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中国红树类海桑属植物DNA条形码研究 被引量:3
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作者 孙稚颖 李强 +2 位作者 田晓萌 郭庆梅 邵长伦 《世界科学技术-中医药现代化》 CSCD 北大核心 2021年第6期2036-2042,共7页
目的对中国的6种红树类海桑属植物进行DNA条形码研究,为该属植物鉴定、资源保护、合理开发等提供参考依据。方法以核基因ITS2片段及叶绿体基因片段psbA-trnH作为DNA条形码,对分布在中国的海桑属植物进行基因组DNA提取、PCR扩增和双向测... 目的对中国的6种红树类海桑属植物进行DNA条形码研究,为该属植物鉴定、资源保护、合理开发等提供参考依据。方法以核基因ITS2片段及叶绿体基因片段psbA-trnH作为DNA条形码,对分布在中国的海桑属植物进行基因组DNA提取、PCR扩增和双向测序。所得序列经CodonCode Aligner拼接获得叶绿体psbA-trnH基因间区序列和核ITS2序列,用软件MEGA6.0进行相关数据分析,构建NJ(邻接)树。结果我国6种红树类海桑属植物的ITS2序列间存在明显差异,psbA-trnH序列间也存在一定的碱基差异,表明6种海桑属植物具有其各自独特的DNA条形码,可以相互区别,同时基于ITS2序列及psbA-trnH序列构建的邻接(NJ)树可以看出6种海桑属植物具有或近或远的亲缘关系。结论核ITS2和叶绿体psbA-trnH序列作为DNA条形码可以有效地区分和鉴别我国6种红树类海桑属植物,为后续药用资源开发等相关研究提供了参考。 展开更多
关键词 中国红树类 海桑属植物 ITS2 PSBA-TRNH DNA条形码
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Mangrove Flora of the World 被引量:13
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作者 王伯荪 梁士楚 +1 位作者 张炜银 昝启杰 《Acta Botanica Sinica》 CSCD 2003年第6期644-653,共10页
The species composition and characteristics of mangrove flora in the world were reviewed and discussed. The results suggested that the world's mangrove plants have 84 species (including 12 varieties) in 24 genera ... The species composition and characteristics of mangrove flora in the world were reviewed and discussed. The results suggested that the world's mangrove plants have 84 species (including 12 varieties) in 24 genera and 16 families. Of which, true mangrove plants have 70 species (including 12 varieties) in 16 genera and 11 families, and semi-mangrove plants 14 species in eight genera and five families. The Eastern Group has 74 species (including 12 varieties) in 18 genera and 14 families, characterized by the genera Aegiceras, Osbornia, Aegialitis, Bruguiera, Ceriops, Kandelia, Scyphiphora and Nypa etc. The Western Group has only 10 species in six genera and five families, characterized by the endemic one-species family, Pelliceraceae, and the genus Laguncularia. The mangrove flora of China is composed of 26 species (including one variety) in 15 genera and 12 families, four of which are endemic. Hainan is most rich in mangrove species, making up about 96.2% of the Chinese total; Guangdong ranks second, making up about 42.3%. It has been demonstrated that Rhizophora stylosa was mistaken for R. mucronata in Taiwan by previous authors. 展开更多
关键词 MANGROVE FLORA floral composition Rhizophora mucronata
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