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中国白菜小孢子离体培养和双单倍体植株再生 被引量:3
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作者 陈琳 刘克德 +3 位作者 吴江生 孙秀丽 汪频 石淑稳 《中国农学通报》 CSCD 北大核心 2010年第14期78-81,共4页
采用略加修改的NLN培养基(无机大量元素1/2)和甘蓝型油菜小孢子的胚状体诱导程序,培养了5个中国菜用小白菜品种和1个中国大白菜品种的小孢子,进行了双单倍体的诱导。结果表明,不同品种的小孢子产胚量差异很大,在接种的6个基因型中,有5... 采用略加修改的NLN培养基(无机大量元素1/2)和甘蓝型油菜小孢子的胚状体诱导程序,培养了5个中国菜用小白菜品种和1个中国大白菜品种的小孢子,进行了双单倍体的诱导。结果表明,不同品种的小孢子产胚量差异很大,在接种的6个基因型中,有5个诱导出胚,诱导率83.33%。其中的中萁青抗热605产胚量最高,是8.68个胚/10蕾,最低的是鸡冠菜,其产胚率是0.42个胚/10蕾。这些胚在添加了NAA和PP333(多效唑)的B5培养基发育成健壮的小植株,已移栽到田间。关于6个基因型的小孢子胚胎发生能力,还有待进一步研究。 展开更多
关键词 中国大白菜 中国白菜 小孢子离体培养 植株再生
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Change of 3 Kinds of Enzyme Activity and Soluble Sugar in 3 Chinese Cabbage Varieties with Different Resistance Level During the Process of Plasmodiophora brassicae Infecting Their Roots 被引量:1
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作者 Zuohua REN Hu ZHOU +3 位作者 Xiaoli HUANG Qiong SONG Minjie LIU Erming LIU 《Agricultural Science & Technology》 CAS 2015年第2期205-208,共4页
There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activitie... There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activities of 3cultivars with different clubroot-resistant levelswere detected after inoculation in this study.The results suggest that the changing rates of CAT,PAL and SOD contents of resistance cultivars after inoculation were higher than those of susceptible cultivars(Baigengbaiye>Zaobaicaitai>Huangjinxiaobaicai);the POD activities of resistance cultivars reached a peak on an earlier day than those of susceptible cultivars;the increasing rate of soluble sugar of susceptible cultivars was higher than that of the resistance cultivars,and ended with two peaks.Therefore,the four enzymes(CAT,POD,PAL and SOD)and soluble sugar may be used as physiological and biochemical reference indexes for the resistance identification to clubroot after inoculation with P.brassicae. 展开更多
关键词 Chinese cabbage CLUBROOT Defensive enzyme Soluble sugar
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