丛枝-泡囊菌根真菌——中国球囊霉在离体条件下的生长和产孢

丛枝-泡囊菌根(Vesicular-arbuscular mycorrhizae,VAM)真菌在离开寄主组织条件下的纯培养一直没有得到很好的解决,致使这类真菌的分类、遗传和生理研究以及它们在生产上的应用都有很大的局限性。由于VAM真菌在生产上应用的潜能。

用三叶草离体根建立VA菌根

红三叶草(Trifolium pratense L.)离体根在改良的White培养基上能连续继代培养。将发芽的中国球囊霉(Glomus sinensis Peng & Shen)孢子接种三叶草离体根,在以 CaHPO_4为磷源的 White 培养基上培养,能稳定地形成泡囊-丛枝状内生菌根(Vesicular-ArbuscularMycorrhizae,VAM)。真菌在侵染前,菌丝在根分泌物的刺激下常进一步伸长,当菌丝接触到根的适当部位后即开始侵染,侵入前常首先形成附着胞,并通过附着胞进一步向根内侵染,形成具有根内菌丝、丛枝和不明显泡囊的内生菌根。随后产生根外菌丝,并产生少量薄壁的'无性孢子'。这些'无性孢子'不能发育成正常的厚垣孢子。本文讨论了这一技术在研究 VA 菌根中的作用和意义。

八卦新闻

突发疾病尼克斯成绩的下跌一直都是困扰主帅布朗的核心问题,如今年事已高的布朗还要亲临激烈竞争的赛场,让不少人为之身体而担忧。这不,尼克斯做客孟菲斯时,布朗突感胸口疼痛,之后他立即被送往当地的一家医院进行检查,但结果却没有想像中糟糕,只是虚惊一场。

Contentious Petroleum Geochemical Issues in China’s Sedimentary Basins

Petroleum geochemistry contributes to exploration successes by providing key constraints for geological models and critical input to exploration scenarios. One of the most important tasks in a typical exploration program is to identify the most effective source intervals or kitchens in a basin, through oil-source correlation. The results of correlation are valid only if the geochemical parameters used address adequately the genetic characteristics of the source rocks as well as the mass transport and mixing processes of hydrocarbon fluids occurring in the carrier beds and reservoirs. This manuscript discusses four of the major contentious petroleum geochemical issues in China’s sedimentary basins. It is suggested that marine incursions played a significant role in the formation of prolific petroleum source rocks in the gigantic, dominantly freshwater, Songliao Basin. Several models are proposed to account for the occurrence of immature oils in the Cathaysian rift system including the Bohai Bay Basin, thus immature source rocks are considered a mere minor contributor to the known economic immature oil resources. Both geological and geochemical evidence are reviewed to refute a dominantly coaly source for the petroleum discovered in the Turpan Basin. Results of case studies are presented to demonstrate the importance of recognizing petroleum fluid mixing to solve the oil-source correlation issues in the structurally complex Tarim Basin. In addressing the fundamental assumptions and potential flaws of the molecular geochemical parameters commonly used for oil-source correlation, the need of a mass fraction approach is proposed to deal with such contentious issues as marine versus lacustrine, coal versus lacustrine, and mature versus immature oils.

The Two Main Mechanisms of Glacier Lake Outburst Flood in Tibet,China

With the global warming,the disasters of Glacier Lake Outburst Flood(GLOF) have taken place frequently in Tibet in recent years and attracted more and more attention.A systematic survey was conducted on the 19 GLOFs in Tibet to study their two main mechanisms.Investigations indicated that all the events occurred in end-moraine lakes,and the outburst occurred partially and instantly.And the breach had the shape of an arc or a trapezoid in overflow outburst and its top width was 3-5 times more than the height.The two main mechanisms of GLOFs in Tibetan end-moraine Lake were overflow and piping,and the overflow mechanism caused by iceberg collapse was dominated in most cases.A formula was proposed to calculate the critical thickness of iceberg tongue that determines the collapse.Granular analysis of the moraine materials revealed that seepage deformation is crucial in the outburst process.Finally,we conducted a case study of the Guangxiecuo Lake to show its possible process of outburst and estimated the peak discharge of the resulted flood.

Autumn Photoproduction of Carbon Monoxide in Jiaozhou Bay, China

Carbon monoxide(CO) plays a significant role in global warming and atmospheric chemistry. Global oceans are net natural sources of atmospheric CO. CO at surface ocean is primarily produced from the photochemical degradation of chromophoric dissolved organic matter(CDOM). In this study, the effects of photobleaching, temperature and the origin(terrestrial or marine) of CDOM on the apparent quantum yields(AQY) of CO were studied for seawater samples collected from Jiaozhou Bay. Our results demonstrat that photobleaching, temperature and the origin of CDOM strongly affected the efficiency of CO photoproduction. The concentration, absorbance and fluorescence of CDOM exponentially decreased with increasing light dose. Terrestrial riverine organic matter could be more prone to photodegradation than the marine algae-derived one. The relationships between CO AQY and the dissolved organic carbon-specific absorption coefficient at 254 nm for the photobleaching study were nonlinear, whereas those of the original samples were strongly linear. This suggests that: 1) terrestrial riverine CDOM was more efficient than marine algae-derived CDOM for CO photoproduction; 2) aromatic and olefinic moieties of the CDOM pool were affected more strongly by degradation processes than by aliphatic ones. Water temperature and the origin of CDOM strongly affected the efficiency of CO photoproduction. The photoproduction rate of CO in autumn was estimated to be 31.98 μmol m-2 d-1 and the total DOC photomineralization was equivalent to 3.25%- 6.35% of primary production in Jiaozhou Bay. Our results indicate that CO photochemistry in coastal areas is important for oceanic carbon cycle.

Early warning for geo-hazards based on the weather condition in China

According to national early warning practice for geo-hazards from 2003 to 2005,it is systematically concluded that the basic characteristics of geo-hazards,early warning method and forecast result based on the geological maps of China in a scale 1∶6 000 000.With the contrast of different characters between sustained rainfall and typhoon rainfall inducing geo-hazards,the disaster reduction result and some problems are preliminarily analyzed.Some basic recognition is that early warning to geo-hazards is feasible,national scale forecast is only to call attention,but can't immediately be used to disaster reduction decision-making.And,the future direction is to build a united disaster reduction framework of early warning system including national,provincial and county levels based on weather factors in different scale of area.

Late Quaternary Strata and Carbon Burial Records in the Yellow River Delta, China

Sediment carbon sequestration plays an essential role in mitigating atmospheric CO2 increases and the subsequently global greenhouse effect. To clarify the late Quaternary strata and carbon burial records in YeUow River delta （YRD）, detailed analysis of benthic foraminifera, total carbon （TC）, organic carbon （Corg）, sedimentary characteristics and moisture contents of sediments, was performed on core ZK3, 30.3 m in length and obtained from YRD in 2007. Eight depositional units （designated U1-U8 in ascending order） were identified. A comprehensive analysis method of historical geography and sedimentary geology was used to de- termine the precise depositional ages of the modem Yellow River delta （MYRD）, from which pre-MYRD ages were deduced. The results indicates that the maximum burial rates of TC, inorganic carbon （IC） and Corg occurred in the delta front （U5）, and the mini- mum in the shallow sea （U3）. Remarkable high sedimentation rates in the MYRD are responsible for burial efficiency of carbon, with an average rate of Corg burial reaching 2087±251 g（m2yr）-1, and that of IC reaching 13741±808g（m2yr）-1, which are much higher than those of other regions with high contents of Corg. Therefore, YRD has a significant burial efficiency for carbon sequestration.

The Main Content,Technical Support and Enforcement Strategy of Digital Agriculture

This paper deals with the content,purpose and significance of digital agriculture,and gives a brief introduction to digital earth,digital China and digital agriculture abroad.The main content of digital agriculture includes the construction of database,Metadata standard,monitoring system,forecasting and decision_making system,information_releasing system.The main technical supports of digital agriculture are 3S technology,Metadata management technology,fast_handling technology of remote sensing,the technology of computer wide_band network and virtual reality,intelligent agriculture machinery and field real_time information collecting technology.The advantages of implementing digital agriculture in China and the problems worthy of attention are pointed out as well.

Evaluation of global high-resolution reanalysis products based on the Chinese global oceanography forecasting system

The global high-resolution marine reanalysis products that were independently developed by the National Marine Environmental Forecasting Center based on the Chinese Global Oceanography Forecasting System(CGOFS),are evaluated by comparing their climatologies with internationally recognized data from WOA(Word Ocean Atlas),SODA(Simple Ocean Data Assimilation),AVISO(Archiving,Validation,and Interpretation of Satellite Oceanographic Data),and C-GLORS(Global Ocean Reanalysis System).The results show that the SST RMSEs of CGOFS and SODA against WOA are 0.51℃ and 0.43℃ respectively;and in the North Pacific,the SST of CGOGS is closer to that of WOA than SODA.The SSS RMSEs of CGOFS and SODA compared with WOA are 0.48 PSU and 0.40 PSU,respectively.CGOFS can reproduce the main large-scale ocean circulation globally,and obtain a similar vertical structure of the Equatorial Undercurrent as SODA.The RMSE of the CGOFS global sea-level anomaly against AVISO is 0.018 m.The monthly averaged sea-ice extents are between those of SODA and C-GLORS in each month;the growth and ablation characteristics of the ice volume are consistent with SODA and C-GLORS;but the ice volume of CGOFS is greater than that of SODA and C-GLORS.In general,the climatology of the CGOFS global high-resolution reanalysis products are basically consistent with similar international products,and can thus provide reliable data for the improvement of marine science and technology in China.

Behavior of rare earth elements in granitic profiles, eastern Tibetan Plateau, China

Rare earth elements(REEs) can record geologic and geochemical processes. We studied two granitic regolith profiles from different climatic zones in eastern Tibetan Plateau and found that(1)∑ RREEs ranged from119.65 to 275.33 mg/kg in profile ND and5.11–474.55 mg/kg in profile GTC, with average values of205.79 and 161 mg/kg, respectively. ∑RREEs was higher in accumulation horizon and semi-regolith;(2) Influenced by climate, the fractionation of light and heavy REEs(LREEs and HREEs) varied during weathering. The ratio of LREEs/HREEs in pedosphere was higher than semi-regolith in tropical profile;(3) A negative Eu anomaly in both profiles was the result of bedrock weathering. A positive Ce anomaly was observed in all layers of profile ND, and only in the upper 100 cm of profile GTC. This indicates that redox conditions along the regolith profile varied considerably with climate.(4) Normalized by chondrite,LREEs accumulated much more than HREEs; REE distribution curves were right-leaning with a V-type Eu anomaly in both profiles.

Changing Connectivities of Chinese Cities in the World City Network,2010–2016

Against the backdrop of the sizable economic growth of China in recent years, this paper uses the most recent data gathering of the Globalization and World Cities(Ga WC) research network to update and supplement earlier research on the shifting global connectivity of Chinese cities. The update consists of an evaluation of the connectivity of Chinese cities in 2016, the supplement of an analysis of the changing position of Chinese cities in the world city network between 2010 and 2016. To this end, we build on a specification of the world city network as an ‘interlocking network' in which producer services firms play the crucial role in city network formation. Information about the presence of leading producer services firms in cities in 2010 and 2016 is used as the input to a bipartite network projection algorithm in order to measure cities' network connectivity. The first set of results discusses the geographies of urban connectivity in the world city network in 2016. The second set of results discusses standardized measures of change to reveal the major dimensions of the transformations between 2010 and 2016. We find that, with the exception of Hong Kong, Macao and Kaohsiung of Taiwan, all Chinese cities record connectivity gains. This wholesale rise in connectivity is nonetheless geographically uneven, with above all Beijing, Chengdu/Chongqing and Changsha/Wuhan becoming more connected. We conclude that the wholesale rise of Chinese cities in the world city network and their changing trajectories in the post-crisis era are embedded in shifting external and internal political economies.

World Cities and International Organizations: Political Global-city Status of Chinese Cities

In this study, the authors use a political perspective to explore the relative global status of cities in China. Two questions are addressed. Firstly, by using international organizations as the subjects and quantitative analysis of the spatial distribution of their offices, the overall position of Chinese cities in the global distribution of international organizations is estimated. The results show that 22 Chi- nese cities are involved in international political affairs. Beijing, Shanghai and Guangzhou dominate these activities and have high po- litical global-city status. Secondly, through qualitative analysis of Guangzhou City, the authors explore how its status as a center for intemational organizations has been shaped in recent years. The locational decision-making process is analyzed and the factors are demonstrated at both national and city scales. The definition of international organizations as used in this study refers not only to those supranational bodies that have the ability to manage relations across states （such as the European Union）, but also those in- ter-governmental organizations （IGOs）, international non-governmental organizations （INGOs） and diplomatic representatives and delegations that today also manage and regulate relations between and among states.

Research on the Characteristics of Large Earthquake Activity on the Active Tectonic Boundaries on the Chinese Mainland

Based on the research and the division of the active tectonic blocks and their boundaries on the Chinese mainland, the feature of the large earthquake activities on the 24 boundaries between the 6 active tectonic block regions (grade Ⅰ) and the 22 active tectonic blocks (grade Ⅱ) are studied. The seismicity levels on the active tectonic block boundaries are discussed considering the large earthquake frequency and the released strain energy in unit distance and time. The theoretic maximal magnitude and the recurrence period of each boundary are then calculated from the G-R relation. By comparing this with the actual earthquake records, it is found that the intensities of the earthquake deduced from the seismic activity parameter (a/b) on the main active boundaries on the Chinese mainland are consistent with that of the natural earthquakes. Meanwhile, an inverse relation is found between the recurrence periods of large earthquakes and the tectonic motion rate on the boundaries. These results show that the a, b values of each boundary obtained in this paper are valuable. In addition, the present seismic activities and hazards of these boundaries are also probed into with the historical data and their elapsed time on each boundary based on the hypothesis that the large earthquakes satisfy Poisson distribution.

China's Global Energy Strategy: from Energy Strength to Energy Power

Since the founding of the People's Republic of China in 1949, China's global energy strategy has experienced three stages: "infancy", "growth" and "maturity". Chinese foreign energy cooperation has also gone through three periods chronologically, which include(1) the period when China got rid of its high dependence on oil imports from the Soviet Union, achieved self-reliance and even became a net exporter;(2) the period when China first focused on "bringing in" and then turned itself into a net importer of crude oil and refined oil; and(3) the period when China "went global" to engage in energy cooperation in diverse areas, gradually shifting into a leading role before launching the Belt and Road Initiative, which marked the full establishment of the Chinese global energy strategy. The objective of the Chinese global energy strategy has gradually changed from merely pursuing "energy strength" to transforming "energy strength" into "energy power"; the mission is mainly to "promote energy cooperation and energy revolution"; to implement this mission, China will provide international public goods to global and regional energy governance through energy diplomacy and services; and the areas covered by the strategy are countries and regions along the "Silk Road Economic Belt".

Impact of Climatic Change on Agricultural Production and Response Strategies in China

A number of studies indicate that global climate warming has been increasing, especially in recent decades. Climate warming greatly affects global agro-production and food security-- becoming a hotspot of global environmental change. This paper proposes a structural and orientational framework for scientifically addressing climatic change impact on agroroduction. Through literature reviews and comparative studies, the paper systematically summarizes influencing mechanisms and impact of climate warming on such agro-production factors as light, temperature, soil quality and water environment. The impact of climate warm- ing on cultivation regions, cropping systems, crop pests, agro- production capacity, agro-economy and farm management is analyzed. Then, suitable climate-adapting agro-development strategies are put forward for different regions in China. The strategies are carefully selected from a repository of international tested climatic change countermeasures in agriculture at national or district level.

A New Species of the Genus Chinogastrura (Collembola: Hypogastruridae) from China

The present paper dealed with the genus Chinogastrura and a new species, Chinogastrura quinidentis, sp. nov. from Jiangsu Province, China. New species is similar to the species Ch. duplicispinosa （Yosii）, 1954. However, it is separable from the latter by the peculiar tenaculum, dorsal setae on manubrium and other features.

Formation and evolution of the modern warm current system in the East China Sea and the Yellow Sea since the last deglaciation

To reconstruct the formation and evolution process of the warm current system within the East China Sea （ECS） and the Yellow Sea （YS） since the last deglaciation, the paleoceangraphic records in core DGKS9603, core CSH1 and core YSDP102, which were retrieved from the mainstream of the Kuroshio Current （KC）, the edge of the modern Tsushima Warm Current （TWC） and muddy region under cold waters accreted with the Yellow Sea Warm Current （YSWC） respectively, were synthetically analyzed. The results indicate that the formation and evolution of the modern warm current system in the ECS and the YS has been accompanied by the development of the KC and impulse rising of the sea level since the last deglaciation. The influence of the KC on the Okinawa Trough had enhanced since 16 cal kyr BP, and synchronously the modern TWC began to develop with the rising of sea level and finally formed at about 8.5 cal kyr BP. The KC had experienced two weakening process during the Heinrich event 1 and the Younger Drays event from 16 to 8.5 cal kyr BP. The period of 7-6 cal kyr BP was the strongest stage of the KC and the TWC since the last deglaciation. The YSWC has appeared at about 6.4 cal kyr BE Thus the warm current system of the ECS and the YS has ultimately formed. The weakness of the KC, indicated by the occurrence of Pulleniatina minimum event （PME） during the period from 5.3 to 2.8 cal kyr BE caused the main stream of the TWC to shift eastward to the Pacific Ocean around about 3 cal kyr BE The process resulted in the intruding of continent shelf cold water mass with rich nutrients. Synchronously, the strength of the YSWC was relatively weak and the related cold water body was active at the early-mid stage of its appearance against the PME background, which resulted in the quick formation of muddy deposit system in the southeastern YS. The strength of the warm current system in the ECS and the YS has enhanced evidently, and approached to the modern condition gradually since 3 cal kyr BE

SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY

Serum immunoglobulin from the mandarin fish, or the so called Chinese perch, Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM of S. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains of S. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.

Identification of Ice Elevation Change of the Shuiguan River No.4 Glacier in the Qilian Mountains,China

GPS measurement,an effective method for surveying glacier surface topography,has been applied in some glaciers for many years.The Shuiguan River No.4 glacier,a small glacier with its area of 1.84 km2 in 1972,located in the east of the Qilian Mountains,China,was selected to study its ice elevation change using GPS measurement in 2007.This study was conducted on the ablation area with GPS-measured area 0.5 km2.The ice elevation change of the glacier was obtained by comparing the DEM obtained by a 1:50 000 topographic map made in 1972 with the DEM by GPS-measured data acquired in 2007.The differences of the two DEMs showed the thinning condition of the glacier was apparent.The mean thinning was 15±8 m with the mean thinning rate of 0.42±0.22 m a-1 for 1972-2007 in the measurement area,which equaled 0.38±0.20 m yr-1 in water equivalent(w.e.).The prominent thinning occurred on the south part of the glacier,which was the area near the glacier terminus with the maximum thinning of 41±8 m.Assuming the thinning value of 15±8 m for the glacier area below 4640 m a.s.l.,the wasting ice mass was calculated to be 6.4±3.2×10-3 km3 for 1972-2007,corresponding to 5.7±2.8×10-3 km3 w.e.,which meant that the montane runoff released by the glacier was at least 5.7±2.8×106 m3 between 1972-2007.