In order to monitor malt quality in the malting industry, despite yearly variations in the barley quality, 394 barley samples were analysed using conventional (moisture, protein and B-glucan content) and mid-infrare...In order to monitor malt quality in the malting industry, despite yearly variations in the barley quality, 394 barley samples were analysed using conventional (moisture, protein and B-glucan content) and mid-infrared Fourier transform spectroscopy FT-IR. The experimental dataset included barley from three harvest years, two barley species, 77 barley varieties, and two-row and six-row barley, from 16 cultivation sites. For each sample, the malt quality indices were also assessed according to European Brewing Convention (EBC) standards. Principal component analysis (PCA) was carried out on mean-centred, normalized and derivative spectra using 200/cm width spectral bands. The most informative spectral bands were observed in the 800-1,000/cm and 1,000-1,200/cm ranges. PCA revealed that barley harvested in 2010 and in 2011 had bands that were very close together, while 2009 harvest clearly displayed a difference in its quality. PCA made it possible to distinguish two species and confirmed that two-row winter barley quality was closer to two-row spring barley quality than to six-row winter barley. Results indicate that mid-infrared spectrometry (MIR) could be a very useful and rapid analytical tool to assess barley qualitative quality.展开更多
We studied the depth distribution of periphyton,growing on inundated dead trees in Kenyir Lake,Malaysia in June 1995.The algal floral composition and structure manifested changes down the depth gradient in terms of sp...We studied the depth distribution of periphyton,growing on inundated dead trees in Kenyir Lake,Malaysia in June 1995.The algal floral composition and structure manifested changes down the depth gradient in terms of species richness,abundance,diversity and cell density.In regression analysis,all these algal attributes were negatively correlated with the depth gradients at P<0.05.In terms of species richness,the bacillariophytes showed dominance over the cyanophytes and chlorophytes;whereas with respect to standing crop,the cyanophytes showed dominance over the bacillariophytes and chlorophytes.The chlorophyll a was higher at the mid and bottom-depths than the surface-depth in both the downstream and upstream sites,which showed that vertical productivity or biomass accumulation was greater in low light irradiance.The product-moment correlation analysis showed that conductivity,turbidity,dissolved oxygen,reactive phosphate and ammonium-nitrogen were highly correlated with the algal assemblage data.However,photosynthetic active radiation(PAR) showed poor correlation with the community data.These observations have cast some light on the autoecological characteristics,habitat preferences and environmental responses of tropical periphytic communities.展开更多
Flow cytometric determinations of the abundance distribution picophytoplankton (i.e. Prochlorococcus spp., orange fluorescence and community structure of Svnechococcus spp. and picoeukaryotes) were used for samples ...Flow cytometric determinations of the abundance distribution picophytoplankton (i.e. Prochlorococcus spp., orange fluorescence and community structure of Svnechococcus spp. and picoeukaryotes) were used for samples taken from the Philippine Sea in the western tropical Pacific Ocean from September to October of 2004. A fluorescence probe was employed to detect Chlorophyll a (Chl a). Abundances of Prochlorococcus spp., orange fluorescence Synechococcus spp. and picoeukaryotes ranged from 0.1 to 58×10^3 cells ml^-1, 0.38 to 17×10^2 cells ml^-1 and 0.42 to 26×10^2 cells ml^-1, respectively. Synechococcus spp. and picoeukaryotes co-occurred in relatively shallow water with the maximum abundance observed at 50 to 70 m depth, while Prochlorococcus spp. only occurred in the 70 to 200 m layer. Prochlorococcus spp. was the dominant picophytoplankton population in terms of abundance and biomass. The cell size and carbon biomass content were estimated for the three picophytoplankton groups. In addition, among the three groups of picophytoplankton, the relative contribution of red fluorescence to the total red fluorescence varied with depth. The fluorescence and light scatter properties of individual cells indicated that in the upper 100 m layer, picoeukaryotes were a major contributor to total red fluorescence, while at the depth below 100 m, Prochlorococcus spp. and Synechococcus spp. made an important contribution to the total red fluorescence.展开更多
Virioplankton dynamics at seven sample sites(from two freshwater lakes) with different trophic states were monitored over the period of a year.Water was sampled monthly from August 2004 to July 2005,and the abundances...Virioplankton dynamics at seven sample sites(from two freshwater lakes) with different trophic states were monitored over the period of a year.Water was sampled monthly from August 2004 to July 2005,and the abundances of viruses and bacteria were measured using direct epifluorescence microscopy counting.Results indicated that both natural and anthropogenic factors could influence the distribution of virioplankton.Temporally,viral abundance was significantly correlated to bacterial density and water temperature.Spatially,viral abundance was significantly correlated to trophic state.This in turn indicated that viral abundance was directly dependant on host abundance in eutrophic lakes,while trophic state and temperature could drive the distribution of virioplankton.The virus-bacteria ratio was significantly lower in less productive water-bodies.The result implied that the control of virioplankton on their hosts may change according to the host density.展开更多
文摘In order to monitor malt quality in the malting industry, despite yearly variations in the barley quality, 394 barley samples were analysed using conventional (moisture, protein and B-glucan content) and mid-infrared Fourier transform spectroscopy FT-IR. The experimental dataset included barley from three harvest years, two barley species, 77 barley varieties, and two-row and six-row barley, from 16 cultivation sites. For each sample, the malt quality indices were also assessed according to European Brewing Convention (EBC) standards. Principal component analysis (PCA) was carried out on mean-centred, normalized and derivative spectra using 200/cm width spectral bands. The most informative spectral bands were observed in the 800-1,000/cm and 1,000-1,200/cm ranges. PCA revealed that barley harvested in 2010 and in 2011 had bands that were very close together, while 2009 harvest clearly displayed a difference in its quality. PCA made it possible to distinguish two species and confirmed that two-row winter barley quality was closer to two-row spring barley quality than to six-row winter barley. Results indicate that mid-infrared spectrometry (MIR) could be a very useful and rapid analytical tool to assess barley qualitative quality.
基金Supported by the Goverment of Malaysia,Intensified Research in Priority Areas(IRPA Project)(No.50258-J3)
文摘We studied the depth distribution of periphyton,growing on inundated dead trees in Kenyir Lake,Malaysia in June 1995.The algal floral composition and structure manifested changes down the depth gradient in terms of species richness,abundance,diversity and cell density.In regression analysis,all these algal attributes were negatively correlated with the depth gradients at P<0.05.In terms of species richness,the bacillariophytes showed dominance over the cyanophytes and chlorophytes;whereas with respect to standing crop,the cyanophytes showed dominance over the bacillariophytes and chlorophytes.The chlorophyll a was higher at the mid and bottom-depths than the surface-depth in both the downstream and upstream sites,which showed that vertical productivity or biomass accumulation was greater in low light irradiance.The product-moment correlation analysis showed that conductivity,turbidity,dissolved oxygen,reactive phosphate and ammonium-nitrogen were highly correlated with the algal assemblage data.However,photosynthetic active radiation(PAR) showed poor correlation with the community data.These observations have cast some light on the autoecological characteristics,habitat preferences and environmental responses of tropical periphytic communities.
基金Supported by National Natural Science Foundation of China (No. 40821004)the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-213-3)National Basic Research Program of China (973 Program) (No. 2006CB400604)
文摘Flow cytometric determinations of the abundance distribution picophytoplankton (i.e. Prochlorococcus spp., orange fluorescence and community structure of Svnechococcus spp. and picoeukaryotes) were used for samples taken from the Philippine Sea in the western tropical Pacific Ocean from September to October of 2004. A fluorescence probe was employed to detect Chlorophyll a (Chl a). Abundances of Prochlorococcus spp., orange fluorescence Synechococcus spp. and picoeukaryotes ranged from 0.1 to 58×10^3 cells ml^-1, 0.38 to 17×10^2 cells ml^-1 and 0.42 to 26×10^2 cells ml^-1, respectively. Synechococcus spp. and picoeukaryotes co-occurred in relatively shallow water with the maximum abundance observed at 50 to 70 m depth, while Prochlorococcus spp. only occurred in the 70 to 200 m layer. Prochlorococcus spp. was the dominant picophytoplankton population in terms of abundance and biomass. The cell size and carbon biomass content were estimated for the three picophytoplankton groups. In addition, among the three groups of picophytoplankton, the relative contribution of red fluorescence to the total red fluorescence varied with depth. The fluorescence and light scatter properties of individual cells indicated that in the upper 100 m layer, picoeukaryotes were a major contributor to total red fluorescence, while at the depth below 100 m, Prochlorococcus spp. and Synechococcus spp. made an important contribution to the total red fluorescence.
基金Supported by the National Natural Science Foundation of China(No.30470344and30670088)Key Technology Project of Hubei Province(No.2006AA305A04)Commonweal Project of State Oceanic Administration of China(No.200705014)
文摘Virioplankton dynamics at seven sample sites(from two freshwater lakes) with different trophic states were monitored over the period of a year.Water was sampled monthly from August 2004 to July 2005,and the abundances of viruses and bacteria were measured using direct epifluorescence microscopy counting.Results indicated that both natural and anthropogenic factors could influence the distribution of virioplankton.Temporally,viral abundance was significantly correlated to bacterial density and water temperature.Spatially,viral abundance was significantly correlated to trophic state.This in turn indicated that viral abundance was directly dependant on host abundance in eutrophic lakes,while trophic state and temperature could drive the distribution of virioplankton.The virus-bacteria ratio was significantly lower in less productive water-bodies.The result implied that the control of virioplankton on their hosts may change according to the host density.