS.
基金supported by the 2009 special study project employing basic scientific research fund of the Academy of Macroeconomic Research of NDRC
文摘Global long-term emission reduction targets need well defined options for equitable allocation of greenhouse gas emissions. Scholars from developing countries put forward the concept of equitable per capita cumulative emission rights. There are four possible operational definitions resulting from this concept. These potential options for allocation of emission rights are expressed with mathematical equations. Through simple simulation, this paper reveals the advantages, disadvantages and characteristics of each option.
基金supported by the National Natural Science Foundation of China (No.40975003)the Ph.D.Programs Foundation of Ministry of Education of China (No.20090132110008)GYHY(QX)2007-6-31
文摘Various satellite data,JRA-25(Japan reanalysis of 25 years) reanalyzed data and WRF(Weather Research Forecast) model are used to investigate the in situ effect of the ESKF(East China Sea Kuroshio Front) on the MABL(marine atmospheric boundary layer).The intensity of the ESKF is most robust from January to April in its annual cycle.The local strong surface northerly/northeasterly winds are observed right over the ESKF in January and in April and the wind speeds decrease upward in the MABL.The thermal wind effect that is derived from the baroclinic MABL forced by the strong SST gradient contributes to the strong surface winds to a large degree.The convergence zone existing along the warm flank of the ESKF is stronger in April than in January corresponding to the steeper SST(sea surface temperature) gradient.The collocations of the cloud cover maximum and precipitation maximum are basically consistent with the convergence zone of the wind field.The clouds develop higher(lower) in the warm(cold) flank of the ESKF due to the less(more) stable stratification in the MABL.The lowest clouds are observed in April on the cold flank of the ESKF and over the Yellow Sea due to the existence of the pronounced temperature inversion.The numerical experiments with smoothed SST are consistent with the results from the ovservations.
基金supported in part by the National Science and Technology Major Project under Grant No.2012ZX03002008the National Natural Science Foundation of China under Grant No.61072061the National "111" Project of China’s Higher Education under Grant No.B08004
文摘Recently, there have been many mo- bile value-added services in the Chinese mo- bile telecommunication market nowadays. Am- ong them, the characteristics of Multimedia Mes- saging Service (MMS) have not yet been fully understood. In this paper, with the help of a cloud computing platform, we investigated the flow-level charactefistcs of Chinese MMS. All of the experimental data were collected by the TMS equipment deployed in a major node in Sou- them China. The collection time spanned six mo- nths. We performed high-level analysis to show the basic distributions of MMS characteristics. Then, by analysing the detailed MMS features, we determined the distribution of personal MMS, and made a comprehensive comparison between 2G and 3G MMS. Finally, we tried to build a model on the personal MMS inter-arrival time, and we found that the Weibull distribution was optimum.
基金funded by the National Natural Science Foundation of China (31601884)the Research and Development Fund of Zhejiang A&F University (2017FR041)
文摘Two species in the genus Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae) from China, A. callicarpae Takahashi and A. styraci Takahashi are reported in this paper. The diagnosis, distribution, host plant and morphological illustrations of these two species are provided. An identification key to the puparia of Chinese Acanthaleyrodes species is given.