To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water co...To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.展开更多
Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), II...Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), III(3), 3-O-[b-D-glucopyranosyl(12)-b-D-glucopyranosyl]-16a-ethoxy-olean-12-ene-28-oic acid-28-O-b-D-glu-copyranoside(4) and 3-O-[(2-O-sulfuryl-b-D-glucopyranosyl)(12)-b-D-glucopyranosyl]-echinocystic acid-28-O-b-D-glucopyranoside(5). Compounds 4 and 5 are new compounds and named eclalbasaponins XI and XII, respectively. Compounds 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.展开更多
To study the chemical constituents of Hedysarum gmelinii. Methods Theconstituents were separated and purified by different methods of chromatography, and theirstructures were elucidated by DR, MS and NMR. Results Eigh...To study the chemical constituents of Hedysarum gmelinii. Methods Theconstituents were separated and purified by different methods of chromatography, and theirstructures were elucidated by DR, MS and NMR. Results Eight compounds were isolated from Hedysarumgmelinii, including three triteipenoids, two flavonoids and two other compounds. Their structureswere identified as squasapogenol (1), soyasapogenol (2), lupeol (3), 3, 9-dihydroxy coumestan (4),3-hydroxy-9-me-thoxy pterocarpan (5), β-sitosterol (6), palmatic acid (7), and hexadecanoic acid 2,3-dihydroxypropyl ester (8). Conclusion All the compounds have been isolated from this plant forthe first time. Compounds 1 — 4 and 8 were obtained from this genus for the first time. The NMRdata of 1 are reported for the first time.展开更多
基金NationalBasicResearchProgramofChina (No .G19990 5 44 0 6)NationalNaturalScienceFoundationofChina(No .3 9970 898)
文摘To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.
文摘Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), III(3), 3-O-[b-D-glucopyranosyl(12)-b-D-glucopyranosyl]-16a-ethoxy-olean-12-ene-28-oic acid-28-O-b-D-glu-copyranoside(4) and 3-O-[(2-O-sulfuryl-b-D-glucopyranosyl)(12)-b-D-glucopyranosyl]-echinocystic acid-28-O-b-D-glucopyranoside(5). Compounds 4 and 5 are new compounds and named eclalbasaponins XI and XII, respectively. Compounds 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.
基金National Natural Science Foundation of China (20432030)
文摘To study the chemical constituents of Hedysarum gmelinii. Methods Theconstituents were separated and purified by different methods of chromatography, and theirstructures were elucidated by DR, MS and NMR. Results Eight compounds were isolated from Hedysarumgmelinii, including three triteipenoids, two flavonoids and two other compounds. Their structureswere identified as squasapogenol (1), soyasapogenol (2), lupeol (3), 3, 9-dihydroxy coumestan (4),3-hydroxy-9-me-thoxy pterocarpan (5), β-sitosterol (6), palmatic acid (7), and hexadecanoic acid 2,3-dihydroxypropyl ester (8). Conclusion All the compounds have been isolated from this plant forthe first time. Compounds 1 — 4 and 8 were obtained from this genus for the first time. The NMRdata of 1 are reported for the first time.
