The purpose of this study was to establish a method for determining the free concentration of ceftriaxone based on hollow fiber centrifugal ultrafiltration(HFCF-UF)technology in combination with high-performance liqui...The purpose of this study was to establish a method for determining the free concentration of ceftriaxone based on hollow fiber centrifugal ultrafiltration(HFCF-UF)technology in combination with high-performance liquid chromatography(HPLC)for free pharmacokinetic studies and the prediction of ceftriaxone concentrations in lung tissue.This method only required centrifugation for a short time,and the filtrate could be injected directly for HPLC analysis without further treatment.The specificity,linearity,precision and stability of this method were validated for quantification of free ceftriaxone.Under the optimized conditions,the absolute recoveries were more than 92.5%.The intraday and interday precision RSDs were less than 3.6%.Additionally,nonspecific adsorption(NSB)between the analyte and the ultrafiltration membrane was considered.This method was successfully applied to the analysis of the free ceftriaxone concentration in rat plasma and lung tissue.The free ceftriaxone concentration of lung tissue could be predicted by using the linear formula Cfl=Cfp(0.342 x–0.0129)(x:time).This method also provides a reliable alternative for accurate monitoring of the free ceftriaxone concentration in therapeutic drug monitoring(TDM).展开更多
文摘The purpose of this study was to establish a method for determining the free concentration of ceftriaxone based on hollow fiber centrifugal ultrafiltration(HFCF-UF)technology in combination with high-performance liquid chromatography(HPLC)for free pharmacokinetic studies and the prediction of ceftriaxone concentrations in lung tissue.This method only required centrifugation for a short time,and the filtrate could be injected directly for HPLC analysis without further treatment.The specificity,linearity,precision and stability of this method were validated for quantification of free ceftriaxone.Under the optimized conditions,the absolute recoveries were more than 92.5%.The intraday and interday precision RSDs were less than 3.6%.Additionally,nonspecific adsorption(NSB)between the analyte and the ultrafiltration membrane was considered.This method was successfully applied to the analysis of the free ceftriaxone concentration in rat plasma and lung tissue.The free ceftriaxone concentration of lung tissue could be predicted by using the linear formula Cfl=Cfp(0.342 x–0.0129)(x:time).This method also provides a reliable alternative for accurate monitoring of the free ceftriaxone concentration in therapeutic drug monitoring(TDM).
