Egg water was used to induce acrosome reaction in mature sperm of Fenneropenaeus chinensis .Transmission electron microscope and SDS PAGE were used to study the ultrastructure and protein changes of the sperm in ...Egg water was used to induce acrosome reaction in mature sperm of Fenneropenaeus chinensis .Transmission electron microscope and SDS PAGE were used to study the ultrastructure and protein changes of the sperm in F.chinensis ,which has undergone acrosome reaction.The results demonstrated that the sperms from female thelycum could be induced acrosome reaction in vitro by egg water,which was a kind of egg jelly released from oocyte into seawater during oocyte activation.More than fifty percent of sperm finished acrosome reaction during the first 30 min, in vitro .The whole event consists of the retraction of the spike and acrosome exocytosis.Spike was retracted and fused to acrosome cap followed by the release of acrosome granules.When the original egg water was diluted 5 and 10 times,The diluted egg water also have the ability to induce sperm acrosome reaction,but the acrosome reaction rate is much lower than that of the original egg water in the same time.Capillary zone electrophoresis was used to detect the biochemical components of egg water.Egg water was composed of two different components,which were also demonstrated by SDS PAGE.The molecular weights of the two kinds of egg jellies are both about 200kDa.Gelatin substrate SDS PAGE results did not show any hydrolytic enzyme activity in egg water.After acrosome reaction,many sorts of proteins in sperm are degraded.When the reacted sperms were examined with gelatin substrate SDS PAGE,there were six major peptide bands with hydrolytic enzyme activity were detected.Their molecular weights are 200kDa,130 kDa,66 kDa,53 kDa,48 kDa and 41 kDa respectively.These hydrolases cannot be detected in the sperms before acrosome reaction.The acrosome reaction of Chinese shrimp, F.chinensis is much different to that of the sperm in the shrimp, Sicyonia ingentis ,which the acrosome reaction was clearly studied.There is not filament formation during the acrosome reaction in Chinese shrimp.The time of exocytosis in the sperm of Chinese shrimp is also much longer than that of the sperm of Sicyonia ingentis .展开更多
The analytical and numerical solutions of structure and curvature of two kinds of static spherically symmetric neutron stars are calculated. The results show that Ricci tensor and curvature scalar cannot denote the cu...The analytical and numerical solutions of structure and curvature of two kinds of static spherically symmetric neutron stars are calculated. The results show that Ricci tensor and curvature scalar cannot denote the curly character of the space directly, however, to static spherically symmetric stars, these two quantities can present the relative curly degree of the space and the matter distribution to a certain extent.展开更多
We investigate neutron star properties by constructing a chiral SU(3) spontaneous breaking Lagrangian and using relativistic mean-field approximation. The results show that condensate appears at some baryon densities...We investigate neutron star properties by constructing a chiral SU(3) spontaneous breaking Lagrangian and using relativistic mean-field approximation. The results show that condensate appears at some baryon densities, and hyperons and exist in neutron star matter at high density. In this model, neutron star's maximum mass is with corresponding radius about 8 km.展开更多
The formation mechanism for the body-centered cubic structure of cluster is proposed and its total energy curve is calculated by the method of a Modified Arrangement Channel Quantum Mechanics. The energy is the funct...The formation mechanism for the body-centered cubic structure of cluster is proposed and its total energy curve is calculated by the method of a Modified Arrangement Channel Quantum Mechanics. The energy is the function of separation R between the nuclei at the center and an apex of the body-centered cubic structure. The result of the calculation shows that the curve has a minimal energy . The binding energy of with respect to was calculated to be 0.8857 a.u. This means that the cluster ofmay be formed in the body-centered cubic structure of .展开更多
The rate of the electron-capture reaction of proton, p ＋ e^- ＋ p →2 H ＋ ve, is calculated considering the temperature of solar electron in the solar center instead of that of solar ion. When the solar electron tem...The rate of the electron-capture reaction of proton, p ＋ e^- ＋ p →2 H ＋ ve, is calculated considering the temperature of solar electron in the solar center instead of that of solar ion. When the solar electron temperature is two times higher than the solar ion temperature in the solar center, the capture rate pep solar neutrino predicted by the standard solar model （SSM） is decreased to （0.16±0.01 ） SNU from （0.22±0.01 ） SNU in the chlorine solar neutrino experiment, and decreased to 2.19 SNU from 3.0 SNU in the gallium solar neutrino experiment.展开更多
文摘Egg water was used to induce acrosome reaction in mature sperm of Fenneropenaeus chinensis .Transmission electron microscope and SDS PAGE were used to study the ultrastructure and protein changes of the sperm in F.chinensis ,which has undergone acrosome reaction.The results demonstrated that the sperms from female thelycum could be induced acrosome reaction in vitro by egg water,which was a kind of egg jelly released from oocyte into seawater during oocyte activation.More than fifty percent of sperm finished acrosome reaction during the first 30 min, in vitro .The whole event consists of the retraction of the spike and acrosome exocytosis.Spike was retracted and fused to acrosome cap followed by the release of acrosome granules.When the original egg water was diluted 5 and 10 times,The diluted egg water also have the ability to induce sperm acrosome reaction,but the acrosome reaction rate is much lower than that of the original egg water in the same time.Capillary zone electrophoresis was used to detect the biochemical components of egg water.Egg water was composed of two different components,which were also demonstrated by SDS PAGE.The molecular weights of the two kinds of egg jellies are both about 200kDa.Gelatin substrate SDS PAGE results did not show any hydrolytic enzyme activity in egg water.After acrosome reaction,many sorts of proteins in sperm are degraded.When the reacted sperms were examined with gelatin substrate SDS PAGE,there were six major peptide bands with hydrolytic enzyme activity were detected.Their molecular weights are 200kDa,130 kDa,66 kDa,53 kDa,48 kDa and 41 kDa respectively.These hydrolases cannot be detected in the sperms before acrosome reaction.The acrosome reaction of Chinese shrimp, F.chinensis is much different to that of the sperm in the shrimp, Sicyonia ingentis ,which the acrosome reaction was clearly studied.There is not filament formation during the acrosome reaction in Chinese shrimp.The time of exocytosis in the sperm of Chinese shrimp is also much longer than that of the sperm of Sicyonia ingentis .
基金The project supported by National Natural Science Foundation of China under Grant No. 10275099 and the China Postdoctoral Science Foundation under Grant No. 2005037175
文摘The analytical and numerical solutions of structure and curvature of two kinds of static spherically symmetric neutron stars are calculated. The results show that Ricci tensor and curvature scalar cannot denote the curly character of the space directly, however, to static spherically symmetric stars, these two quantities can present the relative curly degree of the space and the matter distribution to a certain extent.
基金The project supported in part by the Key Research Plan of Theoretical Physics and Cross Science under Grant No.90103018National Natural Science Foundation of China under Grant No.19975062
文摘We investigate neutron star properties by constructing a chiral SU(3) spontaneous breaking Lagrangian and using relativistic mean-field approximation. The results show that condensate appears at some baryon densities, and hyperons and exist in neutron star matter at high density. In this model, neutron star's maximum mass is with corresponding radius about 8 km.
基金The project supported by National Natural Science Foundation of China(Grant No.19974027)the Foundation of Sichuan Provincial Education Committee(Grant No.01LB04)
文摘The formation mechanism for the body-centered cubic structure of cluster is proposed and its total energy curve is calculated by the method of a Modified Arrangement Channel Quantum Mechanics. The energy is the function of separation R between the nuclei at the center and an apex of the body-centered cubic structure. The result of the calculation shows that the curve has a minimal energy . The binding energy of with respect to was calculated to be 0.8857 a.u. This means that the cluster ofmay be formed in the body-centered cubic structure of .
基金Funded by the Natural Science Foundation of China (No. 10275073) and the Key Laboratory of Cosmic Ray and Astrophysics, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing.
文摘The rate of the electron-capture reaction of proton, p ＋ e^- ＋ p →2 H ＋ ve, is calculated considering the temperature of solar electron in the solar center instead of that of solar ion. When the solar electron temperature is two times higher than the solar ion temperature in the solar center, the capture rate pep solar neutrino predicted by the standard solar model （SSM） is decreased to （0.16±0.01 ） SNU from （0.22±0.01 ） SNU in the chlorine solar neutrino experiment, and decreased to 2.19 SNU from 3.0 SNU in the gallium solar neutrino experiment.
