Objectives To evaluate the association between a KCNQ 1 mutation, R259H, and short QT syndrome （SQTS） and to explore the elec- trophysiological mechanisms underlying their association. Methods We performed genetic s...Objectives To evaluate the association between a KCNQ 1 mutation, R259H, and short QT syndrome （SQTS） and to explore the elec- trophysiological mechanisms underlying their association. Methods We performed genetic screening of SQTS genes in 25 probands and their family members （63 patients）. We used direct sequencing to screen the exons and intron-exon boundaries of candidate genes that en- code ion channels which contribute to the repolarization of the ventricular action potential, including KCNQI, KCNH2, KCNE1, KCNE2, KCNJ2, CACNAlc, CACNB2b and CACNA2D1. In one of the 25 SQTS probands screened, we discovered a KCNQ1 mutation, R259H. We cloned R259H and transiently expressed it in HEK-293 cells; then, currents were recorded using whole cell patch clamp techniques. Results R259H-KCNQ 1 showed significantly increased current density, which was approximately 3-fold larger than that of wild type （WT） after a depolarizing pulse at 1 s. The steady state voltage dependence of the activation and inactivation did not show significant differences between the WT and R259H mutation （P 〉 0.05）, whereas the time constant of deactivation was markedly prolonged in the mutant compared with the WT in terms of the test potentials, which indicated that the deactivation of R259H was markedly slower than that of the WT. These results suggested that the R259H mutation can effectively increase the slowly activated delayed rectifier potassium current （Irs） in phase 3 of the cardiac action potential, which may be an infrequent cause of QT interval shortening. Conclusions R259H is a gain-of-function muta- tion of the KCNQ1 channel that is responsible for SQTS2. This is the first time that the R259H mutation was detected in Chinese people.展开更多
Objectives:The integrated model of frailty shifted the physiologically-dominated view to an integrated and multidisciplinary view of frailty and adopted an integrated and holistic approach to the complex challenges fa...Objectives:The integrated model of frailty shifted the physiologically-dominated view to an integrated and multidisciplinary view of frailty and adopted an integrated and holistic approach to the complex challenges facing frail older adults and their caregivers.There is an urgent need for researchers to identify multi-faceted frailty among older adults and to explore contributors to it.We assessed the level of frailty among older residents in long-term care(LTC)facilities in Shanghai,China and explored risk factors of multiple dimensions of frailty among older residents.Methods:It is a cross-sectional descriptive study.We interviewed 218 older residents from nine LTC facilities.We used Tilburg Frailty Indicator to assess older residents’physiological,psychological,and social frailty.The Geriatric Depression Scale,Mini-Mental state examination,Athens Insomnia Scale,and Activity of Daily Living(ADL)Scale were used to assess their depressive symptoms,cognitive function,sleep quality and ADL abilities,respectively.Results:Older residents have a moderate level of frailty in physiological,psychological and social domains.There is a high correlation among dimensions of frailty.Older residents’depressive symptoms have remained positively associated with their overall and each domain of frailty.Older residents’actual activity participation and exercise in LTC facilities are negatively associated with older residents’overall,physiological and social frailty.Conclusions:Frailty is a multi-dimensional concept and is prevalent among older residents in LTC facilities.It is important to take an integrated view on frailty and carry out appropriate and comprehensive interventions to prevent adverse outcomes and provide holistic care for frail older residents in LTC facilities.There is an urgent need to improve the quality and expand the number of activities offered within LTC facilities that would engage older residents in a person-centered way,despite their age,ADL abilities,frailty and functional limitations.展开更多
Reading strategies are different from identifying the words, but a kind of metacognitive activity based on the monitoring mode. This paper will explore Chinese English learners＇ text reading strategies according to t...Reading strategies are different from identifying the words, but a kind of metacognitive activity based on the monitoring mode. This paper will explore Chinese English learners＇ text reading strategies according to the cognitive process of the reading, hoping for providing some suggestions and references for Chinese college students to learn English well and improve the text reading ability.展开更多
Based on faults surveying and research data in the Tianjin offshore areas,through studying tectonic structure,Quaternary activity,deep structure,stress and strain fields and seismicity in the Tianjin offshore areas,th...Based on faults surveying and research data in the Tianjin offshore areas,through studying tectonic structure,Quaternary activity,deep structure,stress and strain fields and seismicity in the Tianjin offshore areas,the activity and tectonic features of the faults are determined synthetically.Using seismo-geological data,and the historical and modern seismicity data,the frequency-magnitude relationship model normalized by 500a is established and based on the relationship between the upper limit of maximum magnitude M u and a t/b,the maximum magnitudes of the sea section of the Haihe river fault and the Haiyi fault are calculated.Then Poisson probability model is adopted and the quantitative parameters,such as the maximum magnitude,occurrence probability,recurrence cycle of the faults in the south Tianjin offshore areas in the coming 50～200a,are calculated.展开更多
China Low Activation Martensitic （CLAM） steel is being studied to develop the structural materials for a fusion reactor, which has been designed based on the well-known 9Crl.5WVTa steel. The effect of tempering temp...China Low Activation Martensitic （CLAM） steel is being studied to develop the structural materials for a fusion reactor, which has been designed based on the well-known 9Crl.5WVTa steel. The effect of tempering temperature on hardness and micro- structure of CLAM steel was studied. The strength of CLAM steel increased by adding silicon, and the ductility remained con- stant. Conversely, while CLAM steel maintained good ductility with the addition of yttrium, its tensile strengths were greatly degraded. Behaviors under electron irradiation of CLAM steel were examined using the high voltage electron microscope. Electron irradiation at 450℃ formed many voids in CLAM steel with basic composition, whereas CLAM with silicon steel did not change the microstructure significantly.展开更多
Human activities are strongly modifying the global nitrogen (N) cycle through increasing input, N species diversity, and pool size of industrial reactive N (Nr). However, the fluxes, fates and environmental consequenc...Human activities are strongly modifying the global nitrogen (N) cycle through increasing input, N species diversity, and pool size of industrial reactive N (Nr). However, the fluxes, fates and environmental consequences of industrial Nr (excluding synthesized N fertilizer) remain poorly understood and quantified. We report here that industrial Nr flux has increased 13.4-fold over the past 30 years in China, reaching 3.7Tg N (1 Tg=10 12g) in 2008, accounting for over 50% of China's food Nr flux. Socioeconomic development (per capita GDP, urbanization and household size) significantly drives the growth of industrial Nr fluxes. This leads to "hotspots" of industrial Nr, mainly in relatively developed Eastern China. Industrial Nr loss rate during production is only 5%, much lower than that of cropland (50%) and livestock (80%). However, industrial Nr loss is point source pollution, and Nr release in concentrated doses produces serious risk in small regions. The contribution of structural N to total industrial Nr with a lifespan longer than one year (e.g., synthetic fiber, plastic) increased from 20% in 1980 to 70% in 2008. There was about 2.6 Tg N structural industrial Nr accumulated in human settlements in 2008, which could be one ex- planation of an unknown Nr sink of anthropogenic Nr input (mainly Haber-Bosch N fixation). Legacy effects caused by structural N accumulation have long-term consequences for environmental and human health, although structural N delays Nr release and reduces short-term Nr pollution. Industrial Nr use generates new features of modern global N biogeochemistry, such as increasing Nr species diversity, reducing Nr turnover rate. Future dynamics simulation of the earth system should involve industrial Nr. Explicit consideration and accounting of the fluxes and environmental consequences of industrial Nr would provide decision-makers a novel view of regional sustainable development.展开更多
基金grants obtained from the National Natural Science Foundation of China （No.： 81170177, 81030002） and science and Technology De- partment of Gansu Province Project （145RJZ104）.
文摘Objectives To evaluate the association between a KCNQ 1 mutation, R259H, and short QT syndrome （SQTS） and to explore the elec- trophysiological mechanisms underlying their association. Methods We performed genetic screening of SQTS genes in 25 probands and their family members （63 patients）. We used direct sequencing to screen the exons and intron-exon boundaries of candidate genes that en- code ion channels which contribute to the repolarization of the ventricular action potential, including KCNQI, KCNH2, KCNE1, KCNE2, KCNJ2, CACNAlc, CACNB2b and CACNA2D1. In one of the 25 SQTS probands screened, we discovered a KCNQ1 mutation, R259H. We cloned R259H and transiently expressed it in HEK-293 cells; then, currents were recorded using whole cell patch clamp techniques. Results R259H-KCNQ 1 showed significantly increased current density, which was approximately 3-fold larger than that of wild type （WT） after a depolarizing pulse at 1 s. The steady state voltage dependence of the activation and inactivation did not show significant differences between the WT and R259H mutation （P 〉 0.05）, whereas the time constant of deactivation was markedly prolonged in the mutant compared with the WT in terms of the test potentials, which indicated that the deactivation of R259H was markedly slower than that of the WT. These results suggested that the R259H mutation can effectively increase the slowly activated delayed rectifier potassium current （Irs） in phase 3 of the cardiac action potential, which may be an infrequent cause of QT interval shortening. Conclusions R259H is a gain-of-function muta- tion of the KCNQ1 channel that is responsible for SQTS2. This is the first time that the R259H mutation was detected in Chinese people.
基金This work was supported by the Fudan University Nursing Research Foundation(Fund#FNF201706).
文摘Objectives:The integrated model of frailty shifted the physiologically-dominated view to an integrated and multidisciplinary view of frailty and adopted an integrated and holistic approach to the complex challenges facing frail older adults and their caregivers.There is an urgent need for researchers to identify multi-faceted frailty among older adults and to explore contributors to it.We assessed the level of frailty among older residents in long-term care(LTC)facilities in Shanghai,China and explored risk factors of multiple dimensions of frailty among older residents.Methods:It is a cross-sectional descriptive study.We interviewed 218 older residents from nine LTC facilities.We used Tilburg Frailty Indicator to assess older residents’physiological,psychological,and social frailty.The Geriatric Depression Scale,Mini-Mental state examination,Athens Insomnia Scale,and Activity of Daily Living(ADL)Scale were used to assess their depressive symptoms,cognitive function,sleep quality and ADL abilities,respectively.Results:Older residents have a moderate level of frailty in physiological,psychological and social domains.There is a high correlation among dimensions of frailty.Older residents’depressive symptoms have remained positively associated with their overall and each domain of frailty.Older residents’actual activity participation and exercise in LTC facilities are negatively associated with older residents’overall,physiological and social frailty.Conclusions:Frailty is a multi-dimensional concept and is prevalent among older residents in LTC facilities.It is important to take an integrated view on frailty and carry out appropriate and comprehensive interventions to prevent adverse outcomes and provide holistic care for frail older residents in LTC facilities.There is an urgent need to improve the quality and expand the number of activities offered within LTC facilities that would engage older residents in a person-centered way,despite their age,ADL abilities,frailty and functional limitations.
文摘Reading strategies are different from identifying the words, but a kind of metacognitive activity based on the monitoring mode. This paper will explore Chinese English learners＇ text reading strategies according to the cognitive process of the reading, hoping for providing some suggestions and references for Chinese college students to learn English well and improve the text reading ability.
基金funded by earthquake security infrastructure of Tianjin 11th "Five-year Plan" (Tianjin Development and Reforming Office[2009]-1230),the Spark Program of Earthquake Sciences(Grant No.XH13002)
文摘Based on faults surveying and research data in the Tianjin offshore areas,through studying tectonic structure,Quaternary activity,deep structure,stress and strain fields and seismicity in the Tianjin offshore areas,the activity and tectonic features of the faults are determined synthetically.Using seismo-geological data,and the historical and modern seismicity data,the frequency-magnitude relationship model normalized by 500a is established and based on the relationship between the upper limit of maximum magnitude M u and a t/b,the maximum magnitudes of the sea section of the Haihe river fault and the Haiyi fault are calculated.Then Poisson probability model is adopted and the quantitative parameters,such as the maximum magnitude,occurrence probability,recurrence cycle of the faults in the south Tianjin offshore areas in the coming 50～200a,are calculated.
基金supported by the National Natural Science Foundation of China (Grant No. 50971030)the National Basic Research Program of China (Grant Nos. 2009GB109004 and 2011GB108004)
文摘China Low Activation Martensitic （CLAM） steel is being studied to develop the structural materials for a fusion reactor, which has been designed based on the well-known 9Crl.5WVTa steel. The effect of tempering temperature on hardness and micro- structure of CLAM steel was studied. The strength of CLAM steel increased by adding silicon, and the ductility remained con- stant. Conversely, while CLAM steel maintained good ductility with the addition of yttrium, its tensile strengths were greatly degraded. Behaviors under electron irradiation of CLAM steel were examined using the high voltage electron microscope. Electron irradiation at 450℃ formed many voids in CLAM steel with basic composition, whereas CLAM with silicon steel did not change the microstructure significantly.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41201502 and 31170305)China Postdoctoral Science Special Foundation (Grant No. 2012T50508)China Postdoctoral Science Foundation (Grant No. 2011M501010)
文摘Human activities are strongly modifying the global nitrogen (N) cycle through increasing input, N species diversity, and pool size of industrial reactive N (Nr). However, the fluxes, fates and environmental consequences of industrial Nr (excluding synthesized N fertilizer) remain poorly understood and quantified. We report here that industrial Nr flux has increased 13.4-fold over the past 30 years in China, reaching 3.7Tg N (1 Tg=10 12g) in 2008, accounting for over 50% of China's food Nr flux. Socioeconomic development (per capita GDP, urbanization and household size) significantly drives the growth of industrial Nr fluxes. This leads to "hotspots" of industrial Nr, mainly in relatively developed Eastern China. Industrial Nr loss rate during production is only 5%, much lower than that of cropland (50%) and livestock (80%). However, industrial Nr loss is point source pollution, and Nr release in concentrated doses produces serious risk in small regions. The contribution of structural N to total industrial Nr with a lifespan longer than one year (e.g., synthetic fiber, plastic) increased from 20% in 1980 to 70% in 2008. There was about 2.6 Tg N structural industrial Nr accumulated in human settlements in 2008, which could be one ex- planation of an unknown Nr sink of anthropogenic Nr input (mainly Haber-Bosch N fixation). Legacy effects caused by structural N accumulation have long-term consequences for environmental and human health, although structural N delays Nr release and reduces short-term Nr pollution. Industrial Nr use generates new features of modern global N biogeochemistry, such as increasing Nr species diversity, reducing Nr turnover rate. Future dynamics simulation of the earth system should involve industrial Nr. Explicit consideration and accounting of the fluxes and environmental consequences of industrial Nr would provide decision-makers a novel view of regional sustainable development.
