A Molecular Approach to Identification of the Chinese Drug“pu Gong Ying”(Herba Taraxaci)and Six Adulterants byDNA Fingerprinting Using Random Primed PolymeraseChain Resaction(PCR)

DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.

超临界CO_2萃取与石油醚提取蒲公英的比较分析

目的:比较2种不同方法提取中药材蒲公英的挥发油化学成分。方法:采用超临界CO_2萃取法和石油醚提取法提取蒲公英,并用气相色谱-质谱联用技术对其化学成分进行了分析。结果:从超临界CO_2萃取出的蒲公英样品中分离鉴定出26种化学成分,占总质量的77.66%;从石油醚提取的蒲公英样品中共鉴定出27种化合物,占总质量的87.23%;超临界CO_2萃取法提取的蒲公英样品中酸类物质最多,占总质量的36.4%;而石油醚提取的样品中,除去含量最多的9,12,15-十八碳三烯醛外,则是以酯类物质最多,占总质量的27.68%。结论:2种方法提取的蒲公英样品化学成分各有侧重,与石油醚提取相比,超临界CO_2萃取具有提取温度低、溶剂残留少等优点,并可用于提取蒲公英中挥发性和半挥发性酸类成分。