中线癌(nuclear protein in testis carcinoma),又称NUT癌,是一种罕见的高度侵袭性恶性肿瘤,最常见于中线器官和肺。NUT癌的特征性遗传学改变是NUT癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因重排。本文将对其最常...中线癌(nuclear protein in testis carcinoma),又称NUT癌,是一种罕见的高度侵袭性恶性肿瘤,最常见于中线器官和肺。NUT癌的特征性遗传学改变是NUT癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因重排。本文将对其最常见的融合形式溴结构域蛋白4(bromodomaincontaining protein 4,BRD4)-NUTM1融合基因的致病机制以及靶向药物研发的进展进行综述。展开更多
原发性睾丸核蛋白中线癌(NUT中线癌)是伴有睾丸核蛋白(nuclear protein in testis,NUT)基因重排的一种极其罕见并具有高度侵袭性的恶性上皮细胞肿瘤,该肿瘤可发生于全身多个部位,以头颈部中线结构及纵隔多见,组织起源不明,缺乏特异性临...原发性睾丸核蛋白中线癌(NUT中线癌)是伴有睾丸核蛋白(nuclear protein in testis,NUT)基因重排的一种极其罕见并具有高度侵袭性的恶性上皮细胞肿瘤,该肿瘤可发生于全身多个部位,以头颈部中线结构及纵隔多见,组织起源不明,缺乏特异性临床表现,侵袭性强,恶性程度高,确诊时大多已属晚期,且缺乏有效治疗手段,预后极差。展开更多
伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌又称NUT癌,是一种罕见的高度侵袭性肿瘤,根据NUT中线癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因发生重排或突变定义。常规治疗方法对NUT癌效果欠佳,...伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌又称NUT癌,是一种罕见的高度侵袭性肿瘤,根据NUT中线癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因发生重排或突变定义。常规治疗方法对NUT癌效果欠佳,绝大多数患者预后差。现阶段对该基因变异的认识不足是制约NUT癌诊治取得进展的主要障碍。目前在临床实践中缺乏针对NUT癌临床特征、诊断及治疗方法的标准化指南,限制了临床医师对该疾病的充分认识。为了给临床医师提供更加明确规范的诊断参考依据,中国抗癌协会肿瘤基因诊断专业委员会中线(NUT)癌基因诊断工作组及中国抗癌协会肿瘤标志专业委员会组织了临床、病理、分子检测和生物信息分析等领域专家,综合国内外NUT癌临床应用的共识指南、重要文献及临床实践经验,共同制定了本共识,对NUT癌临床诊疗给出专家组意见,期望为临床医师提供NUT癌诊疗指导意见,降低NUT癌误诊率,提高治疗效果和预后。展开更多
伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌是一种由NUTM1(NUT midline carcinoma family member 1)基因重排定义的罕见高侵袭性肿瘤。常规治疗方法对NUT癌效果欠佳,绝大多数患者预后差。《中线(NUT)癌诊断与治疗专...伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌是一种由NUTM1(NUT midline carcinoma family member 1)基因重排定义的罕见高侵袭性肿瘤。常规治疗方法对NUT癌效果欠佳,绝大多数患者预后差。《中线(NUT)癌诊断与治疗专家共识(2023版)》于2023年10月发布,以期为临床医师提供更加明确和规范的诊治参考依据,对广大NUT癌患者有着重要意义。本文将对该共识的主要内容进行解读。展开更多
目的 探讨原发性肺睾丸核蛋白中线癌的临床特点。方法 回顾性分析1例原发性肺睾丸核蛋白中线癌患者的临床特点。以 “睾丸核蛋白”“中线癌”“肺”为检索词在万方数据库和中国期刊全文数据库进行检索,以 “nuclear protein in testis”...目的 探讨原发性肺睾丸核蛋白中线癌的临床特点。方法 回顾性分析1例原发性肺睾丸核蛋白中线癌患者的临床特点。以 “睾丸核蛋白”“中线癌”“肺”为检索词在万方数据库和中国期刊全文数据库进行检索,以 “nuclear protein in testis”“midline carcinoma”“pulmonary”为检索词在PubMed数据库进行检索,检索时间截止至2017年4月。结果 患者男,28岁,因 “双下肢乏力17d,胸痛5d”入院,影像学检查示右肺下叶背段恶性肿瘤伴区域多组淋巴结、全身多发骨转移。肺穿刺病理检查示睾丸核蛋白抗体阳性,诊断为原发性肺睾丸核蛋白中线癌,给予化疗及对症支持治疗,胸痛缓解,肿块较前缩小,目前仍在随访中。文献检索经筛选后共有24篇入选,报道31例患者,男16例,女15例,年龄5-71岁,临床表现无特异性,影像学多表现为近肺门处肿块影,多伴远处转移,脑转移罕见,中位生存期为4.1个月。结论 原发性肺睾丸核蛋白中线癌临床罕见,恶性程度高,缺乏有效治疗手段,预后差;对于年轻、无吸烟史、临床病情进展快的中央型低分化或未分化肺癌患者,应警惕该病可能。展开更多
Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptos...Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TCtreated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c.展开更多
Objective The aim of the study was to investigate the effects of Chinese herbal formula Weichang’an(WCA)on the proliferation and mitochondria-mediated apoptosis of human gastric cancer cells.Methods Cell Counting Kit...Objective The aim of the study was to investigate the effects of Chinese herbal formula Weichang’an(WCA)on the proliferation and mitochondria-mediated apoptosis of human gastric cancer cells.Methods Cell Counting Kit-8(CCK8)was used to evaluate the antiproliferative activity ofWCA on MKN45 cells;Giemsa staining was used to investigate cell colony formation;flow cytometry was used to analyze cell cycle,apoptosis rate,and caspases activation;and Hoechst staining was used to analyze the morphology of cell nuclei.The mitochondrial membrane potential was analyzed by both flow cytometric measurements and fluorescence microscopy.Western blot was used to analyze the protein levels of pro-caspase-3,Bcl-2,Bax,and Bcl-X.MKN45 cells were subcutaneously injected into the right forelimb of 16 nude mice to establish the tumor xenograft model,and a total of 12 nude mouse tumor xenograft models were successfully created.The nude mice were divided into the control group and the WCA-treated group.The two groups received normal saline and WCA treatment at 35.49 g/kg by a single daily oral gavage for 21 days.The body weight and tumor size of the nude mice were measured twice a week.The ultrastructural changes of subcutaneous tumors were observed by a transmission electron microscope.Results WCA can suppress cell proliferation and colony formation.It can also induce changes in the mitochondrial membrane potential and increase the activities of caspases-3,caspases-8,and caspases-9 in MKN45 cells.WCA induced S-phase arrest and apoptosis in MKN45 cells,and decreased the expression levels of antiapoptotic proteins Bcl-2 and Bcl-X in MKN45 cells,while increasing the expression levels of the proapoptotic proteins Bax and pro-caspase-3 compared with the control group.WCA inhibited the growth of a xenografted MKN45 tumor in nude mice,and the protein levels of Bax and pro-caspase-3 were significantly increased,while Bcl-X and Bcl-2 were reduced compared with the control group.The differences are statistically significant(p<0.05).Conclusion WCA could suppress the proliferation of gastric cancer cells via mitochondrial apoptosis.展开更多
文摘中线癌(nuclear protein in testis carcinoma),又称NUT癌,是一种罕见的高度侵袭性恶性肿瘤,最常见于中线器官和肺。NUT癌的特征性遗传学改变是NUT癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因重排。本文将对其最常见的融合形式溴结构域蛋白4(bromodomaincontaining protein 4,BRD4)-NUTM1融合基因的致病机制以及靶向药物研发的进展进行综述。
文摘原发性睾丸核蛋白中线癌(NUT中线癌)是伴有睾丸核蛋白(nuclear protein in testis,NUT)基因重排的一种极其罕见并具有高度侵袭性的恶性上皮细胞肿瘤,该肿瘤可发生于全身多个部位,以头颈部中线结构及纵隔多见,组织起源不明,缺乏特异性临床表现,侵袭性强,恶性程度高,确诊时大多已属晚期,且缺乏有效治疗手段,预后极差。
文摘伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌又称NUT癌,是一种罕见的高度侵袭性肿瘤,根据NUT中线癌家族成员1(NUT midline carcinoma family member 1,NUTM1)基因发生重排或突变定义。常规治疗方法对NUT癌效果欠佳,绝大多数患者预后差。现阶段对该基因变异的认识不足是制约NUT癌诊治取得进展的主要障碍。目前在临床实践中缺乏针对NUT癌临床特征、诊断及治疗方法的标准化指南,限制了临床医师对该疾病的充分认识。为了给临床医师提供更加明确规范的诊断参考依据,中国抗癌协会肿瘤基因诊断专业委员会中线(NUT)癌基因诊断工作组及中国抗癌协会肿瘤标志专业委员会组织了临床、病理、分子检测和生物信息分析等领域专家,综合国内外NUT癌临床应用的共识指南、重要文献及临床实践经验,共同制定了本共识,对NUT癌临床诊疗给出专家组意见,期望为临床医师提供NUT癌诊疗指导意见,降低NUT癌误诊率,提高治疗效果和预后。
文摘伴睾丸核蛋白(nuclear protein in testis,NUT)基因重排的中线癌是一种由NUTM1(NUT midline carcinoma family member 1)基因重排定义的罕见高侵袭性肿瘤。常规治疗方法对NUT癌效果欠佳,绝大多数患者预后差。《中线(NUT)癌诊断与治疗专家共识(2023版)》于2023年10月发布,以期为临床医师提供更加明确和规范的诊治参考依据,对广大NUT癌患者有着重要意义。本文将对该共识的主要内容进行解读。
基金supported by Grant 39825115 of the National Outstanding Young Scientists,a special grant of major state basic research program of China(No.G1999053901)a grant from the Chinese Academy of Sciences KSCX2-SW-203 to JR Wu.
文摘Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TCtreated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c.
基金funded by the Three-Year Plan of Action for the Development of Traditional Chinese Medicine in Shanghai(ZY3-CCCX-3-2003)State Administration of Traditional Chinese Medicine(TCM)Clinical Research Base of China(JDZX2015068)+1 种基金the Key and Promotion Special Topics of Henan(202102310164)Henan Province Traditional Chinese Medicine Scientific Research Special Project(2022ZY2031).
文摘Objective The aim of the study was to investigate the effects of Chinese herbal formula Weichang’an(WCA)on the proliferation and mitochondria-mediated apoptosis of human gastric cancer cells.Methods Cell Counting Kit-8(CCK8)was used to evaluate the antiproliferative activity ofWCA on MKN45 cells;Giemsa staining was used to investigate cell colony formation;flow cytometry was used to analyze cell cycle,apoptosis rate,and caspases activation;and Hoechst staining was used to analyze the morphology of cell nuclei.The mitochondrial membrane potential was analyzed by both flow cytometric measurements and fluorescence microscopy.Western blot was used to analyze the protein levels of pro-caspase-3,Bcl-2,Bax,and Bcl-X.MKN45 cells were subcutaneously injected into the right forelimb of 16 nude mice to establish the tumor xenograft model,and a total of 12 nude mouse tumor xenograft models were successfully created.The nude mice were divided into the control group and the WCA-treated group.The two groups received normal saline and WCA treatment at 35.49 g/kg by a single daily oral gavage for 21 days.The body weight and tumor size of the nude mice were measured twice a week.The ultrastructural changes of subcutaneous tumors were observed by a transmission electron microscope.Results WCA can suppress cell proliferation and colony formation.It can also induce changes in the mitochondrial membrane potential and increase the activities of caspases-3,caspases-8,and caspases-9 in MKN45 cells.WCA induced S-phase arrest and apoptosis in MKN45 cells,and decreased the expression levels of antiapoptotic proteins Bcl-2 and Bcl-X in MKN45 cells,while increasing the expression levels of the proapoptotic proteins Bax and pro-caspase-3 compared with the control group.WCA inhibited the growth of a xenografted MKN45 tumor in nude mice,and the protein levels of Bax and pro-caspase-3 were significantly increased,while Bcl-X and Bcl-2 were reduced compared with the control group.The differences are statistically significant(p<0.05).Conclusion WCA could suppress the proliferation of gastric cancer cells via mitochondrial apoptosis.
