AIM： To study the loss of heterozygosity （LOH） on 12p12-13 in Chinese colon carcinoma patients.METHODS： DNA was extracted from 10 specimens of cancer tissue, 10 specimens of adjacent tissue and 10 specimens of nor...AIM： To study the loss of heterozygosity （LOH） on 12p12-13 in Chinese colon carcinoma patients.METHODS： DNA was extracted from 10 specimens of cancer tissue, 10 specimens of adjacent tissue and 10 specimens of normal tissue, respectively. LOH of Kras2 gene was analyzed by polymerase chain reaction （PCR） and denaturing polyacrylamide gel electrophoresis using 11 microsatellite markers on 12p-12-13.RESULTS： LOH of Kras gene was detected at least on one marker of 12p-12-13 in 30% （3/10） of adjacent tissue specimens. The highest frequency of LOH was identified on D12S1034 in 28.57% （2]7） of adjacent tissue specimens. LOH was detected at least on one marker of 12p12-13 in 60% （6/10） of carcinoma tissue specimens, the most frequent LOH was found on D12S1034 and D12S1591 in 42.86% （3/7） of carcinoma tissue specimens. LOH was detected in 30% （3/10） of carcinoma tissue specimens, 30% （3/10） of adjacent tissue specimens, and no signal in 1% （1/0） carcinoma tissue specimen. The occurrence of LOH did not correlate with sex, age, tumor size and lymph node metastasis.CONCLUSION： Genomic instability may occur on 12p-12-13 of Kras2 gene in the development and progression of colon carcinoma. The high LOH of Kras2 gene may directly influence the transcription and translation of wild type Kras2 gene.展开更多
The Asar va Ahyā’is a manual of agricultural production written in the early fourteenth century by the famous Iranian vizier and scholar Rashīd al-Dīn.Only chapter 6 to chapter 13 of the original work survive,deal...The Asar va Ahyā’is a manual of agricultural production written in the early fourteenth century by the famous Iranian vizier and scholar Rashīd al-Dīn.Only chapter 6 to chapter 13 of the original work survive,dealing with agriculture and horticulture,and nearly a third of the pages of which are devoted to introducing Chinese plants and related knowledge.In addition to introducing botanical knowledge,the book also covered information on all the aspects of the Yuan dynasty(1271–1368),including the taxation system,monetary system,military system,economy and trade,manufacturing techniques,ethnic customs,food culture,and other fields,providing important materials for research on the dissemination of species,exchange of technologies,communication of ethnic groups,and integration of cultures between China and the world during the thirteenth and fourteenth centuries.Rashīd al-Dīn,the book’s author,took advantage of his identities as a statesman and a businessman to collect rich information about plants all over the world.Motivated by his specific interest in Chinese culture,he included a huge amount of Chinese information in the book.Meanwhile,records in the Asar va Asyā’reflect the author’s practice of researching,planting,and cultivating plants,making the book not only a compilation of agricultural knowledge,but also a report that recorded the author’s agricultural practice.展开更多
基金Supported by the National Natural Science Foundation of China,No.30200326
文摘AIM： To study the loss of heterozygosity （LOH） on 12p12-13 in Chinese colon carcinoma patients.METHODS： DNA was extracted from 10 specimens of cancer tissue, 10 specimens of adjacent tissue and 10 specimens of normal tissue, respectively. LOH of Kras2 gene was analyzed by polymerase chain reaction （PCR） and denaturing polyacrylamide gel electrophoresis using 11 microsatellite markers on 12p-12-13.RESULTS： LOH of Kras gene was detected at least on one marker of 12p-12-13 in 30% （3/10） of adjacent tissue specimens. The highest frequency of LOH was identified on D12S1034 in 28.57% （2]7） of adjacent tissue specimens. LOH was detected at least on one marker of 12p12-13 in 60% （6/10） of carcinoma tissue specimens, the most frequent LOH was found on D12S1034 and D12S1591 in 42.86% （3/7） of carcinoma tissue specimens. LOH was detected in 30% （3/10） of carcinoma tissue specimens, 30% （3/10） of adjacent tissue specimens, and no signal in 1% （1/0） carcinoma tissue specimen. The occurrence of LOH did not correlate with sex, age, tumor size and lymph node metastasis.CONCLUSION： Genomic instability may occur on 12p-12-13 of Kras2 gene in the development and progression of colon carcinoma. The high LOH of Kras2 gene may directly influence the transcription and translation of wild type Kras2 gene.
文摘The Asar va Ahyā’is a manual of agricultural production written in the early fourteenth century by the famous Iranian vizier and scholar Rashīd al-Dīn.Only chapter 6 to chapter 13 of the original work survive,dealing with agriculture and horticulture,and nearly a third of the pages of which are devoted to introducing Chinese plants and related knowledge.In addition to introducing botanical knowledge,the book also covered information on all the aspects of the Yuan dynasty(1271–1368),including the taxation system,monetary system,military system,economy and trade,manufacturing techniques,ethnic customs,food culture,and other fields,providing important materials for research on the dissemination of species,exchange of technologies,communication of ethnic groups,and integration of cultures between China and the world during the thirteenth and fourteenth centuries.Rashīd al-Dīn,the book’s author,took advantage of his identities as a statesman and a businessman to collect rich information about plants all over the world.Motivated by his specific interest in Chinese culture,he included a huge amount of Chinese information in the book.Meanwhile,records in the Asar va Asyā’reflect the author’s practice of researching,planting,and cultivating plants,making the book not only a compilation of agricultural knowledge,but also a report that recorded the author’s agricultural practice.
