Background: Because there are currently many effective therapies available for Sé zary syndrome, close monitoring of disease progression is required in order for a clinician to know when to institute or change an...Background: Because there are currently many effective therapies available for Sé zary syndrome, close monitoring of disease progression is required in order for a clinician to know when to institute or change an intervention. It has been our clinical experience that changes in patients’ CD4+ CD26- T- cell populations of peripheral blood lymphocytes herald changes in their clinical status. Objective: Our purpose was to evaluate whether a change in patients’ CD4+ CD26- population of T cells presages a change in their clinical status. We also sought to investigate the association between a change in Tcell populations that are CD4+ CD7- , CD8+ , CD56+ , and the CD4 + /CD8+ T- cell ratio and a change in the patient’ s clinical status. Methods: We conducted a retrospective chart review analysis of 21 patients with Sé zary syndrome who had flow cytometry, usually including levels of CD4+ CD26- , CD4+ CD7- , CD8+ , CD56+ , and CD4+ /CD8+ ratios measured at two time periods, 12 weeks apart. Results: We report two cases in which changes in patients’ clinical status were preceded by several weeks by a change in their CD4+ CD26- level. We report weak associations between a decreasing CD4+ CD26- T- cell population, a decreasing CD4+ CD7- population, an increasing CD56+ population, and an improving clinical status. We also report stronger associations between both a decreasing CD8+ population and an increasing CD4+ /CD8+ ratio and a worsening clinical status. Limitations: The study was limited by the number of patients and the time period over which the study was conducted. In addition, varying configurations of CD4+ CD26- T- cell populations were observed that may have limited the utility of this measurement. Conclusions: Flow cytometry assays of patients’ blood and, in particular, measurement of the CD4+ CD26- population of lymphocytes over time may be a valuable tool for monitoring patients with Sé zary syndrome. There exist varying configurations of CD26 T lymphocytes that may cause differences in standards for what is considered positive and negative between observers. Further prospective analysis involving larger groups of patients is recommended.展开更多
目的探讨手足口病（hand foot and mouth disease，HVMD）患儿血浆儿茶酚胺水平与临床病情及肠道病毒感染的关系。方法2014年4月至7月我院感染科确诊的129例HFMD患儿为研究对象。采集患儿静脉血2m1分离血浆，进行儿茶酚胺（包括去甲肾...目的探讨手足口病（hand foot and mouth disease，HVMD）患儿血浆儿茶酚胺水平与临床病情及肠道病毒感染的关系。方法2014年4月至7月我院感染科确诊的129例HFMD患儿为研究对象。采集患儿静脉血2m1分离血浆，进行儿茶酚胺（包括去甲肾上腺素、肾上腺素、多巴胺）含量测定，同时收集研究对象的粪便，采用荧光定量PCR方法进行大便病原检测。根据患儿的病情严重程度分为普通型组（n=20）、重型组（n=45）和危重型组（n=64），根据大便病原结果分为EV71阳性组（n=57），COA16阳性组（n=26），EV阳性组（n=10）、EV阴性组（n=16），有20例为柯萨奇病毒其他型，未进行分组。结果重型组[（175．07±42．26）pg／ml]和危重型组[（256．16±242．67）pg／ml]血浆去甲肾上腺素水平显著高于普通型组[（139．89±20．82）pg／ml]（P〈0.05），危重型组高于重型组（P〈0.05）。三组肾上腺素、多巴胺水平差异无统计学意义。在不同肠道病毒感染组中，儿茶酚胺水平的差异无统计学意义（P〉0.05）。结论儿茶酚胺参与了HFMD的发病机制，儿茶酚胺水平与病情严重程度有关，其中主要是去甲肾上腺素，可作为病情严重程度评估的指标，与病毒感染的类型无明显的相关性。展开更多
文摘Background: Because there are currently many effective therapies available for Sé zary syndrome, close monitoring of disease progression is required in order for a clinician to know when to institute or change an intervention. It has been our clinical experience that changes in patients’ CD4+ CD26- T- cell populations of peripheral blood lymphocytes herald changes in their clinical status. Objective: Our purpose was to evaluate whether a change in patients’ CD4+ CD26- population of T cells presages a change in their clinical status. We also sought to investigate the association between a change in Tcell populations that are CD4+ CD7- , CD8+ , CD56+ , and the CD4 + /CD8+ T- cell ratio and a change in the patient’ s clinical status. Methods: We conducted a retrospective chart review analysis of 21 patients with Sé zary syndrome who had flow cytometry, usually including levels of CD4+ CD26- , CD4+ CD7- , CD8+ , CD56+ , and CD4+ /CD8+ ratios measured at two time periods, 12 weeks apart. Results: We report two cases in which changes in patients’ clinical status were preceded by several weeks by a change in their CD4+ CD26- level. We report weak associations between a decreasing CD4+ CD26- T- cell population, a decreasing CD4+ CD7- population, an increasing CD56+ population, and an improving clinical status. We also report stronger associations between both a decreasing CD8+ population and an increasing CD4+ /CD8+ ratio and a worsening clinical status. Limitations: The study was limited by the number of patients and the time period over which the study was conducted. In addition, varying configurations of CD4+ CD26- T- cell populations were observed that may have limited the utility of this measurement. Conclusions: Flow cytometry assays of patients’ blood and, in particular, measurement of the CD4+ CD26- population of lymphocytes over time may be a valuable tool for monitoring patients with Sé zary syndrome. There exist varying configurations of CD26 T lymphocytes that may cause differences in standards for what is considered positive and negative between observers. Further prospective analysis involving larger groups of patients is recommended.
文摘目的探讨手足口病（hand foot and mouth disease，HVMD）患儿血浆儿茶酚胺水平与临床病情及肠道病毒感染的关系。方法2014年4月至7月我院感染科确诊的129例HFMD患儿为研究对象。采集患儿静脉血2m1分离血浆，进行儿茶酚胺（包括去甲肾上腺素、肾上腺素、多巴胺）含量测定，同时收集研究对象的粪便，采用荧光定量PCR方法进行大便病原检测。根据患儿的病情严重程度分为普通型组（n=20）、重型组（n=45）和危重型组（n=64），根据大便病原结果分为EV71阳性组（n=57），COA16阳性组（n=26），EV阳性组（n=10）、EV阴性组（n=16），有20例为柯萨奇病毒其他型，未进行分组。结果重型组[（175．07±42．26）pg／ml]和危重型组[（256．16±242．67）pg／ml]血浆去甲肾上腺素水平显著高于普通型组[（139．89±20．82）pg／ml]（P〈0.05），危重型组高于重型组（P〈0.05）。三组肾上腺素、多巴胺水平差异无统计学意义。在不同肠道病毒感染组中，儿茶酚胺水平的差异无统计学意义（P〉0.05）。结论儿茶酚胺参与了HFMD的发病机制，儿茶酚胺水平与病情严重程度有关，其中主要是去甲肾上腺素，可作为病情严重程度评估的指标，与病毒感染的类型无明显的相关性。
