Objective To explore the association between the three polymorphisms [ C825T, C1429T and G（-350）A] of the gene encoding the G protein beta 3 subunit （GNB3） and hypertension by performing a case-control study in th...Objective To explore the association between the three polymorphisms [ C825T, C1429T and G（-350）A] of the gene encoding the G protein beta 3 subunit （GNB3） and hypertension by performing a case-control study in the northern Han Chinese population. Methods We recnaited 731 hypertensive patients and 673 control subjects （the calculated power value was 〉 0.8）. Genotyping was performed to identify C825T, C1429T and G（-350）A polymorphisms using the TaqMan assay. Comparisons of allelic and genotypic frequencies between cases and controls were made by using the chi-square test. Logistic regression analyses were performed to investigate the relationships between the three polymorphisms of GNB3 gene under different genetic models （additive, dominant and recessive models）. Results The genotype dis- tribution and allele frequencies of C825T, C1429T and G（-350）A polymorphisms did not differ significantly between hypertensive patients and control subjects, either when the full sample was assessed, or when the sample was stratified by gender. No significant association was observed between C825T, C 1429T and G（-350）A polymorphisms and the risk of essential hypertension in any genetic model. Linkage dis- equilibrium was only detected between C825T and C 1429T polymorphisms. Haplotype analyses observed that none of the three estimated haplotypes significantly increased the risk of hypertension. Conclusions Our study suggested that the GNB3 gene polymorphisms [C825T, C 1429T and G（-350）A] were not significantly associated with essential hypertension in northern Han Chinese population.展开更多
Objective. Atopic asthma provides a useful model for evaluating the genetic factors that control human immune responsiveness. HLA class Ⅱ gene products are involved in the control of immune response. As HLA-DRB gene ...Objective. Atopic asthma provides a useful model for evaluating the genetic factors that control human immune responsiveness. HLA class Ⅱ gene products are involved in the control of immune response. As HLA-DRB gene is the most polymorphic HLA class Ⅱ gene, we investigated whether susceptibility or resistance to the disease is associated with HLA-DRB. Methods. Blood samples were obtained from two groups of unrelated Chinese northern adults: （l） 50 atopic asthma （7 of them with familial aggregation） ;（2） 80 healthy controls without asthma or atopy and other HLA-associated diseases. Genomic DNA was extracted from peripheral venous blood leucocytes. The polymorphic second exon of HLA-DRB gene was amplified by sequence-specific primer polymerase chain reaction （SSP/PCR） methods. All patients had their serum IgE （total and specific） antibody levels by RAST, bronchial reactivity assessed by methacholine brocho-provocation test and/or bronchodilation test. Results. There was an increased gene frequency of DR6（13） and DR52 in asthmatic subjects compared with healthy subjects（17% vs 4. 3%, P<0.01; 50% vs 17. 5 %, P<0. 01 ）, and the decreased frequency of DR2（15） and DR51 in asthmatic patients（7% vs 18%, P<0. 05; 2 % vs 33 %, P<0. 01 ）. We found the positive association between DR6（13）-DR52 and sIgE antibody responsiveness to dl （from house dust mite al- lergen ）; negative association between HLA-DRB alleles and TIgE or BHR （bronchial hyperresponsiveness ）. Conclusion. The results suggested that HLA haplotype DR6（13）-DR52 was significantly implicated in sus- ceptibility to house dust mite induced-asthma, at least it would be more closely associated with atopic asth- ma. Conversely, alleles DR2（15） and DR51 might confer protection against the disease. HLA-DRB genes were particularly involved in regulating human atopic immune response in asthma.展开更多
Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 ...Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population,this seroepidemiological study was conducted.Methods. Sera were collected from 430 healthy persons and donors living in North China,and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer e-qual or higher than 1:10 was regarded as seropositive.Results. Of the 430 serum samples tested,297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males,with X2 = 7. 05,P<0. 01 and F = 7.23,P<0. 01,respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes,with X2=20. 08 and 20. 28,P = 0. 04,respectively,and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes.Conclusions. This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China,and its importance should be further studied.展开更多
The height of 26,940 Chinese Han adults （16,503 rural and 10,437 urban adults） from 11 Han ethnic groups was measured and analyzed in the current survey. The top three highest dialect groups in rural Han populations...The height of 26,940 Chinese Han adults （16,503 rural and 10,437 urban adults） from 11 Han ethnic groups was measured and analyzed in the current survey. The top three highest dialect groups in rural Han populations are Jianghuai （male 167.3± 6.4 cm, female 156.5 ±5.6 cm）, North China （male 167.3 ± 6.4 cm, female 155.7± 5.7 cm）, and Wu （male 166.7 ± 6.9 cm, female 155.6 ± 5.9 cm） groups. In urban Han populations, the top three groups are as follows： the Northeast China （male 169.5 ±6.7 cm, female 158.0 ±6.1 cm）, North China （male 168.5 ± 6.2 cm, female 157.3±5.8 cm）, and Jianghuai （male 169.2 ± 6.2cm, female 157.1 ± 5.6 cm） dialect groups. The Gan dialect group （male 164.0 ± 6.3 cm, female 153.9±5.0 cm） was the shortest in both rural and urban groups. The different stature of Han dialect groups may be a result of interaction between genetic background and different environmental factors, labor intensity, diet composition and nutrition intake in different areas in China.展开更多
基金grants of the National High Technology Research and Development Program，grants of the National Eleventh Five-year Plan Program from the Ministry of Science and Technology of China，Beijing Natural Science Foundation
文摘Objective To explore the association between the three polymorphisms [ C825T, C1429T and G（-350）A] of the gene encoding the G protein beta 3 subunit （GNB3） and hypertension by performing a case-control study in the northern Han Chinese population. Methods We recnaited 731 hypertensive patients and 673 control subjects （the calculated power value was 〉 0.8）. Genotyping was performed to identify C825T, C1429T and G（-350）A polymorphisms using the TaqMan assay. Comparisons of allelic and genotypic frequencies between cases and controls were made by using the chi-square test. Logistic regression analyses were performed to investigate the relationships between the three polymorphisms of GNB3 gene under different genetic models （additive, dominant and recessive models）. Results The genotype dis- tribution and allele frequencies of C825T, C1429T and G（-350）A polymorphisms did not differ significantly between hypertensive patients and control subjects, either when the full sample was assessed, or when the sample was stratified by gender. No significant association was observed between C825T, C 1429T and G（-350）A polymorphisms and the risk of essential hypertension in any genetic model. Linkage dis- equilibrium was only detected between C825T and C 1429T polymorphisms. Haplotype analyses observed that none of the three estimated haplotypes significantly increased the risk of hypertension. Conclusions Our study suggested that the GNB3 gene polymorphisms [C825T, C 1429T and G（-350）A] were not significantly associated with essential hypertension in northern Han Chinese population.
基金State Science and Technology Commission of China !(No. 96-906-02-04)Glaxowellcome Ltd.
文摘Objective. Atopic asthma provides a useful model for evaluating the genetic factors that control human immune responsiveness. HLA class Ⅱ gene products are involved in the control of immune response. As HLA-DRB gene is the most polymorphic HLA class Ⅱ gene, we investigated whether susceptibility or resistance to the disease is associated with HLA-DRB. Methods. Blood samples were obtained from two groups of unrelated Chinese northern adults: （l） 50 atopic asthma （7 of them with familial aggregation） ;（2） 80 healthy controls without asthma or atopy and other HLA-associated diseases. Genomic DNA was extracted from peripheral venous blood leucocytes. The polymorphic second exon of HLA-DRB gene was amplified by sequence-specific primer polymerase chain reaction （SSP/PCR） methods. All patients had their serum IgE （total and specific） antibody levels by RAST, bronchial reactivity assessed by methacholine brocho-provocation test and/or bronchodilation test. Results. There was an increased gene frequency of DR6（13） and DR52 in asthmatic subjects compared with healthy subjects（17% vs 4. 3%, P<0.01; 50% vs 17. 5 %, P<0. 01 ）, and the decreased frequency of DR2（15） and DR51 in asthmatic patients（7% vs 18%, P<0. 05; 2 % vs 33 %, P<0. 01 ）. We found the positive association between DR6（13）-DR52 and sIgE antibody responsiveness to dl （from house dust mite al- lergen ）; negative association between HLA-DRB alleles and TIgE or BHR （bronchial hyperresponsiveness ）. Conclusion. The results suggested that HLA haplotype DR6（13）-DR52 was significantly implicated in sus- ceptibility to house dust mite induced-asthma, at least it would be more closely associated with atopic asth- ma. Conversely, alleles DR2（15） and DR51 might confer protection against the disease. HLA-DRB genes were particularly involved in regulating human atopic immune response in asthma.
文摘Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population,this seroepidemiological study was conducted.Methods. Sera were collected from 430 healthy persons and donors living in North China,and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer e-qual or higher than 1:10 was regarded as seropositive.Results. Of the 430 serum samples tested,297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males,with X2 = 7. 05,P<0. 01 and F = 7.23,P<0. 01,respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes,with X2=20. 08 and 20. 28,P = 0. 04,respectively,and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes.Conclusions. This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China,and its importance should be further studied.
基金supported by the National Natural Science Foundation of China (30830062)
文摘The height of 26,940 Chinese Han adults （16,503 rural and 10,437 urban adults） from 11 Han ethnic groups was measured and analyzed in the current survey. The top three highest dialect groups in rural Han populations are Jianghuai （male 167.3± 6.4 cm, female 156.5 ±5.6 cm）, North China （male 167.3 ± 6.4 cm, female 155.7± 5.7 cm）, and Wu （male 166.7 ± 6.9 cm, female 155.6 ± 5.9 cm） groups. In urban Han populations, the top three groups are as follows： the Northeast China （male 169.5 ±6.7 cm, female 158.0 ±6.1 cm）, North China （male 168.5 ± 6.2 cm, female 157.3±5.8 cm）, and Jianghuai （male 169.2 ± 6.2cm, female 157.1 ± 5.6 cm） dialect groups. The Gan dialect group （male 164.0 ± 6.3 cm, female 153.9±5.0 cm） was the shortest in both rural and urban groups. The different stature of Han dialect groups may be a result of interaction between genetic background and different environmental factors, labor intensity, diet composition and nutrition intake in different areas in China.
