A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the du...A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the dual-wavelength colorimetric method, and genotype of Waxygenes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L （intron 5-exon 7 and intron 8–9） andWx-S （exon 9–intron 10） revealed several polymorphisms （S0, S？15, LC, LF, LY）. Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S？15 allele had waxy endosperm starch with the amylose content range 0–2.58% and 64 accessions with the S0 allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%. Five genotypes including So15/LF （45% of the accessions）, S0/LF （25%）, S0/LY （12%）, S0/LC （11%）, and S？15/LY （7%） were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.展开更多
基金supported by the National Natural Science Foundation of China(31271791)Research Project supported by Shanxi Scholarship Council of China(2016-066)+4 种基金the Subject Construction Project of Anhui Academy of Agricultural Sciences(16A0102)China Agriculture Research System(CARS-06-13.5-A16)the Key Research and Development Program(General Project)(Agriculture)of Shanxi Province of China(201603D221003-5)the Special Program of Crop Germplasm Resources Protection and Utilization of Ministry of Agriculture of the People's Republic of China(2017NWB036-21)the Subplatform Project of National Proso Millet Germplasm Resources of China(NICGR2017-027)
文摘A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the dual-wavelength colorimetric method, and genotype of Waxygenes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L （intron 5-exon 7 and intron 8–9） andWx-S （exon 9–intron 10） revealed several polymorphisms （S0, S？15, LC, LF, LY）. Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S？15 allele had waxy endosperm starch with the amylose content range 0–2.58% and 64 accessions with the S0 allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%. Five genotypes including So15/LF （45% of the accessions）, S0/LF （25%）, S0/LY （12%）, S0/LC （11%）, and S？15/LY （7%） were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.
