试论中药材规范化种植与中药材指纹图谱

探讨了中药材规范化种植与中药材指纹图谱的关系 ,认为中药材规范化种植是制定中药材指纹图谱的前提条件 ,同时中药材指纹图谱拉动中药材规范化种植的实施 ,也是药材生产管理规范

2种颜色拳参饮片HPLC-MS指纹图谱比较研究

目的:商品拳参饮片有紫红色和棕红色2种颜色,建立拳参饮片HPLC-MS指纹图谱,以了解2种不同颜色的拳参饮片中除绿原酸及没食子酸外,所含其他成分是否异同。方法:采用Inertsil ODS-SP C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相为1%甲酸水溶液(A)^(-1)%甲酸乙腈(B),梯度洗脱,流速0.8 m L·min^(-1),检测波长290 nm,柱温40℃;利用《中药色谱指纹图谱相似度评价系统A版》软件处理分析及多元统计等方法分析,采用高分辨质谱(离子源电离模式:ESI±;加热块温度:200℃;雾化器流量:N2,1.5 L·min^(-1))对部分共有峰进行鉴定。结果:对2种颜色拳参饮片的指纹图谱进行评价,确定了15个共有峰,指认出其中5个峰分别对应为山柰酚、24(E)-亚乙基环木菠萝烷酮-3α-醇、原儿茶酸、绿原酸及没食子酸;比较山柰酚、24(E)-亚乙基环木菠萝烷酮-3α-醇、原儿茶酸、绿原酸4个化学成分的色谱峰面积,紫红色拳参饮片中4个成分色谱峰面积均大于棕红色饮片。结论:本实验建立拳参饮片的HPLC-MS指纹图谱,结果稳定可靠。紫红色与棕红色拳参饮片所含化学成分相似,但主要成分含量存在明显差异。

A Molecular Approach to Identification of the Chinese Drug“pu Gong Ying”(Herba Taraxaci)and Six Adulterants byDNA Fingerprinting Using Random Primed PolymeraseChain Resaction(PCR)

DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.