Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated usi...Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.展开更多
It is necessary to master certain scientific methods in studying the historical experience of the People＇s Republic of China; that is, we should study the experience of different periods of New China and at the same ...It is necessary to master certain scientific methods in studying the historical experience of the People＇s Republic of China; that is, we should study the experience of different periods of New China and at the same time study the experience of various historical periods as related to one another; we should study the successful experience of New China and at the same time study its errors and setbacks; and we should view the historical experience of New China from today＇s perspective and at the same time study it by placing it under given historical conditions.展开更多
文摘Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
文摘It is necessary to master certain scientific methods in studying the historical experience of the People＇s Republic of China; that is, we should study the experience of different periods of New China and at the same time study the experience of various historical periods as related to one another; we should study the successful experience of New China and at the same time study its errors and setbacks; and we should view the historical experience of New China from today＇s perspective and at the same time study it by placing it under given historical conditions.
