核医学科开展体外分析已近半个世纪，体外分析实验室已经成为临床医学实验室的重要组成部分。近年来，我国医学实验室认可工作进展迅速，自2005年以来全国已有230家实验室通过了认可，其中检验科175家，核医学科只有3家[数据来源于中国...核医学科开展体外分析已近半个世纪，体外分析实验室已经成为临床医学实验室的重要组成部分。近年来，我国医学实验室认可工作进展迅速，自2005年以来全国已有230家实验室通过了认可，其中检验科175家，核医学科只有3家[数据来源于中国合格评定国家认可委员会（China National Accreditation Service for Conformity Assessment, CNAS）官方网站：www.cnas.org.cn，截止到2016年10月12日]。多数核医学科存在重显像而忽视体外标记免疫分析的倾向，黄钢和盛世乐曾提出应重视和关注标记免疫分析在核医学发展中的地位。中华医学会核医学分会组织体外分析学组撰写了《核医学体外分析实验室管理规范》，旨在推动核医学实验室实现标准化、规范化管理，促进核医学体外分析技术的健康发展。实验室认可无疑是推动实验室标准化、规范化的一条捷径。现将本科实验室国际标准化组织（International Organization for Standardization, ISO）15189认可流程、认可意义以及笔者的体会等与业界同仁进行分享。展开更多
Objective:Weed pollens are common sources of allergens worldwide.The prevalence of weed pollen sensitization is not yet fully known in China.The purpose of this study was to investigate the prevalence of sensitization...Objective:Weed pollens are common sources of allergens worldwide.The prevalence of weed pollen sensitization is not yet fully known in China.The purpose of this study was to investigate the prevalence of sensitization to weed allergens from Artemisia,Ambrosia,and Humulus in northern China.Methods:A total of 1144 subjects(aged from 5 to 68 years) visiting our clinic from June to October 2011 underwent intradermal testing using a panel of 25 allergen sources.Subjects with positive skin responses to any pollen were further tested for their serum concentrations of IgE antibodies against Artemisia vulgaris,Ambrosia artemisiifolia,and Humulus scandens,and against the purified allergens,Art v 1 and Amb a 1.Results:Of 1144 subjects,170 had positive intradermal reactions to pollen and 144 donated serum for IgE testing.The prevalence of positive intradermal responses to pollens of Artemisia sieversiana,Artemisia annua,A.artemisiifolia,and H.scandens was 11.0%,10.2%,3.7%,and 6.6%,respectively.Among the intradermal positive subjects,the prevalence of specific IgE antigens to A.vulgaris was 58.3%,to A.artemisiifolia 14.7%,and to H.scandens 41.0%.The prevalence of specific IgE antigens to the allergen Art v 1 was 46.9%,and to Amb a 1 was 11.2%.The correlation between the presence of IgE antibodies specific to A.vulgaris and to the Art v 1 antigen was very high.Subjects with A.artemisiifolia specific IgE also had A.vulgaris specific IgE,but with relatively high levels of A.vulgaris IgE antibodies.There were no correlations between the presence of IgE antibodies to H.scandens and A.vulgaris or to H.scandens and A.artemisiifolia.Conclusions:The intradermal prevalence of weed pollen sensitization among allergic subjects in northern China is about 13.5%.Correlations of specific IgE antibodies suggest that pollen allergens from Artemisia and Humulus are independent sources for primary sensitization.展开更多
文摘核医学科开展体外分析已近半个世纪，体外分析实验室已经成为临床医学实验室的重要组成部分。近年来，我国医学实验室认可工作进展迅速，自2005年以来全国已有230家实验室通过了认可，其中检验科175家，核医学科只有3家[数据来源于中国合格评定国家认可委员会（China National Accreditation Service for Conformity Assessment, CNAS）官方网站：www.cnas.org.cn，截止到2016年10月12日]。多数核医学科存在重显像而忽视体外标记免疫分析的倾向，黄钢和盛世乐曾提出应重视和关注标记免疫分析在核医学发展中的地位。中华医学会核医学分会组织体外分析学组撰写了《核医学体外分析实验室管理规范》，旨在推动核医学实验室实现标准化、规范化管理，促进核医学体外分析技术的健康发展。实验室认可无疑是推动实验室标准化、规范化的一条捷径。现将本科实验室国际标准化组织（International Organization for Standardization, ISO）15189认可流程、认可意义以及笔者的体会等与业界同仁进行分享。
文摘Objective:Weed pollens are common sources of allergens worldwide.The prevalence of weed pollen sensitization is not yet fully known in China.The purpose of this study was to investigate the prevalence of sensitization to weed allergens from Artemisia,Ambrosia,and Humulus in northern China.Methods:A total of 1144 subjects(aged from 5 to 68 years) visiting our clinic from June to October 2011 underwent intradermal testing using a panel of 25 allergen sources.Subjects with positive skin responses to any pollen were further tested for their serum concentrations of IgE antibodies against Artemisia vulgaris,Ambrosia artemisiifolia,and Humulus scandens,and against the purified allergens,Art v 1 and Amb a 1.Results:Of 1144 subjects,170 had positive intradermal reactions to pollen and 144 donated serum for IgE testing.The prevalence of positive intradermal responses to pollens of Artemisia sieversiana,Artemisia annua,A.artemisiifolia,and H.scandens was 11.0%,10.2%,3.7%,and 6.6%,respectively.Among the intradermal positive subjects,the prevalence of specific IgE antigens to A.vulgaris was 58.3%,to A.artemisiifolia 14.7%,and to H.scandens 41.0%.The prevalence of specific IgE antigens to the allergen Art v 1 was 46.9%,and to Amb a 1 was 11.2%.The correlation between the presence of IgE antibodies specific to A.vulgaris and to the Art v 1 antigen was very high.Subjects with A.artemisiifolia specific IgE also had A.vulgaris specific IgE,but with relatively high levels of A.vulgaris IgE antibodies.There were no correlations between the presence of IgE antibodies to H.scandens and A.vulgaris or to H.scandens and A.artemisiifolia.Conclusions:The intradermal prevalence of weed pollen sensitization among allergic subjects in northern China is about 13.5%.Correlations of specific IgE antibodies suggest that pollen allergens from Artemisia and Humulus are independent sources for primary sensitization.
