Bat coronavirus(CoV)RaTG13 shares the highest genome sequence identity with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)among all known coronaviruses,and also uses human angiotensin converting enzyme 2(...Bat coronavirus(CoV)RaTG13 shares the highest genome sequence identity with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)among all known coronaviruses,and also uses human angiotensin converting enzyme 2(hACE2)for virus entry.Thus,SARS-CoV-2 is thought to have originated from bat.However,whether SARS-CoV-2 emerged from bats directly or through an intermediate host remains elusive.Here,we found that Rhinolophus affinis bat ACE2(Ra ACE2)is an entry receptor for both SARSCoV-2 and Ra TG13,although the binding of Ra ACE2 to the receptor-binding domain(RBD)of SARSCoV-2 is markedly weaker than that of h ACE2.We further evaluated the receptor activities of ACE2 s from additional 16 diverse animal species for Ra TG13,SARS-CoV,and SARS-CoV-2 in terms of S protein binding,membrane fusion,and pseudovirus entry.We found that the Ra TG13 spike(S)protein is significantly less fusogenic than SARS-CoV and SARS-CoV-2,and seven out of sixteen different ACE2 s function as entry receptors for all three viruses,indicating that all three viruses might have broad host rages.Of note,Ra TG13 S pseudovirions can use mouse,but not pangolin ACE2,for virus entry,whereas SARS-CoV-2 S pseudovirions can use pangolin,but not mouse,ACE2 enter cells efficiently.Mutagenesis analysis revealed that residues 484 and 498 in Ra TG13 and SARS-CoV-2 S proteins play critical roles in recognition of mouse and human ACE2 s.Finally,two polymorphous Rhinolophous sinicus bat ACE2 s showed different susceptibilities to virus entry by Ra TG13 and SARS-CoV-2 S pseudovirions,suggesting possible coevolution.Our results offer better understanding of the mechanism of coronavirus entry,host range,and virushost coevolution.展开更多
Extreme rainstorm and the subsequent flood increasingly threaten the security of human society and ecological environment with aggravation of global climate change and anthropogenic activity in recent years. Therefore...Extreme rainstorm and the subsequent flood increasingly threaten the security of human society and ecological environment with aggravation of global climate change and anthropogenic activity in recent years. Therefore, the research on flood mitigation service(FMS) of ecosystem should be paid more attention to mitigate the risk. In this paper, we assessed FMS in the Upper Reaches of Hanjiang River(URHR), China from 2000 to 2014 using the Soil Conservation Service Curve Number(SCS-CN) model, and further simulated the future FMS under two climate scenarios(in 2020 and 2030). The results reveal that the FMS presented a fluctuating rising trend in the URHR from 2000 to 2014. The FMS in southern URHR was higher than that of northern URHR, and the change rate of FMS in the upstream of URHR(western URHR) was higher than the downstream of URHR(eastern URHR). The future FMS under scenarios of Medium-High Emissions(A2) and Medium-Low Emissions(B2) will decrease consistently. As land use/land cover changes in the URHR are negligible, we concluded that the change in FMS was mainly driven by climate change, such as storm and runoff. Our study highlights that climate scenarios analysis should be incorporated into the assessment of hydrologic-related services to facilitate regional water resources management.展开更多
The bilayer compounds[n-CnH2n+1N(CH3)3]2Zn Cl4(n=16,18)experience solid-solid phase transition within the temperature range of 310 to 340 K.The low-temperature crystal structures of the pure compounds are characterist...The bilayer compounds[n-CnH2n+1N(CH3)3]2Zn Cl4(n=16,18)experience solid-solid phase transition within the temperature range of 310 to 340 K.The low-temperature crystal structures of the pure compounds are characteristic of the piling effect in which a 2D macro-anion Zn Cl42- is sandwiched between two alkylammonium layers.These layers become conformationally disordered in the high-temperature phases.The structures can alternatively be viewed as a double layer of alkylammonium ions between Zn Cl42- sheets and can be considered as crystalline models of lipid bilayers.The experimental subsolidus binary phase diagram of[n-C16H33N(CH3)3]2Zn Cl4-[n-C18H37N(CH3)3]2Zn Cl4 has also been established over the whole composition range by differential scanning calorimetry(DSC)and X-ray diffraction.In the phase diagram,one intermediate compound[n-C16H33N(CH3)3][n-C18H37N(CH3)3]Zn Cl4 at WC16C3Zn%47.50 and two eutectoid invariants points at WC16C3Zn%35.10 and75.70 were observed;the respective temperatures of the two eutectoids are 320±1 and 315±1 K.In addition,there are three noticeable solid solution ranges in the phase diagram:α-phase at the left,β-phase at the right,andγ-phase in the middle.展开更多
Since the last decade the central government endeavors to promote large scale of urbanization in China.Lots of new urban towns and districts are taking shape. Outline of the 12th Five-year Plan released by the central...Since the last decade the central government endeavors to promote large scale of urbanization in China.Lots of new urban towns and districts are taking shape. Outline of the 12th Five-year Plan released by the central government points out that the new towns and districts are to be regulated. But few researches are considering the new towns and districts especially in the spatial distributive pattern and status in China. Given this situation this paper, after sorting out the world-wide research and development of new urban towns and districts, gives definitions of broad and narrow meanings. Based on the broad meaning definition, the database of new towns and districts in China is established. This paper then conducts a preliminary analysis of the new towns and districts.Then the two-step work plan and the advices are also proposed to further improve the research and practice of its kind in China.展开更多
基金supported by the National Key R&D Program of China(2020YFA0707600 and 2020YFC0841000)the National Natural Science Foundation of China(31970171 and 31670164)the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2016-12M-1-014 and 2020-12M-Co V19-010)。
文摘Bat coronavirus(CoV)RaTG13 shares the highest genome sequence identity with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)among all known coronaviruses,and also uses human angiotensin converting enzyme 2(hACE2)for virus entry.Thus,SARS-CoV-2 is thought to have originated from bat.However,whether SARS-CoV-2 emerged from bats directly or through an intermediate host remains elusive.Here,we found that Rhinolophus affinis bat ACE2(Ra ACE2)is an entry receptor for both SARSCoV-2 and Ra TG13,although the binding of Ra ACE2 to the receptor-binding domain(RBD)of SARSCoV-2 is markedly weaker than that of h ACE2.We further evaluated the receptor activities of ACE2 s from additional 16 diverse animal species for Ra TG13,SARS-CoV,and SARS-CoV-2 in terms of S protein binding,membrane fusion,and pseudovirus entry.We found that the Ra TG13 spike(S)protein is significantly less fusogenic than SARS-CoV and SARS-CoV-2,and seven out of sixteen different ACE2 s function as entry receptors for all three viruses,indicating that all three viruses might have broad host rages.Of note,Ra TG13 S pseudovirions can use mouse,but not pangolin ACE2,for virus entry,whereas SARS-CoV-2 S pseudovirions can use pangolin,but not mouse,ACE2 enter cells efficiently.Mutagenesis analysis revealed that residues 484 and 498 in Ra TG13 and SARS-CoV-2 S proteins play critical roles in recognition of mouse and human ACE2 s.Finally,two polymorphous Rhinolophous sinicus bat ACE2 s showed different susceptibilities to virus entry by Ra TG13 and SARS-CoV-2 S pseudovirions,suggesting possible coevolution.Our results offer better understanding of the mechanism of coronavirus entry,host range,and virushost coevolution.
基金Natural Science Basic Research Plan in Shaanxi Province of China,No.2017JQ4009National Natural Science Foundation of China,No.41601182,No.41471097+4 种基金National Social Science Foundation of China,No.14AZD094Key Project of Chinese Ministry of Education,No.15JJD790022The National Key Research and Development Plan of China,No.2016YFC0501601The Science and Technology Service Network Initiative Project of Chinese Academy of Sciences,No.KFJ-STS-ZDTP-036Fundamental Research Funds for the Central University,No.GK201703053
文摘Extreme rainstorm and the subsequent flood increasingly threaten the security of human society and ecological environment with aggravation of global climate change and anthropogenic activity in recent years. Therefore, the research on flood mitigation service(FMS) of ecosystem should be paid more attention to mitigate the risk. In this paper, we assessed FMS in the Upper Reaches of Hanjiang River(URHR), China from 2000 to 2014 using the Soil Conservation Service Curve Number(SCS-CN) model, and further simulated the future FMS under two climate scenarios(in 2020 and 2030). The results reveal that the FMS presented a fluctuating rising trend in the URHR from 2000 to 2014. The FMS in southern URHR was higher than that of northern URHR, and the change rate of FMS in the upstream of URHR(western URHR) was higher than the downstream of URHR(eastern URHR). The future FMS under scenarios of Medium-High Emissions(A2) and Medium-Low Emissions(B2) will decrease consistently. As land use/land cover changes in the URHR are negligible, we concluded that the change in FMS was mainly driven by climate change, such as storm and runoff. Our study highlights that climate scenarios analysis should be incorporated into the assessment of hydrologic-related services to facilitate regional water resources management.
基金financially supported by the National Natural Science Foundation of China(21473048,21246006)the Natural Science Foundation of Hebei Province(B2012205034)the Science Foundation of Hebei Normal University(L2011K04,L2013B07)
文摘The bilayer compounds[n-CnH2n+1N(CH3)3]2Zn Cl4(n=16,18)experience solid-solid phase transition within the temperature range of 310 to 340 K.The low-temperature crystal structures of the pure compounds are characteristic of the piling effect in which a 2D macro-anion Zn Cl42- is sandwiched between two alkylammonium layers.These layers become conformationally disordered in the high-temperature phases.The structures can alternatively be viewed as a double layer of alkylammonium ions between Zn Cl42- sheets and can be considered as crystalline models of lipid bilayers.The experimental subsolidus binary phase diagram of[n-C16H33N(CH3)3]2Zn Cl4-[n-C18H37N(CH3)3]2Zn Cl4 has also been established over the whole composition range by differential scanning calorimetry(DSC)and X-ray diffraction.In the phase diagram,one intermediate compound[n-C16H33N(CH3)3][n-C18H37N(CH3)3]Zn Cl4 at WC16C3Zn%47.50 and two eutectoid invariants points at WC16C3Zn%35.10 and75.70 were observed;the respective temperatures of the two eutectoids are 320±1 and 315±1 K.In addition,there are three noticeable solid solution ranges in the phase diagram:α-phase at the left,β-phase at the right,andγ-phase in the middle.
文摘Since the last decade the central government endeavors to promote large scale of urbanization in China.Lots of new urban towns and districts are taking shape. Outline of the 12th Five-year Plan released by the central government points out that the new towns and districts are to be regulated. But few researches are considering the new towns and districts especially in the spatial distributive pattern and status in China. Given this situation this paper, after sorting out the world-wide research and development of new urban towns and districts, gives definitions of broad and narrow meanings. Based on the broad meaning definition, the database of new towns and districts in China is established. This paper then conducts a preliminary analysis of the new towns and districts.Then the two-step work plan and the advices are also proposed to further improve the research and practice of its kind in China.
