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六组点堆中子动力学方程组的同伦分析解 被引量:2
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作者 朱倩 商学利 陈文振 《物理学报》 SCIE EI CAS CSCD 北大核心 2012年第7期1-5,共5页
鉴于目前六组点堆中子动力学方程仍然无法获得解析解,本文尝试将同伦分析方法应用于六组缓发中子动力方程组的求解,获得了它的级数解析解,并对级数解析解算法的有效性进行了检验.结果表明,该级数解析解算法从计算时间和精度上都能达到... 鉴于目前六组点堆中子动力学方程仍然无法获得解析解,本文尝试将同伦分析方法应用于六组缓发中子动力方程组的求解,获得了它的级数解析解,并对级数解析解算法的有效性进行了检验.结果表明,该级数解析解算法从计算时间和精度上都能达到工程应用的要求,可适宜于反应堆中子动力学控制的设计分析和仿真计算. 展开更多
关键词 同伦分析法 中子动力学 点堆方程 缓发中子
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宇宙生命的狂想曲
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作者 刘八一 李晓勇 《现代物业(中旬刊)》 2010年第10期103-103,99,共2页
宇宙基本运动体的运动形成的生命能势的产生;天体、天体系的形成;天体系生命环境的形成,地球上有机生命的形成。
关键词 中子组 能势态 势能平衡
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Overexpression of connective tissue growth factor WISP-1 in Chinese primary rectal cancer patients 被引量:11
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作者 Chao Tian Zong-Guang Zhou +7 位作者 Wen-Jian Meng Xiao-Feng Sun Yong-Yang Yu Li Li Hong-Zhi Luo, Lie Yang Bin Zhou Jun Gu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第28期3878-3882,共5页
AIM: To clarify the expression change of Wnt-induced secreted protein-1 (WISP-l) in human rectal cancer and to determine whether it is correlated with invasion and metastasis of human rectal cancer.METHODS: Eighty... AIM: To clarify the expression change of Wnt-induced secreted protein-1 (WISP-l) in human rectal cancer and to determine whether it is correlated with invasion and metastasis of human rectal cancer.METHODS: Eighty-six paired samples of rectal cancer and surgically resected distant normal rectal tissue were collected and allocated into cancer group and control group respectively. WISP-1 mRNA was detected by relative quantitative real-time RT-PCR and WISP-1 protein was examined by immunohistochemical staining.RESULTS: WISP-1 gene overexpression was found in 65% (56/86) primary rectal cancers, 2-30 times that of the level in normal matched rectal tissues (P = 0.001). The mRNA expression level was correlated with Duke's staging, histological differentiation grade and lymph node status. The WISP-1 protein expression was in accordance with mRNA expression level. The positive degree of immunohistochemical staining in the cancer group (1.40±0.35) was different from that in control group (1.04± 0.08, P 〈 0.001). Moreover, in cancer group the positive staining degree in high-level mRNA cancers (1.46 ±0.37, n = 56) was higher than that in low-level mRNA (1.28 ± 0.28, n = 30, P = 0.018).CONCLUSION: Aberrant levels of WISP-1 expression may play a role in rectal tumorigenesis. WISP-1 may be used as a specific clinical diagnosis and prognosis marker in rectal cancer. 展开更多
关键词 Wnt-induced secreted protein-1 Rectal cancer Real time RT-PCR Immunohistochemicy
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Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca^(2+)-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats 被引量:20
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作者 Ping Xue Li-Hui Deng +5 位作者 Zhao-Da Zhang Xiao-Nan Yang Qing Xia Da-Kai Xiang Lei Huang Mei-Hua Wan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第15期2343-2348,共6页
AIM: To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. METHODS: Thirty Sprague-Dawl... AIM: To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. METHODS: Thirty Sprague-Dawley (SD) rats were randomized into control group, AP group and CQCQD group (n = 3 × 10). The rats in the CQCQD group were intragastrically administered with CQCQD (10 mL/kg every 2 h) after induction of AP by intraperitoneal injection of caerulein (50 μg/kg.h × 5) within 4 h. At 6 h after the induction of AP model, pancreatic tissues were collected for the pathological observation, mRNA extraction for determination of SERCA1 and SERCA2 mRNA expression or pancreatic acinar cell isolation for measurement of fluorescence intensity (FI) of intracellular calcium ion concentration [Ca2+]i. RESULTS: There was no expression of pancreatic SERCA1 mRNA in the control group and the AP group. The expression of pancreatic SERCA2 mRNA in the AP group was down-regulated (expression ratio = 0.536; P = 0.001) compared with the control group, while that in the CQCQD group was up-regulated (expression ratio= 2.00; P = 0.012) compared with AP group. The FI of intracellular [Ca2+] of pancreatic acinar cells in the AP group (138.2 ± 23.1) was higher than the C group (111.0 ± 18.4) and the CQCQD group (118.7 ± 15.2 ) (P < 0.05) and the pancreatic pathological score in the CQCQD group was lower than that in the AP group (5.7 ± 1.9 vs 9.2 ± 2.7, P < 0.05).CONCLUSION: CQCQD can up-regulate the expression of SERCA2 mRNA of pancreatic tissues, reduce intracellular calcium overload and relieve pancreatic tissue lesions. 展开更多
关键词 Chaiqinchengqi decoction PANCREATITIS Calciumoverload Sarco/endoplasmic reticulum Ca^2+-ATPase
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Tumor metastasis and the reciprocal regulation of heparanase gene expression by nuclear factor kappa B in human gastric carcinoma tissue 被引量:18
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作者 Hou-JunCao YongFang XingZhang Wen-JunChen Wen-PengZhou HongWang Lin-BoWang Jin-MinWu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第6期903-907,共5页
AIM: To investigate whether NF-kB is activated in human gastric carcinoma tissues and, if so, to study whether there is any correlation between NF-kB activity and heparanase expression in gastric carcinoma. METHODS: N... AIM: To investigate whether NF-kB is activated in human gastric carcinoma tissues and, if so, to study whether there is any correlation between NF-kB activity and heparanase expression in gastric carcinoma. METHODS: NF-kB activation was assayed by immunohistochemical staining in formalin-fixed, paraffin-embedded specimens from 45 gastric carcinoma patients. Electrophoretic mobility shift assay (EMSA) method was used for nuclear protein from these fresh tissue specimens. Heparanase gene expression was quantified using quantitative RT-PCR. RESULTS: The nuclear translocation of RelA (marker of NF-kB activation) was significantly higher in tumor cells compared to adjacent and normal epithelial cells [(41.3±3.52)% vs (0.38±0.22) %, t=10.993, P= 0.000<0.05; (41.3±3.52)% vs(0±0.31)%, t=11.484, P= 0.000<0.05]. NF-kB activation was correlated with tumor invasion-related clinicopathological features such as lymphatic invasion, pathological stage, and depth of invasion (Z= 2.148, P= 0.032<0.05; t = 8.758, P= 0.033<0.05; t = 18.531, P = 0.006<0.05). NF-KB activation was significantly correlated with expression of heparanase gene (r= 0.194, P=0.046<0.05). CONCLUSION: NF-KB RelA (p65) activation was related with increased heparanase gene expression and correlated with poor clinicopathological characteristics in gastric cancers. This suggests NF-kB as a major controller of the metastatic phenotype through its reciprocal regulation of some metastasis-related genes. 展开更多
关键词 Nuclear factor kappa B Heparanase gene METASTASIS Gastric carcinoma
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Comparison of pore characteristics in the coal and shale reservoirs of Taiyuan Formation, Qinshui Basin, China 被引量:30
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作者 Yu Liu Yanming Zhu 《International Journal of Coal Science & Technology》 EI 2016年第3期330-338,共9页
Coal and shale are both unconventional gas reservoirs. Comparison of pore characteristics in shale and coal would help understand organic pore structure in shale and investigate co-exploration of shale gas and coalbed... Coal and shale are both unconventional gas reservoirs. Comparison of pore characteristics in shale and coal would help understand organic pore structure in shale and investigate co-exploration of shale gas and coalbed methane in coal bearing strata. In this study, five shale samples and three coal samples of Taiyuan Formation were collected from Qinshui Basin, China. High pressure mercury injection, scanning electronic microscopy, and fractal theory have been used to compare pore characteristics in shale and coal. The results show that pore volumes in coal are much larger than that in shale, especially pores 3-100 nm. In coal, there are many semi-closed pores in micro pores (〈10 nm) and transition pores (10-100 nm). On the contrary, micro pores and transition pores are mainly with open pores in shale. The fractal curves show that pores larger than 65 nm in coal and shale reservoir both have obvious self-similarity and the fractal dimension values in shale and coal are similar. But the fractal characteristics of pores smaller than 65 nm in shale reservoir are quite different from that in coal. 展开更多
关键词 COMPARISON COAL SHALE Organic pores FRACTAL
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Overexpression of the tissue inhibitor of metalloproteinase-3 during Xenopus embryogenesis affects head and axial tissue formation 被引量:1
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作者 Bryce PICKARD Sashko DAMJANOVSKI 《Cell Research》 SCIE CAS CSCD 2004年第5期389-399,共11页
Tissue inhibitors of metalloproteinases (TIMPs) modulate extracellular matrix remodeling during embryonic develop- ment and disease. TIMP-3 expression was examined during Xenopus laevis embryogenesis: TIMP-3 transcrip... Tissue inhibitors of metalloproteinases (TIMPs) modulate extracellular matrix remodeling during embryonic develop- ment and disease. TIMP-3 expression was examined during Xenopus laevis embryogenesis: TIMP-3 transcripts detected in the maternal pool of RNA increased at the mid-blastula transition, decreased dramatically during gastrulation and increased again during neurulation and axis elongation. Interestingly, the decrease during gastrulation was not seen in LiCl treated (dorsalized) embryos. Whole mount in situ hybridization of TIMP-3 using DIG-labeled RNA probes demonstrated that the transcripts were present in all dorsal tissues during embryogenesis, but were prominent only in head structures starting at stage 35. Overexpression of TIMP-3 through transgenesis and RNA injections led to devel- opmental abnormalities and death. Both overexpression strategies resulted in post-gastrulation perturbation including those to neural and head structures, as well as truncated axes. However, RNA injections resulted in more severe early defects such as failure of neural tube closure, and transgenesis caused truncated axes and head abnormalities. No transgenic embryo expressing TIMP-3 survived past stage 40. 展开更多
关键词 XENOPUS TIMP-3 TRANSGENESIS NEURULATION
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Scale-up of NaA zeolite membranes on α-Al_2O_3 hollow fibers by a secondary growth method with vacuum seeding 被引量:4
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作者 刘艳梅 王学瑞 +2 位作者 张玉亭 何勇 顾学红 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第7期1114-1122,共9页
NaA zeolite membranes were prepared by secondary growth method on the outer surface of α-Al2O3 hollow fiber supports. Vacuum seeding method was used for planting zeolite seeds on the support surfaces. Hydrothermal cr... NaA zeolite membranes were prepared by secondary growth method on the outer surface of α-Al2O3 hollow fiber supports. Vacuum seeding method was used for planting zeolite seeds on the support surfaces. Hydrothermal crystallization was then carried out in a synthesis solution with molar ratio of Al2O3:SiO2:Na2O:H2O = 1:2:2:120 at 100 ℃ for 4 h. Effects of seeding conditions on preparation of hollow fiber NaA zeolite membranes were extensively investigated. Moreover, hollow fiber membrane modules with packing membrane areas of ca. 0.1 and 0.2 m2 were fabricated to separate ethanol/water mixture. It is found that the thickness of seed layer is obviously affected by seed suspension concentration, coating time and vacuum degree. Close-packing seed layer is required to obtain high-quality membranes. The optimized seeding conditions (seed suspension mass concentration of 0.5%-0.7% coating time of 5 s and vacuum degree of 10 kPa) lead to dense NaA zeolite layer with a thickness of 6-8 gin. Typically, an as-synthesized hollow fiber NaA zeolite membrane exhibits good pervaporation performance with a permeation flux of 7.02 kg· m^- 2· h^- 1 and separation factor 〉 10000 for sepa- ration of 90%; (by mass) ethanol/water mixture at 75 ℃ High reproducibility has been achieved for batch-scale production of hollow fiber NaA zeolite membranes by the hydrothermal synthesis approach. 展开更多
关键词 JNaA zeolite membraneVacuum seeding methodHollow fiberPervaporation
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Effect of Mercury on the Proximate Composition of Maize (Zea mays L,)
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作者 Nathaniel Owusu Boadi Harry Okyere +2 位作者 Mercy Badu John Kenneth Mensah Idah Owusu-Appiah 《Journal of Agricultural Science and Technology(B)》 2013年第7期487-492,共6页
Plants can be exposed to mercury either by direct administration as antifungal agents, mainly to crop plants through seed treatment or foliar spray, or by accident. Mercury poisoning has become a problem of interest o... Plants can be exposed to mercury either by direct administration as antifungal agents, mainly to crop plants through seed treatment or foliar spray, or by accident. Mercury poisoning has become a problem of interest on a global scale. Natural emissions of mercury form two-thirds of the input; man-made releases form about one-third. Significant amounts of mercury may be added to agricultural land with sludge, fertilizers, lime and manures. Total mercury levels were determined in six breeds of quality protein maize using cold vapor atomic absorption spectrometry (CVAAS). The breeds analyzed were Aziga, Abeleehi, Akposoe, Golden Jubilee, Etubi and Obaatanpa. Proximate composition of these breeds was also determined. The objective of this study was therefore to assess the effect of mercury on the proximate composition of quality protein maize. The proximate composition of the maize varieties analyzed showed that the moisture content ranged from 11.57% ± 0.205% to 12.76%± 0.042%, ash from 1.11% ±0.064% to 1.58% ±0.021%, protein, 6.51%± 0.307% to 10.39% ±0.306%, fiber 1.44% ±0.071% to 1.87% ± 0.057%, fat 1.84% ± 0.078% to 2.75% ± 0.092% and carbohydrate 71.77% ± 0.035% to 76.54% ± 0.216%. The total mercury levels in the maize breeds analyzed ranged from 0.0010 + 1.17E-05μg/g to 0.0079 + 1.00E-05μg/g. The mercury levels detected were lower than the WHO limit for mercury in food of 0.5 μg/g in all the maize breeds. The low levels of mercury in the maize samples show they are safe for consumption. 展开更多
关键词 MERCURY TOXICITY MAIZE proximate composition CVAAS
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Diffusion bonding behaviour of β-γTiAl alloys containing high niobium with Ti interlayer by spark plasma sintering
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作者 Qiang GAO Lai-qi ZHANG +1 位作者 Yi QIAO Jun-pin LIN 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2022年第12期3973-3984,共12页
High niobium β-γ TiAl alloy(HNBG) was diffusion bonded using spark plasma sintering with pure Ti as interlayer. The joint microstructural evolution, growth kinetics and mechanical properties were investigated. The j... High niobium β-γ TiAl alloy(HNBG) was diffusion bonded using spark plasma sintering with pure Ti as interlayer. The joint microstructural evolution, growth kinetics and mechanical properties were investigated. The joint included three diffusion zones. The β/B2 phase formed in the Zone Ⅰ, α_(2)phase in the Zone Ⅱ, and β-Ti and α-Ti phases in the Zone Ⅲ. The thickness of β/B2 phase, the average grain size of α_(2)phase and the amount of β-Ti phase increased with the increase of bonding temperature or bonding time. The growth activation energies of β/B2 and α_(2)phases were 582 and 253 kJ/mol, respectively. The joint acquired at 1000 °C, 10 min and 10 MPa showed the maximum shear strength of 308 MPa. Fracture mainly occurred along the interfaces between Zone Ⅰ and HNBG alloy, and between Zone I and Zone Ⅱ. Fracture mechanism of the joint was characterized by brittleness rupture along the phase boundary. 展开更多
关键词 high niobiumβ-γTiAl alloy diffusion bonding spark plasma sintering INTERLAYER microstructure evolution mechanical properties
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Construction of the recombinant expression vector for CD80-IgG fusion gene and its expression in Chinese hamster ovary cells
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作者 WEI HE FANG LIU +3 位作者 LING BO LIU MIN ZHANG ZHONG BO HU PING ZOU 《Journal of Microbiology and Immunology》 2005年第4期293-300,共8页
To construct the recombinant expression functionally in Chinese hamster ovary cells in order vector for CD80-IgG fusion gene and to express it to be used as an effective method to eliminate the immune escape of leukem... To construct the recombinant expression functionally in Chinese hamster ovary cells in order vector for CD80-IgG fusion gene and to express it to be used as an effective method to eliminate the immune escape of leukemic cells, the cDNA encoding the signal and extracellular domains of murine CD80 was generated by PCR amplification from plasmid pcDNMB7 containing the full length cDNA of murine CD80 and those of murine IgG1, in which the Fc fragment was obtained through RT-PCR amplification from murine spleen cells. These two cDNAs were then cloned in tandem into eukaryotic expression vector pcDNA3.0 and the resultant recombinant plasmid pcDNA/CD80-IgG was then transfected to Chinese hamster ovary cells with liposome transfection reagent. The cell clones constitutively expressing CD80-IgG fusion protein were obtained by G418 screening. Western blotting and dot ELISA assay were used to detect the expression of the fusion protein in the supernatants of these cells. Meanwhile, the fusion protein expressed was then purified with affinity chromatography, and its biological activity was demonstrated by flow cytometry, MTr colorimetry and ELISA assay. The experimental resuits showed that these two inserts were successfully cloned into plasmid pcDNA3.0, and the highly purified fusion protein was obtained. This fusion protein was proved to be able to upregulate the density of CD80 on leukemic cells, deliberately promote the proliferative reactions of mouse allogenic lymphocytes and increase the killing activity against WEHI-3 cells from 49.7 % up to 84.6 %. In addition, this fusion protein could also enhance the IL-2 secretion from allogenic lymphocytes activated by tumorspecific antigens. It is concluded that the recombinant vector constructed can be functionally expressed in the mammalian cells, thus providing a solid foundation for the further investigation on the mechanism to eliminate the immune escape of leukemic cells in vivo. 展开更多
关键词 Gene fusion Recombinant fusion proteins Immune escape Immunotherapy
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Development Strategies of E-commerce for Small and Medium - sized Enterprises
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作者 Maarina 《International Journal of Technology Management》 2016年第9期21-22,共2页
In the economic structure of our country, small and medium-sized enterprises (SMEs) are the most important part of the backbone, with the continuous popularization of the Internet, e-commerce has gradually become a ... In the economic structure of our country, small and medium-sized enterprises (SMEs) are the most important part of the backbone, with the continuous popularization of the Internet, e-commerce has gradually become a business model that the current small and medium enterprises must face and accept, The development of enterprises is of great signifi cance. In this context, the article first introduced the importance of the development of e-commerce SMEs, and then based on the actual operation of the community put forward and analyzed the current e-commerce development under the existing problems, and finally based on the actual lack of effective Development Strategy of E - commerce for Small and Medium - sized Enterprises. 展开更多
关键词 small and medium enterprises e - commerce development strategy
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Supramolecular organizing centers(SMOCs) as signaling machines in innate immune activation 被引量:3
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作者 QIAO Qi WU Hao 《Science China(Life Sciences)》 SCIE CAS CSCD 2015年第11期1067-1072,共6页
Innate immunity offers the first line of defense against infections and other types of danger such as tumorigenesis. Its discovery provides tremendous therapeutic opportunities for numerous human diseases. Delving int... Innate immunity offers the first line of defense against infections and other types of danger such as tumorigenesis. Its discovery provides tremendous therapeutic opportunities for numerous human diseases. Delving into the structural basis of signal transduction by innate immune receptors, our lab has recently helped to establish the new paradigm in which innate immune receptors transduce ligand-binding signals through formation of higher-order assemblies containing intracellular adapters, signaling enzymes and their substrates. These large signalosome assemblies may be visible under light microscopy as punctate structures in the μm scale, connecting to the underlying molecular structures in the nm scale. They drive proximity-induced enzyme activation, and provide a mechanism for signaling amplification by nucleated polymerization. These supramolecular signaling complexes also open new questions on their cellular organization and mode of regulation, pose challenges to our methodology, and afford valuable implications in drug discovery against these medically important pathways. 展开更多
关键词 innate immunity caspase-recruitment domain pyrin domain supramolecular organizing centers FILAMENT
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Investigation of the controlled factors influencing carbon isotope composition of foxtail and common millet on the Chinese Loess Plateau 被引量:1
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作者 YANG Qing LI XiaoQiang 《Science China Earth Sciences》 SCIE EI CAS CSCD 2015年第12期2296-2308,共13页
To apply carbon isotope composition (δ13C) analyses of C4 plants to the quantitative reconstruction of paleoclimate, the functional mechanism linking plant δ13C (δ13Cp) to the environment, which is based on the... To apply carbon isotope composition (δ13C) analyses of C4 plants to the quantitative reconstruction of paleoclimate, the functional mechanism linking plant δ13C (δ13Cp) to the environment, which is based on the plants' physiological characteristics and morphological adaptability, must be thoroughly understood. Foxtail millet (Setaria italic) and common millet (Panicum miliaceum), as C4 plants, are representative crops of the rain-fed agriculture present in northern China. Fossil millets are ideal for paleoclimatic studies because of the ease of acquisition and identification to the species level. Modem seeds of foxtail and common millet collected from different habitats of the Chinese Loess Plateau, and their carbon isotope compositions, were an- alyzed and correlated with environmental factors, such as latitude, altitude, temperature, precipitation, water availability, and relative humidity. The results showed that the δ13C of foxtail millet had a significantly negative correlation with latitude (R=-0.46), which may indicate the influence of light. The effect of light on the δ13C of foxtail millet accounted for only 21% of variability, while other climatic factors did not exert significant influences. Thus, the δ13C of foxtail millet was not suitable for extracting climatic information. The δ13C of common millet was significantly and positively correlated with precipitation during the growing period (R=0.75), explaining 56% of variability. The functional mechanisms analyzed, using the plants' physiological characteristics and morphological adaptability, indicated that common millet can adapt to environmental changes because of stomatal sensitivity and some non-stomatal factors. Therefore, the 813C of common millet can record precipitation during growth and is a promising factor for paleoclimatic reconstruction. 展开更多
关键词 foxtail millet common millet stable carbon isotope water availability C4 plant physiological characteristics
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Microstructures of the interlayer in Mo/Si multilayers induced by proton irradiation
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作者 LV Peng WANG XiaoDong +4 位作者 LIU Hai ZHANG ZaiQiang GUAN JinTong CHEN Bo GUAN QingFeng 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2012年第11期2194-2198,共5页
In this work,the microstructure and optical properties of the Mo/Si multilayers mirror for the space extreme-ultraviolet solar telescope before and after 100 keV proton irradiation have been investigated.EUV/soft X-ra... In this work,the microstructure and optical properties of the Mo/Si multilayers mirror for the space extreme-ultraviolet solar telescope before and after 100 keV proton irradiation have been investigated.EUV/soft X-ray reflectometer(EXRR) results showed that,after proton irradiation,the reflectivity of the Mo/Si multilayer decreased from 12.20% to 8.34% and the center wavelength revealed red shift of 0.38 nm,as compared with those before proton irradiation.High-resolution transmission electron microscopy(HRTEM) observations revealed the presence of MoSi 2,Mo 3 Si and Mo 5 Si 3 in Mo-on-Si interlayers before irradiation.The preferred orientation such as MoSi 2 with(101) texture and Mo 5 Si 3 with(310) texture was formed in Mo-on-Si interlayers after proton irradiation,which led to the increase of thickness in the interlayers.It is suggested that the changes of microstructures in Mo/Si multilayers under proton irradiation could cause optical properties degradation. 展开更多
关键词 MULTILAYER MICROSTRUCTURE irradiation damage optical properties
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