To clone the DL-6 gene, positive and negative cross combinations were developed between dl-6 and 9311; based on the genetic analysis, it was found that drooping leaf was controlled by one recessive nuclear gene DL-6, ...To clone the DL-6 gene, positive and negative cross combinations were developed between dl-6 and 9311; based on the genetic analysis, it was found that drooping leaf was controlled by one recessive nuclear gene DL-6, DL-6 was pri- marily mapped on the short arm of chromosome 3 with SSR markers, finally the DL-6 gene was fine mapped in the 85 kb section between markers 13-5 and 13-8 using newly developed InDel marker; the open reading frames （ORFs） in the sec- tion were analyzed and found that YABBY gene coded by ORF9 might be a droop- ing leaf-related gene. YABBY genes in mutant dl-6 and in wild type were se- quenced, and the sequencing results were compared with Nipponbare sequence, and showed that 1 bp mutation was found in first exon of YABBY gene in the mu- tant dl-6, which caused the coded cysteine of the wild type become the arginine of the mutant; at the same time, 8 bp deletion was also found at 3＇ end of ORF9 gene. These two mutations which one was the functional mutation of dl-6 has been still uncertain and needed further research.展开更多
基金Supported by the Key Project of National Science and Technology Support Plan(2011BAD16B03)Postgraduate Innovation Project of Jiangsu Province(CXZZ12_0906)+2 种基金the Project of Agricultural Independent Innovation Fund in Jiangsu Province[CX(12)1003]Natural Science Fund in Jiangsu Province(BK20130725)the Project of Science and Technology Support Program in Jiangsu Province(BE2012309)~~
文摘To clone the DL-6 gene, positive and negative cross combinations were developed between dl-6 and 9311; based on the genetic analysis, it was found that drooping leaf was controlled by one recessive nuclear gene DL-6, DL-6 was pri- marily mapped on the short arm of chromosome 3 with SSR markers, finally the DL-6 gene was fine mapped in the 85 kb section between markers 13-5 and 13-8 using newly developed InDel marker; the open reading frames （ORFs） in the sec- tion were analyzed and found that YABBY gene coded by ORF9 might be a droop- ing leaf-related gene. YABBY genes in mutant dl-6 and in wild type were se- quenced, and the sequencing results were compared with Nipponbare sequence, and showed that 1 bp mutation was found in first exon of YABBY gene in the mu- tant dl-6, which caused the coded cysteine of the wild type become the arginine of the mutant; at the same time, 8 bp deletion was also found at 3＇ end of ORF9 gene. These two mutations which one was the functional mutation of dl-6 has been still uncertain and needed further research.
