中药解毒通络法对急性心肌梗死患者C反应蛋白的干预

目的:了解中药口服液对C反应蛋白(CRP)的影响。方法:60例急性心肌梗死患者随机分为治疗组32例和对照组28例。治疗组在常规西医治疗的基础上加用中药口服液;对照组常规西医治疗。治疗前后分别测定CRP、血脂(总胆固醇TC、甘油三脂TG、高密度脂蛋白胆固醇HDL-C、低密度脂蛋白胆固醇LDL-C、肝功、肾功。结果:1个月后治疗组与对照组CRP分别降低了33.5%和6.3%,TC分别降低了20.5%和3.1%,LDL-C分别降低了23.7%和1.8%,HDL-C分别升高了3.8%和0.9%。结论:中药口服药与常规西医治疗具有协同作用。

浅谈D2C130-A型中药煎煮机与直火加热法制备中药汤剂的对比研究

目的:比较D2C130-A型中药煎煮机与直火加热法制备中药汤剂的质量。方法:以固体含量的微生物限度为指标,比较两种方法制备中药汤剂的质量。结果:D2C130-A型中药煎煮机制备中药汤剂的总固体含量是直火加热法制备中药汤剂的2倍,保存时间较直火加热法制备的中药汤剂长。结论:D2C130-A型中药煎煮机制备中药汤剂质量优于直火加热法,适宜临床应用。

健脾祛痰化瘀方对氧化型低密度脂蛋白诱导血管细胞信号分子钙离子和蛋白激酶C表达的影响

为了探讨健脾祛痰化瘀方在抗氧化型低密度脂蛋白致动脉粥样硬化中对信号转导分子钙离子和蛋白激酶C的影响 ,分别以健脾祛痰化瘀方—沥水调脂胶囊含药血清 (浓度分别为 5 %、10 %和 2 0 % )、氧化型低密度脂蛋白 (10 0mg L)处理人脐静脉内皮细胞和人脐动脉平滑肌细胞 ,以流式细胞仪检测两种细胞胞质游离钙离子浓度 ,以液体闪烁仪检测平滑肌细胞胞膜蛋白激酶C活性。结果发现 ,沥水调脂胶囊含药血清对氧化型低密度脂蛋白引起的内皮细胞、平滑肌细胞胞质游离钙离子浓度升高及平滑肌细胞胞膜蛋白激酶C活性升高有明显的抑制作用 ,其中 2 0 %含药血清作用最为明显。结果提示 ,健脾祛痰化瘀方可能通过调节钙离子、蛋白激酶C两种信号传递分子的变化起抗氧化作用 ,进而抑制内皮细胞凋亡及平滑肌细胞增殖 ,达到阻止动脉粥样硬化发生的作用。

Evaluation of antileishmanial,antibacterial and brine shrimp cytotoxic potential of crude methanolic extract of Herb Ocimum basilicum(Lamiacea)

OBJECTIVE:To collect and screen for ethnopharmacological properties(antileishmanial,antibacterial and brine lethality assays) of medicinal plant Ocimum basilicum from Peshawar region(34.008 latitude and 71.57 altitudes).METHODS:In the present study a general antileishmanial activity against Leishmania tropica strain was carried out.The antibacterial potential of the plant was performed against 06 gram positive and 06 gram negative bacteria.Brine shrimp cytotoxicity assay at different concentrations were investigated.RESULTS:The anti-promastigotes profile of the plant showed good antileishmanial activity exhibited LC_(50) value 21.67 μg/mL.The result for gram positive antibacterial activity revealed that the O.basilicum leaves extract possesses significant inhibitory activity at highest two concentrations ranging from20.66 ± 0.31 to 31.86 ± 0.80 for Clostridium perfringens type C and Bacillus subtitilis,respectively,as compared to the gentamycin(27.36 ± 0.55 and21.80 ± 0.72,respectively).For gram negative bacteria good activity was observed.A highest zone of inhibition was recorded for Pseudomonas aeroginosa(28.83 ± 0.28) atthe highest concentration(10 mg/mL).The LC_(50) value obtained for brine shrimp lethality assay was 91.56 μg/mL.CONCLUSION:The herb basil possesses effective cidal activities which make this plant a good candidate for the isolation of antiprotozoal and antibacterial compounds which may lead to the development of novel drug.