Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nemato...Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced betw een two sexes and get basic data on genetic development, we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly, throug h SD S-PAGE, we found more than three specific proteins were detected in female imag o with the approximate molecular weight of 15-16 kD, 21-24 kD, 72-76 kD and on e abou t 26 kD specific protein in male imago. Secondly, we used a new improved two-di me nsional electrophoresis method to analyze the proteins and found that the result acco rds with those of SDS-PAGE, which showed that this 2-DE method is practicable. A lso, in this 2-DE, proteins can be well separated and plenty of different pr oteins can be clearly displayed .展开更多
1996—1997年,在福州,用中华卵索线虫Ovomermis sinensis(Chen et al)侵染期幼虫对当地主要蔬菜害虫,菜青虫、斜纹夜蛾和甜菜夜蛾等幼虫,在室内做人工感染试验。在室温20—28℃,寄生线虫与宿主昆虫的比例为为80:1的情况下,线虫对菜青虫...1996—1997年,在福州,用中华卵索线虫Ovomermis sinensis(Chen et al)侵染期幼虫对当地主要蔬菜害虫,菜青虫、斜纹夜蛾和甜菜夜蛾等幼虫,在室内做人工感染试验。在室温20—28℃,寄生线虫与宿主昆虫的比例为为80:1的情况下,线虫对菜青虫、斜纹夜蛾和甜菜夜蛾幼虫的寄生率分别为50％,94％,92％。在当地土壤中无中华卵索线虫的空心菜田,设三个小区,每小区15m^2,各移植斜纹夜蛾2—3龄幼虫150头于菜株上,A区用12540条线虫侵染期幼虫混在2460克湿沙中,撤施于植株根部土面;B区用同样数量的线虫混在8公斤水中,喷洒在菜株上;C区为不释放线虫的对照区。于释放线虫后的第4天和第7天,从各小区随机回收斜纹夜蛾幼虫20头和30头,在室内单头饲养,检查线虫的寄生率和寄生强度。结果:第4天调查寄生效果相近,寄生率分别为61.1％,60％。第7天调查寄生率分别为76.7％,70％。而对照区的虫体中均未见有线虫寄生,寄生率为0。差别显著。在元白菜上释放中华卵索线虫侵染期幼虫,莱青虫的寄生率为6.6％。展开更多
文摘Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced betw een two sexes and get basic data on genetic development, we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly, throug h SD S-PAGE, we found more than three specific proteins were detected in female imag o with the approximate molecular weight of 15-16 kD, 21-24 kD, 72-76 kD and on e abou t 26 kD specific protein in male imago. Secondly, we used a new improved two-di me nsional electrophoresis method to analyze the proteins and found that the result acco rds with those of SDS-PAGE, which showed that this 2-DE method is practicable. A lso, in this 2-DE, proteins can be well separated and plenty of different pr oteins can be clearly displayed .
文摘1996—1997年,在福州,用中华卵索线虫Ovomermis sinensis(Chen et al)侵染期幼虫对当地主要蔬菜害虫,菜青虫、斜纹夜蛾和甜菜夜蛾等幼虫,在室内做人工感染试验。在室温20—28℃,寄生线虫与宿主昆虫的比例为为80:1的情况下,线虫对菜青虫、斜纹夜蛾和甜菜夜蛾幼虫的寄生率分别为50％,94％,92％。在当地土壤中无中华卵索线虫的空心菜田,设三个小区,每小区15m^2,各移植斜纹夜蛾2—3龄幼虫150头于菜株上,A区用12540条线虫侵染期幼虫混在2460克湿沙中,撤施于植株根部土面;B区用同样数量的线虫混在8公斤水中,喷洒在菜株上;C区为不释放线虫的对照区。于释放线虫后的第4天和第7天,从各小区随机回收斜纹夜蛾幼虫20头和30头,在室内单头饲养,检查线虫的寄生率和寄生强度。结果:第4天调查寄生效果相近,寄生率分别为61.1％,60％。第7天调查寄生率分别为76.7％,70％。而对照区的虫体中均未见有线虫寄生,寄生率为0。差别显著。在元白菜上释放中华卵索线虫侵染期幼虫,莱青虫的寄生率为6.6％。
